Processing of pre-microRNAs by the Dicer-1-Loquacious complex in drosophila cells

Kuniaki Saito, Akira Ishizuka, Haruhiko Siomi, Mikiko C. Siomi

Research output: Contribution to journalArticle

294 Citations (Scopus)

Abstract

microRNAs (miRNAs) are a large family of 21- to 22-nucleotide non-coding RNAs that interact with target mRNAs at specific sites to induce cleavage of the message or inhibit translation. miRNAs are excised in a stepwise process from primary miRNA (pri-miRNA) transcripts. The Drosha-Pasha/DGCR8 complex in the nucleus cleaves pri-miRNAs to release hairpin-shaped precursor miRNAs (pre-miRNAs). These pre-miRNAs are then exported to the cytoplasm and further processed by Dicer to mature miRNAs. Here we show that Drosophila Dicer-1 interacts with Loquacious, a double-stranded RNA-binding domain protein. Depletion of Loquacious results in pre-miRNA accumulation in Drosophila S2 cells, as is the case for depletion of Dicer-1. Immuno-affinity purification experiments revealed that along with Dicer-1, Loquacious resides in a functional pre-miRNA processing complex, and stimulates and directs the specific pre-miRNA processing activity. These results support a model in which Loquacious mediates miRNA biogenesis and, thereby, the expression of genes regulated by miRNAs.

Original languageEnglish
Pages (from-to)1202-1212
Number of pages11
JournalPLoS Biology
Volume3
Issue number7
DOIs
Publication statusPublished - 2005 Jul
Externally publishedYes

Fingerprint

MicroRNAs
microRNA
Drosophila
Processing
cells
Untranslated RNA
double-stranded RNA
RNA-Binding Proteins
Double-Stranded RNA
translation (genetics)
Purification
cytoplasm
nucleotides
Cytoplasm
Nucleotides
Genes
gene expression
Gene Expression
Messenger RNA

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Processing of pre-microRNAs by the Dicer-1-Loquacious complex in drosophila cells. / Saito, Kuniaki; Ishizuka, Akira; Siomi, Haruhiko; Siomi, Mikiko C.

In: PLoS Biology, Vol. 3, No. 7, 07.2005, p. 1202-1212.

Research output: Contribution to journalArticle

Saito, Kuniaki ; Ishizuka, Akira ; Siomi, Haruhiko ; Siomi, Mikiko C. / Processing of pre-microRNAs by the Dicer-1-Loquacious complex in drosophila cells. In: PLoS Biology. 2005 ; Vol. 3, No. 7. pp. 1202-1212.
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