Priming of alveolar macrophages for interleukin-8 production in patients with idiopathic pulmonary fibrosis

Hidetoshi Nakamura, Seitaro Fujishima, Yasuhiro Waki, Tetsuya Urano, Koichi Sayama, Fumio Sakamaki, Takeshi Terashima, Kenzo Soejima, Sadatomo Tasaka, Akitoshi Ishizaka, Takeo Kawashiro, Minoru Kanazawa

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42 Citations (Scopus)

Abstract

We evaluated the contribution of interleukin-8 (IL-8) to the pathogenesis of idiopathic pulmonary fibrosis (IPF) by studying bronchoalveolar lavage fluid (BALF) in eight patients with IPF in the chronically progressive phase, five patients with IPF in the subacutely progressive phase, eight patients with sarcoidosis (SAR), and eight control (CTL) subjects. IL-8 levels were not increased in the BALF of the patients with IPF in the chronic phase (11.3 ± 8.8 pg/ml), nor in that of the SAR patients (13.8 ± 7.8 pg/ml), whereas they were increased in the BALF of patients with IPF in the subacutely progressive phase (1.93 ± 1.10 ng/ml). We then investigated extracellular and cell-associated IL-8 in lipopolysaccharide (LPS)-stimulated BALF cells to determine the IL-8-producing potential of alveolar macrophages (AM). Following LPS stimulation of BALF cells from patients with IPF in the chronic phase, both the extracellular IL-8 in culture fluid and the cell-associated IL-8 in AM were increased as compared with those for the CTL subjects (p< 0.05 and p< 0.05, respectively). These results suggest that AM of patients with IPF are primed for IL-8 production. We conclude that IL-8 may play a role in neutrophilic alveolitis, especially during the subacute phase of IPF.

Original languageEnglish
Pages (from-to)1579-1586
Number of pages8
JournalAmerican journal of respiratory and critical care medicine
Volume152
Issue number5 I
DOIs
Publication statusPublished - 1995 Nov

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

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