Prevalence of Entamoeba nuttalli infection in wild rhesus macaques in Nepal and characterization of the parasite isolates

Hiroshi Tachibana, Tetsuo Yanagi, Chamala Lama, Kishor Pandey, Meng Feng, Seiki Kobayashi, Jeevan B. Sherchand

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We have recently resurrected the name Entamoeba nuttalli Castellani, 1908 for a potentially virulent ameba isolate, P19-061405, obtained from a rhesus macaque in Kathmandu, Nepal. The ameba was morphologically indistinguishable from Entamoeba histolytica/. Entamoeba dispar/. Entamoeba moshkovskii, but located phylogenetically between E. histolytica and E. dispar. To evaluate the prevalence of E. nuttalli infection in wild rhesus macaques, 112 fecal samples were collected in four locations of the Kathmandu Valley. PCR analysis of DNA extracted from the feces showed positive rates of E. nuttalli, E. dispar, E. histolytica and E. moshkovskii of 51%, 12%, 0% and 0%, respectively. A total of 14 E. nuttalli isolates were obtained from four locations, of which 6 were established as axenic cultures. The sequences of the serine-rich protein gene of E. nuttalli isolates differed among four locations although no differences were found in the composition of sequence motifs. Isoenzyme pattern was analyzed in 8 isolates obtained from three locations. In hexokinase, the mobility of the slower migrating band was located between E. histolytica and E. dispar regardless of the culture conditions. These results demonstrate that E. nuttalli is highly prevalent in wild rhesus macaques in Nepal. Rhesus macaques appear to be one of the natural hosts and heterogeneity of the serine-rich protein gene might be useful for geographical typing of isolates.

Original languageEnglish
Pages (from-to)230-235
Number of pages6
JournalParasitology International
Volume62
Issue number2
DOIs
Publication statusPublished - 2013 Apr

Fingerprint

Entamoeba
Nepal
Entamoeba histolytica
Macaca mulatta
Parasites
Amoeba
Infection
Serine
Axenic Culture
Hexokinase
Feces
Isoenzymes
Names
Proteins
Polymerase Chain Reaction
DNA

Keywords

  • Entamoeba nuttalli
  • Nepal
  • Rhesus macaque
  • Serine-rich protein gene
  • Zymodeme

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Prevalence of Entamoeba nuttalli infection in wild rhesus macaques in Nepal and characterization of the parasite isolates. / Tachibana, Hiroshi; Yanagi, Tetsuo; Lama, Chamala; Pandey, Kishor; Feng, Meng; Kobayashi, Seiki; Sherchand, Jeevan B.

In: Parasitology International, Vol. 62, No. 2, 04.2013, p. 230-235.

Research output: Contribution to journalArticle

Tachibana, Hiroshi ; Yanagi, Tetsuo ; Lama, Chamala ; Pandey, Kishor ; Feng, Meng ; Kobayashi, Seiki ; Sherchand, Jeevan B. / Prevalence of Entamoeba nuttalli infection in wild rhesus macaques in Nepal and characterization of the parasite isolates. In: Parasitology International. 2013 ; Vol. 62, No. 2. pp. 230-235.
@article{88e520ca672a4d14b3e1c2b6d8923066,
title = "Prevalence of Entamoeba nuttalli infection in wild rhesus macaques in Nepal and characterization of the parasite isolates",
abstract = "We have recently resurrected the name Entamoeba nuttalli Castellani, 1908 for a potentially virulent ameba isolate, P19-061405, obtained from a rhesus macaque in Kathmandu, Nepal. The ameba was morphologically indistinguishable from Entamoeba histolytica/. Entamoeba dispar/. Entamoeba moshkovskii, but located phylogenetically between E. histolytica and E. dispar. To evaluate the prevalence of E. nuttalli infection in wild rhesus macaques, 112 fecal samples were collected in four locations of the Kathmandu Valley. PCR analysis of DNA extracted from the feces showed positive rates of E. nuttalli, E. dispar, E. histolytica and E. moshkovskii of 51{\%}, 12{\%}, 0{\%} and 0{\%}, respectively. A total of 14 E. nuttalli isolates were obtained from four locations, of which 6 were established as axenic cultures. The sequences of the serine-rich protein gene of E. nuttalli isolates differed among four locations although no differences were found in the composition of sequence motifs. Isoenzyme pattern was analyzed in 8 isolates obtained from three locations. In hexokinase, the mobility of the slower migrating band was located between E. histolytica and E. dispar regardless of the culture conditions. These results demonstrate that E. nuttalli is highly prevalent in wild rhesus macaques in Nepal. Rhesus macaques appear to be one of the natural hosts and heterogeneity of the serine-rich protein gene might be useful for geographical typing of isolates.",
keywords = "Entamoeba nuttalli, Nepal, Rhesus macaque, Serine-rich protein gene, Zymodeme",
author = "Hiroshi Tachibana and Tetsuo Yanagi and Chamala Lama and Kishor Pandey and Meng Feng and Seiki Kobayashi and Sherchand, {Jeevan B.}",
year = "2013",
month = "4",
doi = "10.1016/j.parint.2013.01.004",
language = "English",
volume = "62",
pages = "230--235",
journal = "Parasitology International",
issn = "1383-5769",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Prevalence of Entamoeba nuttalli infection in wild rhesus macaques in Nepal and characterization of the parasite isolates

