TY - JOUR
T1 - Priming of alveolar macrophages for interleukin-8 production in patients with idiopathic pulmonary fibrosis
AU - Nakamura, Hidetoshi
AU - Fujishima, Seitaro
AU - Waki, Yasuhiro
AU - Urano, Tetsuya
AU - Sayama, Koichi
AU - Sakamaki, Fumio
AU - Terashima, Takeshi
AU - Soejima, Kenzo
AU - Tasaka, Sadatomo
AU - Ishizaka, Akitoshi
AU - Kawashiro, Takeo
AU - Kanazawa, Minoru
PY - 1995/11
Y1 - 1995/11
N2 - We evaluated the contribution of interleukin-8 (IL-8) to the pathogenesis of idiopathic pulmonary fibrosis (IPF) by studying bronchoalveolar lavage fluid (BALF) in eight patients with IPF in the chronically progressive phase, five patients with IPF in the subacutely progressive phase, eight patients with sarcoidosis (SAR), and eight control (CTL) subjects. IL-8 levels were not increased in the BALF of the patients with IPF in the chronic phase (11.3 ± 8.8 pg/ml), nor in that of the SAR patients (13.8 ± 7.8 pg/ml), whereas they were increased in the BALF of patients with IPF in the subacutely progressive phase (1.93 ± 1.10 ng/ml). We then investigated extracellular and cell-associated IL-8 in lipopolysaccharide (LPS)-stimulated BALF cells to determine the IL-8-producing potential of alveolar macrophages (AM). Following LPS stimulation of BALF cells from patients with IPF in the chronic phase, both the extracellular IL-8 in culture fluid and the cell-associated IL-8 in AM were increased as compared with those for the CTL subjects (p< 0.05 and p< 0.05, respectively). These results suggest that AM of patients with IPF are primed for IL-8 production. We conclude that IL-8 may play a role in neutrophilic alveolitis, especially during the subacute phase of IPF.
AB - We evaluated the contribution of interleukin-8 (IL-8) to the pathogenesis of idiopathic pulmonary fibrosis (IPF) by studying bronchoalveolar lavage fluid (BALF) in eight patients with IPF in the chronically progressive phase, five patients with IPF in the subacutely progressive phase, eight patients with sarcoidosis (SAR), and eight control (CTL) subjects. IL-8 levels were not increased in the BALF of the patients with IPF in the chronic phase (11.3 ± 8.8 pg/ml), nor in that of the SAR patients (13.8 ± 7.8 pg/ml), whereas they were increased in the BALF of patients with IPF in the subacutely progressive phase (1.93 ± 1.10 ng/ml). We then investigated extracellular and cell-associated IL-8 in lipopolysaccharide (LPS)-stimulated BALF cells to determine the IL-8-producing potential of alveolar macrophages (AM). Following LPS stimulation of BALF cells from patients with IPF in the chronic phase, both the extracellular IL-8 in culture fluid and the cell-associated IL-8 in AM were increased as compared with those for the CTL subjects (p< 0.05 and p< 0.05, respectively). These results suggest that AM of patients with IPF are primed for IL-8 production. We conclude that IL-8 may play a role in neutrophilic alveolitis, especially during the subacute phase of IPF.
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U2 - 10.1164/ajrccm.152.5.7582298
DO - 10.1164/ajrccm.152.5.7582298
M3 - Article
C2 - 7582298
AN - SCOPUS:0029200732
SN - 1073-449X
VL - 152
SP - 1579
EP - 1586
JO - American Review of Respiratory Disease
JF - American Review of Respiratory Disease
IS - 5 I
ER -