Prevalence of copy-number neutral LOH in glioblastomas revealed by genomewide analysis of laser-microdissected tissues

Daisuke Kuga; Masahiro Mizoguchi; Yanlei Guan; Nobuhiro Hata; Koji Yoshimoto; Tadahisa Shono; Satoshi O. Suzuki; Yoji Kukita; Tomoko Tahira; Shinji Nagata; Tomio Sasaki; Kenshi Hayashi

doi:10.1215/15228517-2008-064

Prevalence of copy-number neutral LOH in glioblastomas revealed by genomewide analysis of laser-microdissected tissues

Daisuke Kuga, Masahiro Mizoguchi, Yanlei Guan, Nobuhiro Hata, Koji Yoshimoto, Tadahisa Shono, Satoshi O. Suzuki, Yoji Kukita, Tomoko Tahira, Shinji Nagata, Tomio Sasaki, Kenshi Hayashi

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

We have employed a laser-capture microdissection technique and single-nucleotide polymorphism arrays to characterize genomic alterations associated with the development of glioblastoma multiforme (GBM). Combined analysis of loss of heterozygosity (LOH) and copy number revealed that more than half (56.3%) of the 254 identified LOH loci showed no copy-number alteration, indicating the presence of copy-number neutral LOH (cnLOH). Furthermore, we found a GBM case that showed cnLOH in 18 of the 22 autosomes. These results were confirmed by quantitative real-time PCR, microsat-ellite analysis, and fluorescence in situ hybridization. The high rate of cnLOH suggests that epigenetic abnormalities of many genes are involved in the development and pro-gression of GBMs.

Original language	English
Pages (from-to)	995-1003
Number of pages	9
Journal	Neuro-oncology
Volume	10
Issue number	6
DOIs	https://doi.org/10.1215/15228517-2008-064
Publication status	Published - 2008 Dec
Externally published	Yes

Keywords

Copy-number alteration
Copy-number neutral loh
Glioblastoma
Loss of heterozygosity
Single-nucleotide polymorphism array

ASJC Scopus subject areas

Oncology
Clinical Neurology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1215/15228517-2008-064

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title = "Prevalence of copy-number neutral LOH in glioblastomas revealed by genomewide analysis of laser-microdissected tissues",

abstract = "We have employed a laser-capture microdissection technique and single-nucleotide polymorphism arrays to characterize genomic alterations associated with the development of glioblastoma multiforme (GBM). Combined analysis of loss of heterozygosity (LOH) and copy number revealed that more than half (56.3%) of the 254 identified LOH loci showed no copy-number alteration, indicating the presence of copy-number neutral LOH (cnLOH). Furthermore, we found a GBM case that showed cnLOH in 18 of the 22 autosomes. These results were confirmed by quantitative real-time PCR, microsat-ellite analysis, and fluorescence in situ hybridization. The high rate of cnLOH suggests that epigenetic abnormalities of many genes are involved in the development and pro-gression of GBMs.",

keywords = "Copy-number alteration, Copy-number neutral loh, Glioblastoma, Loss of heterozygosity, Single-nucleotide polymorphism array",

author = "Daisuke Kuga and Masahiro Mizoguchi and Yanlei Guan and Nobuhiro Hata and Koji Yoshimoto and Tadahisa Shono and Suzuki, {Satoshi O.} and Yoji Kukita and Tomoko Tahira and Shinji Nagata and Tomio Sasaki and Kenshi Hayashi",

year = "2008",

month = dec,

doi = "10.1215/15228517-2008-064",

language = "English",

volume = "10",

pages = "995--1003",

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TY - JOUR

T1 - Prevalence of copy-number neutral LOH in glioblastomas revealed by genomewide analysis of laser-microdissected tissues

AU - Kuga, Daisuke

AU - Mizoguchi, Masahiro

AU - Guan, Yanlei

AU - Hata, Nobuhiro

AU - Yoshimoto, Koji

AU - Shono, Tadahisa

AU - Suzuki, Satoshi O.

AU - Kukita, Yoji

AU - Tahira, Tomoko

AU - Nagata, Shinji

AU - Sasaki, Tomio

AU - Hayashi, Kenshi

PY - 2008/12

Y1 - 2008/12

N2 - We have employed a laser-capture microdissection technique and single-nucleotide polymorphism arrays to characterize genomic alterations associated with the development of glioblastoma multiforme (GBM). Combined analysis of loss of heterozygosity (LOH) and copy number revealed that more than half (56.3%) of the 254 identified LOH loci showed no copy-number alteration, indicating the presence of copy-number neutral LOH (cnLOH). Furthermore, we found a GBM case that showed cnLOH in 18 of the 22 autosomes. These results were confirmed by quantitative real-time PCR, microsat-ellite analysis, and fluorescence in situ hybridization. The high rate of cnLOH suggests that epigenetic abnormalities of many genes are involved in the development and pro-gression of GBMs.

AB - We have employed a laser-capture microdissection technique and single-nucleotide polymorphism arrays to characterize genomic alterations associated with the development of glioblastoma multiforme (GBM). Combined analysis of loss of heterozygosity (LOH) and copy number revealed that more than half (56.3%) of the 254 identified LOH loci showed no copy-number alteration, indicating the presence of copy-number neutral LOH (cnLOH). Furthermore, we found a GBM case that showed cnLOH in 18 of the 22 autosomes. These results were confirmed by quantitative real-time PCR, microsat-ellite analysis, and fluorescence in situ hybridization. The high rate of cnLOH suggests that epigenetic abnormalities of many genes are involved in the development and pro-gression of GBMs.

KW - Copy-number alteration

KW - Copy-number neutral loh

KW - Glioblastoma

KW - Loss of heterozygosity

KW - Single-nucleotide polymorphism array

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DO - 10.1215/15228517-2008-064

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SN - 1522-8517

VL - 10

SP - 995

EP - 1003

JO - Neuro-Oncology

JF - Neuro-Oncology

IS - 6

ER -

Prevalence of copy-number neutral LOH in glioblastomas revealed by genomewide analysis of laser-microdissected tissues

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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