Printed low-cost microfluidic analytical devices based on a transparent substrate

Shogo Fujisaki, Hiroyuki Shibata, Kentaro Yamada, Koji Suzuki, Daniel Citterio

Research output: Contribution to journalArticle

Abstract

This work describes the development of a microfluidic analytical device prepared on a transparent OHP film substrate, named the microfluidic transparent film-based analytical device (μTFAD). Printing technologies including wax printing for microchannel patterning and inkjet printing for chemical assay component deposition have been employed for the μTFAD fabrication. The fully printed μTFAD allowed gravity-assisted pump-free transportation of the sample liquid (50 μL) and an absorbance measurement-based iron ion (Fe 2+ ) assay using nitroso-PSAP as the colorimetric reagent within a wax-patterned microfluidic structure. By measuring absorbance values at the Fe 2+ -nitroso-PSAP complex-specific wavelength (756 nm), a response curve with a linear range of 0-200 μM was obtained. The limit of detection (1.18 μM) obtained with the proposed μTFADs was comparable to the results achieved with a conventional 96-well microplate assay (0.92 μM) and lower than that in the case of digital colour analysis-assisted filter paper spot tests (7.71 μM) or the absorbance analysis of refractive index-matched translucent filter paper spots (37.2 μM). In addition, highly selective Fe 2+ detection has been achieved in the presence of potentially interfering metal ions (Cu 2+ , Co 2+ , Ni 2+ ) without the use of any masking reagents, owing to the selection of the target complex-specific wavelength in the absorbance measurement on μTFADs.

Original languageEnglish
Pages (from-to)2746-2754
Number of pages9
JournalAnalyst
Volume144
Issue number8
DOIs
Publication statusPublished - 2019 Apr 21

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Lab-On-A-Chip Devices
Printing
absorbance
Microfluidics
Waxes
Costs and Cost Analysis
substrate
Assays
Equipment and Supplies
assay
Substrates
wax
cost
Ions
Costs
Refractometry
Gravitation
filter
wavelength
Wavelength

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Printed low-cost microfluidic analytical devices based on a transparent substrate. / Fujisaki, Shogo; Shibata, Hiroyuki; Yamada, Kentaro; Suzuki, Koji; Citterio, Daniel.

In: Analyst, Vol. 144, No. 8, 21.04.2019, p. 2746-2754.

Research output: Contribution to journalArticle

Fujisaki, Shogo ; Shibata, Hiroyuki ; Yamada, Kentaro ; Suzuki, Koji ; Citterio, Daniel. / Printed low-cost microfluidic analytical devices based on a transparent substrate. In: Analyst. 2019 ; Vol. 144, No. 8. pp. 2746-2754.
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