Prevention and reversal of delta-9-tetrahydrocannabinol induced depression of natural killer cell activity by interleukin-2

Steven Specter, Melodie Rivenbark, Catherine Newton, Yutaka Kawakami, Gerald Lancz

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The effects of delta-9-tetrahydrocannabinol (THC) on NK cell activity were studied. Previously, we reported that incubation of human peripheral blood mononuclear cells in THC resulted in an inhibition of natural killer (NK) cell activity. The present study examined the mechanism(s) of the decrease in NK cell activity. The inhibition of killing by NK cells was not due to a failure of NK cells to bind to K562 target cells. Furthermore, indomethacin did not abrogate the THC-mediated effect, suggesting that prostaglandins are not involved in the process leading to suppression of NK cell activity. However, NK activity was partially restored if cells, pretreated with THC, were washed to remove excess drug and then incubated overnight in fresh medium before assay. Addition of 1 - 100 U IL-2, either during pretreatment with THC or during overnight incubation, precluded or promoted the reversal of the inhibition of NK cell cytotoxicity. We conclude that the regulatory mechanism(s) involved in depression of NK cell cytotoxicity by THC is significantly influenced by IL-2.

Original languageEnglish
Pages (from-to)63-69
Number of pages7
JournalInternational Journal of Immunopharmacology
Volume11
Issue number1
DOIs
Publication statusPublished - 1989
Externally publishedYes

Fingerprint

Dronabinol
Natural Killer Cells
Interleukin-2
K562 Cells
Indomethacin
Prostaglandins
Blood Cells

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

Prevention and reversal of delta-9-tetrahydrocannabinol induced depression of natural killer cell activity by interleukin-2. / Specter, Steven; Rivenbark, Melodie; Newton, Catherine; Kawakami, Yutaka; Lancz, Gerald.

In: International Journal of Immunopharmacology, Vol. 11, No. 1, 1989, p. 63-69.

Research output: Contribution to journalArticle

Specter, Steven ; Rivenbark, Melodie ; Newton, Catherine ; Kawakami, Yutaka ; Lancz, Gerald. / Prevention and reversal of delta-9-tetrahydrocannabinol induced depression of natural killer cell activity by interleukin-2. In: International Journal of Immunopharmacology. 1989 ; Vol. 11, No. 1. pp. 63-69.
@article{4e5120c27f89457fbd6b75347754357d,
title = "Prevention and reversal of delta-9-tetrahydrocannabinol induced depression of natural killer cell activity by interleukin-2",
abstract = "The effects of delta-9-tetrahydrocannabinol (THC) on NK cell activity were studied. Previously, we reported that incubation of human peripheral blood mononuclear cells in THC resulted in an inhibition of natural killer (NK) cell activity. The present study examined the mechanism(s) of the decrease in NK cell activity. The inhibition of killing by NK cells was not due to a failure of NK cells to bind to K562 target cells. Furthermore, indomethacin did not abrogate the THC-mediated effect, suggesting that prostaglandins are not involved in the process leading to suppression of NK cell activity. However, NK activity was partially restored if cells, pretreated with THC, were washed to remove excess drug and then incubated overnight in fresh medium before assay. Addition of 1 - 100 U IL-2, either during pretreatment with THC or during overnight incubation, precluded or promoted the reversal of the inhibition of NK cell cytotoxicity. We conclude that the regulatory mechanism(s) involved in depression of NK cell cytotoxicity by THC is significantly influenced by IL-2.",
author = "Steven Specter and Melodie Rivenbark and Catherine Newton and Yutaka Kawakami and Gerald Lancz",
year = "1989",
doi = "10.1016/0192-0561(89)90100-8",
language = "English",
volume = "11",
pages = "63--69",
journal = "International Immunopharmacology",
issn = "1567-5769",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Prevention and reversal of delta-9-tetrahydrocannabinol induced depression of natural killer cell activity by interleukin-2

AU - Specter, Steven

AU - Rivenbark, Melodie

AU - Newton, Catherine

AU - Kawakami, Yutaka

AU - Lancz, Gerald

PY - 1989

Y1 - 1989

N2 - The effects of delta-9-tetrahydrocannabinol (THC) on NK cell activity were studied. Previously, we reported that incubation of human peripheral blood mononuclear cells in THC resulted in an inhibition of natural killer (NK) cell activity. The present study examined the mechanism(s) of the decrease in NK cell activity. The inhibition of killing by NK cells was not due to a failure of NK cells to bind to K562 target cells. Furthermore, indomethacin did not abrogate the THC-mediated effect, suggesting that prostaglandins are not involved in the process leading to suppression of NK cell activity. However, NK activity was partially restored if cells, pretreated with THC, were washed to remove excess drug and then incubated overnight in fresh medium before assay. Addition of 1 - 100 U IL-2, either during pretreatment with THC or during overnight incubation, precluded or promoted the reversal of the inhibition of NK cell cytotoxicity. We conclude that the regulatory mechanism(s) involved in depression of NK cell cytotoxicity by THC is significantly influenced by IL-2.

AB - The effects of delta-9-tetrahydrocannabinol (THC) on NK cell activity were studied. Previously, we reported that incubation of human peripheral blood mononuclear cells in THC resulted in an inhibition of natural killer (NK) cell activity. The present study examined the mechanism(s) of the decrease in NK cell activity. The inhibition of killing by NK cells was not due to a failure of NK cells to bind to K562 target cells. Furthermore, indomethacin did not abrogate the THC-mediated effect, suggesting that prostaglandins are not involved in the process leading to suppression of NK cell activity. However, NK activity was partially restored if cells, pretreated with THC, were washed to remove excess drug and then incubated overnight in fresh medium before assay. Addition of 1 - 100 U IL-2, either during pretreatment with THC or during overnight incubation, precluded or promoted the reversal of the inhibition of NK cell cytotoxicity. We conclude that the regulatory mechanism(s) involved in depression of NK cell cytotoxicity by THC is significantly influenced by IL-2.

UR - http://www.scopus.com/inward/record.url?scp=0024587260&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024587260&partnerID=8YFLogxK

U2 - 10.1016/0192-0561(89)90100-8

DO - 10.1016/0192-0561(89)90100-8

M3 - Article

C2 - 2540100

AN - SCOPUS:0024587260

VL - 11

SP - 63

EP - 69

JO - International Immunopharmacology

JF - International Immunopharmacology

SN - 1567-5769

IS - 1

ER -