Prevalence of copy-number neutral LOH in glioblastomas revealed by genomewide analysis of laser-microdissected tissues

Daisuke Kuga, Masahiro Mizoguchi, Yanlei Guan, Nobuhiro Hata, Koji Yoshimoto, Tadahisa Shono, Satoshi O. Suzuki, Yoji Kukita, Tomoko Tahira, Shinji Nagata, Tomio Sasaki, Kenshi Hayashi

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25 Citations (Scopus)

Abstract

We have employed a laser-capture microdissection technique and single-nucleotide polymorphism arrays to characterize genomic alterations associated with the development of glioblastoma multiforme (GBM). Combined analysis of loss of heterozygosity (LOH) and copy number revealed that more than half (56.3%) of the 254 identified LOH loci showed no copy-number alteration, indicating the presence of copy-number neutral LOH (cnLOH). Furthermore, we found a GBM case that showed cnLOH in 18 of the 22 autosomes. These results were confirmed by quantitative real-time PCR, microsat-ellite analysis, and fluorescence in situ hybridization. The high rate of cnLOH suggests that epigenetic abnormalities of many genes are involved in the development and pro-gression of GBMs.

Original languageEnglish
Pages (from-to)995-1003
Number of pages9
JournalNeuro-oncology
Volume10
Issue number6
DOIs
Publication statusPublished - 2008 Dec

Keywords

  • Copy-number alteration
  • Copy-number neutral loh
  • Glioblastoma
  • Loss of heterozygosity
  • Single-nucleotide polymorphism array

ASJC Scopus subject areas

  • Oncology
  • Clinical Neurology
  • Cancer Research

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    Kuga, D., Mizoguchi, M., Guan, Y., Hata, N., Yoshimoto, K., Shono, T., Suzuki, S. O., Kukita, Y., Tahira, T., Nagata, S., Sasaki, T., & Hayashi, K. (2008). Prevalence of copy-number neutral LOH in glioblastomas revealed by genomewide analysis of laser-microdissected tissues. Neuro-oncology, 10(6), 995-1003. https://doi.org/10.1215/15228517-2008-064