Abstract
Hypergammaglobulinemia and autoantibodies are reduced in pristane-treated specific pathogen-free mice vs. conventionally housed controls, consistent with the role of microbial stimulation in this model. To determine whether microbial stimulation is required, BALB/c mice housed under germ-free conditions were treated i.p. with sterile PBS or pristane and examined 6 months later. As in conventional mice, pristane-treated germ-free mice developed peritoneal granulomas and hypergammaglobulinemia with increased IgG2a/IgG1 ratios. LPS stimulation induced more IL-6, IL-12, and TNF-α, and anti-CD3 induced more IFN-γ and IL-4 by peritoneal cells from pristane-treated mice vs. control. Anti-nRNP/Sm and -Su autoantibodies were found in 40% and 43%, respectively, of pristane-treated germ-free mice by immunoprecipitation. Thus, bacterial stimulation was not required for lupus autoantibodies, peritoneal granuloma formation, hypergammaglobulinemia, or cytokine overproduction. Although microbial stimulation acts synergistically with pristane, these results clearly indicate that pristane does not act merely by increasing exposure to microbial products such as LPS.
| Original language | English |
|---|---|
| Pages (from-to) | 110-118 |
| Number of pages | 9 |
| Journal | Clinical Immunology |
| Volume | 114 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2005 Feb |
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Keywords
- Autoantibodies
- Cytokines
- Inflammation
- Lupus
- Microbial environment
- Pristane
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
Cite this
Pristane-induced autoimmunity in germ-free mice. / Mizutani, Akiei; Shaheen, Victoria M.; Yoshida, Hideo; Akaogi, Jun; Kuroda, Yoshiki; Nacionales, Dina C.; Yamasaki, Yoshioki; Hirakata, Michito; Ono, Nobutaka; Reeves, Westley H.; Satoh, Minoru.
In: Clinical Immunology, Vol. 114, No. 2, 02.2005, p. 110-118.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pristane-induced autoimmunity in germ-free mice
AU - Mizutani, Akiei
AU - Shaheen, Victoria M.
AU - Yoshida, Hideo
AU - Akaogi, Jun
AU - Kuroda, Yoshiki
AU - Nacionales, Dina C.
AU - Yamasaki, Yoshioki
AU - Hirakata, Michito
AU - Ono, Nobutaka
AU - Reeves, Westley H.
AU - Satoh, Minoru
PY - 2005/2
Y1 - 2005/2
N2 - Hypergammaglobulinemia and autoantibodies are reduced in pristane-treated specific pathogen-free mice vs. conventionally housed controls, consistent with the role of microbial stimulation in this model. To determine whether microbial stimulation is required, BALB/c mice housed under germ-free conditions were treated i.p. with sterile PBS or pristane and examined 6 months later. As in conventional mice, pristane-treated germ-free mice developed peritoneal granulomas and hypergammaglobulinemia with increased IgG2a/IgG1 ratios. LPS stimulation induced more IL-6, IL-12, and TNF-α, and anti-CD3 induced more IFN-γ and IL-4 by peritoneal cells from pristane-treated mice vs. control. Anti-nRNP/Sm and -Su autoantibodies were found in 40% and 43%, respectively, of pristane-treated germ-free mice by immunoprecipitation. Thus, bacterial stimulation was not required for lupus autoantibodies, peritoneal granuloma formation, hypergammaglobulinemia, or cytokine overproduction. Although microbial stimulation acts synergistically with pristane, these results clearly indicate that pristane does not act merely by increasing exposure to microbial products such as LPS.
AB - Hypergammaglobulinemia and autoantibodies are reduced in pristane-treated specific pathogen-free mice vs. conventionally housed controls, consistent with the role of microbial stimulation in this model. To determine whether microbial stimulation is required, BALB/c mice housed under germ-free conditions were treated i.p. with sterile PBS or pristane and examined 6 months later. As in conventional mice, pristane-treated germ-free mice developed peritoneal granulomas and hypergammaglobulinemia with increased IgG2a/IgG1 ratios. LPS stimulation induced more IL-6, IL-12, and TNF-α, and anti-CD3 induced more IFN-γ and IL-4 by peritoneal cells from pristane-treated mice vs. control. Anti-nRNP/Sm and -Su autoantibodies were found in 40% and 43%, respectively, of pristane-treated germ-free mice by immunoprecipitation. Thus, bacterial stimulation was not required for lupus autoantibodies, peritoneal granuloma formation, hypergammaglobulinemia, or cytokine overproduction. Although microbial stimulation acts synergistically with pristane, these results clearly indicate that pristane does not act merely by increasing exposure to microbial products such as LPS.
KW - Autoantibodies
KW - Cytokines
KW - Inflammation
KW - Lupus
KW - Microbial environment
KW - Pristane
UR - http://www.scopus.com/inward/record.url?scp=19944429660&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=19944429660&partnerID=8YFLogxK
U2 - 10.1016/j.clim.2004.09.010
DO - 10.1016/j.clim.2004.09.010
M3 - Article
C2 - 15639644
AN - SCOPUS:19944429660
VL - 114
SP - 110
EP - 118
JO - Clinical Immunology
JF - Clinical Immunology
SN - 1521-6616
IS - 2
ER -