AU - Tachibana, Hiroshi

AU - Yanagi, Tetsuo

AU - Lama, Chamala

AU - Pandey, Kishor

AU - Feng, Meng

AU - Kobayashi, Seiki

AU - Sherchand, Jeevan B.

PY - 2013/4

Y1 - 2013/4

N2 - We have recently resurrected the name Entamoeba nuttalli Castellani, 1908 for a potentially virulent ameba isolate, P19-061405, obtained from a rhesus macaque in Kathmandu, Nepal. The ameba was morphologically indistinguishable from Entamoeba histolytica/. Entamoeba dispar/. Entamoeba moshkovskii, but located phylogenetically between E. histolytica and E. dispar. To evaluate the prevalence of E. nuttalli infection in wild rhesus macaques, 112 fecal samples were collected in four locations of the Kathmandu Valley. PCR analysis of DNA extracted from the feces showed positive rates of E. nuttalli, E. dispar, E. histolytica and E. moshkovskii of 51%, 12%, 0% and 0%, respectively. A total of 14 E. nuttalli isolates were obtained from four locations, of which 6 were established as axenic cultures. The sequences of the serine-rich protein gene of E. nuttalli isolates differed among four locations although no differences were found in the composition of sequence motifs. Isoenzyme pattern was analyzed in 8 isolates obtained from three locations. In hexokinase, the mobility of the slower migrating band was located between E. histolytica and E. dispar regardless of the culture conditions. These results demonstrate that E. nuttalli is highly prevalent in wild rhesus macaques in Nepal. Rhesus macaques appear to be one of the natural hosts and heterogeneity of the serine-rich protein gene might be useful for geographical typing of isolates.

AB - We have recently resurrected the name Entamoeba nuttalli Castellani, 1908 for a potentially virulent ameba isolate, P19-061405, obtained from a rhesus macaque in Kathmandu, Nepal. The ameba was morphologically indistinguishable from Entamoeba histolytica/. Entamoeba dispar/. Entamoeba moshkovskii, but located phylogenetically between E. histolytica and E. dispar. To evaluate the prevalence of E. nuttalli infection in wild rhesus macaques, 112 fecal samples were collected in four locations of the Kathmandu Valley. PCR analysis of DNA extracted from the feces showed positive rates of E. nuttalli, E. dispar, E. histolytica and E. moshkovskii of 51%, 12%, 0% and 0%, respectively. A total of 14 E. nuttalli isolates were obtained from four locations, of which 6 were established as axenic cultures. The sequences of the serine-rich protein gene of E. nuttalli isolates differed among four locations although no differences were found in the composition of sequence motifs. Isoenzyme pattern was analyzed in 8 isolates obtained from three locations. In hexokinase, the mobility of the slower migrating band was located between E. histolytica and E. dispar regardless of the culture conditions. These results demonstrate that E. nuttalli is highly prevalent in wild rhesus macaques in Nepal. Rhesus macaques appear to be one of the natural hosts and heterogeneity of the serine-rich protein gene might be useful for geographical typing of isolates.

KW - Entamoeba nuttalli

KW - Nepal

KW - Rhesus macaque

KW - Serine-rich protein gene

KW - Zymodeme

UR - http://www.scopus.com/inward/record.url?scp=84873704302&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84873704302&partnerID=8YFLogxK

U2 - 10.1016/j.parint.2013.01.004

DO - 10.1016/j.parint.2013.01.004

M3 - Article

C2 - 23370534

AN - SCOPUS:84873704302

VL - 62

SP - 230

EP - 235

JO - Parasitology International

JF - Parasitology International

SN - 1383-5769

IS - 2

ER -