Primed immune responses to gram-negative peptidoglycans confer infection resistance in silkworms

Atsushi Miyashita, Hayato Kizaki, Kiyoshi Kawasaki, Kazuhisa Sekimizu, Chikara Kaito

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A heightened immune response, in which immune responses are primed by repeated exposure to a pathogen, is an important characteristic of vertebrate adaptive immunity. In the present study, we examined whether invertebrate animals also exhibit a primed immune response. The LD50 of Gram-negative enterohemorrhagic Escherichia coli O157:H7 Sakai in silkworms was increased 100-fold by pre-injection of heat-killed Sakai cells. Silkworms pre-injected with heat-killed cells of a Gram-positive bacterium, Staphylococcus aureus, did not have resistance to Sakai. Silkworms preinjected with enterohemorrhagic E. coli peptidoglycans, cell surface components of bacteria, were resistant to Sakai infection. Silkworms preinjected with S. aureus peptidoglycans, however, were not resistant to Sakai. Silkworms preinjected with heat-killed Sakai cells showed persistent resistance to Sakai infection even after pupation. Repeated injection of heat-killed Sakai cells into the silkworms induced earlier and greater production of antimicrobial peptides than a single injection of heat-killed Sakai cells. These findings suggest that silkworm recognition of Gram-negative peptidoglycans leads to a primed immune reaction and increased resistance to a second round of bacterial infection.

Original languageEnglish
Pages (from-to)14412-14421
Number of pages10
JournalJournal of Biological Chemistry
Volume289
Issue number20
DOIs
Publication statusPublished - 2014 May 16
Externally publishedYes

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Bombyx
Peptidoglycan
Hot Temperature
Infection
Enterohemorrhagic Escherichia coli
Escherichia coli
Bacteria
Antigen-antibody reactions
Injections
Staphylococcus aureus
Pathogens
Escherichia coli O157
Animals
Lethal Dose 50
Gram-Positive Bacteria
Adaptive Immunity
Cellular Structures
Invertebrates
Bacterial Infections
Vertebrates

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Primed immune responses to gram-negative peptidoglycans confer infection resistance in silkworms. / Miyashita, Atsushi; Kizaki, Hayato; Kawasaki, Kiyoshi; Sekimizu, Kazuhisa; Kaito, Chikara.

In: Journal of Biological Chemistry, Vol. 289, No. 20, 16.05.2014, p. 14412-14421.

Research output: Contribution to journalArticle

Miyashita, Atsushi ; Kizaki, Hayato ; Kawasaki, Kiyoshi ; Sekimizu, Kazuhisa ; Kaito, Chikara. / Primed immune responses to gram-negative peptidoglycans confer infection resistance in silkworms. In: Journal of Biological Chemistry. 2014 ; Vol. 289, No. 20. pp. 14412-14421.
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