Production and characterization of transgenic mice harboring mutant human UMOD gene

Yuichi Takiue, Makoto Hosoyamada, Takuya Yokoo, Masaki Kimura, Manami Ochiai, Kiyoko Kaneko, Kimiyoshi Ichida, Tatsuo Hosoya, Toshiaki Shibasaki

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Familial juvenile hyperuricemic nephropathy is caused by mutations in the UMOD gene encoding uromodulin. A transgenic mouse model was developed by introducing a human mutant UMOD (C148W) cDNA under control of the mouse umod promoter. Uromodulin accumulation was observed in the thick ascending limb cells in the kidney of transgenic mice. However, the urinary excretion of uromodulin in transgenic mice did not decrease and LC-MS/MS analysis indicated it was of mouse origin. Moreover, the creatinine clearance was not different between wildtype and transgenic animals. Consequently, the onset of the disease was not observed in transgenic mice until 24 weeks of age.

Original languageEnglish
Pages (from-to)596-600
Number of pages5
JournalNucleosides, Nucleotides and Nucleic Acids
Volume27
Issue number6-7
DOIs
Publication statusPublished - 2008 Jun

Fingerprint

Uromodulin
Transgenic Mice
Genes
Gene encoding
Genetically Modified Animals
Creatinine
Animals
Complementary DNA
Extremities
Kidney
Mutation

Keywords

  • Creatinine clearance
  • Familial juvenile hyperuricemic nephropathy
  • LC-MS/MS
  • Transgenic mice
  • Uromodulin

ASJC Scopus subject areas

  • Genetics
  • Biochemistry

Cite this

Production and characterization of transgenic mice harboring mutant human UMOD gene. / Takiue, Yuichi; Hosoyamada, Makoto; Yokoo, Takuya; Kimura, Masaki; Ochiai, Manami; Kaneko, Kiyoko; Ichida, Kimiyoshi; Hosoya, Tatsuo; Shibasaki, Toshiaki.

In: Nucleosides, Nucleotides and Nucleic Acids, Vol. 27, No. 6-7, 06.2008, p. 596-600.

Research output: Contribution to journalArticle

Takiue, Y, Hosoyamada, M, Yokoo, T, Kimura, M, Ochiai, M, Kaneko, K, Ichida, K, Hosoya, T & Shibasaki, T 2008, 'Production and characterization of transgenic mice harboring mutant human UMOD gene', Nucleosides, Nucleotides and Nucleic Acids, vol. 27, no. 6-7, pp. 596-600. https://doi.org/10.1080/15257770802136065
Takiue, Yuichi ; Hosoyamada, Makoto ; Yokoo, Takuya ; Kimura, Masaki ; Ochiai, Manami ; Kaneko, Kiyoko ; Ichida, Kimiyoshi ; Hosoya, Tatsuo ; Shibasaki, Toshiaki. / Production and characterization of transgenic mice harboring mutant human UMOD gene. In: Nucleosides, Nucleotides and Nucleic Acids. 2008 ; Vol. 27, No. 6-7. pp. 596-600.
@article{db33e3c9234c46e9aae1d108bfbfe494,
title = "Production and characterization of transgenic mice harboring mutant human UMOD gene",
abstract = "Familial juvenile hyperuricemic nephropathy is caused by mutations in the UMOD gene encoding uromodulin. A transgenic mouse model was developed by introducing a human mutant UMOD (C148W) cDNA under control of the mouse umod promoter. Uromodulin accumulation was observed in the thick ascending limb cells in the kidney of transgenic mice. However, the urinary excretion of uromodulin in transgenic mice did not decrease and LC-MS/MS analysis indicated it was of mouse origin. Moreover, the creatinine clearance was not different between wildtype and transgenic animals. Consequently, the onset of the disease was not observed in transgenic mice until 24 weeks of age.",
keywords = "Creatinine clearance, Familial juvenile hyperuricemic nephropathy, LC-MS/MS, Transgenic mice, Uromodulin",
author = "Yuichi Takiue and Makoto Hosoyamada and Takuya Yokoo and Masaki Kimura and Manami Ochiai and Kiyoko Kaneko and Kimiyoshi Ichida and Tatsuo Hosoya and Toshiaki Shibasaki",
year = "2008",
month = "6",
doi = "10.1080/15257770802136065",
language = "English",
volume = "27",
pages = "596--600",
journal = "Nucleosides and Nucleotides",
issn = "0732-8311",
publisher = "Marcel Dekker Inc.",
number = "6-7",

}

TY - JOUR

T1 - Production and characterization of transgenic mice harboring mutant human UMOD gene

AU - Takiue, Yuichi

AU - Hosoyamada, Makoto

AU - Yokoo, Takuya

AU - Kimura, Masaki

AU - Ochiai, Manami

AU - Kaneko, Kiyoko

AU - Ichida, Kimiyoshi

AU - Hosoya, Tatsuo

AU - Shibasaki, Toshiaki

PY - 2008/6

Y1 - 2008/6

N2 - Familial juvenile hyperuricemic nephropathy is caused by mutations in the UMOD gene encoding uromodulin. A transgenic mouse model was developed by introducing a human mutant UMOD (C148W) cDNA under control of the mouse umod promoter. Uromodulin accumulation was observed in the thick ascending limb cells in the kidney of transgenic mice. However, the urinary excretion of uromodulin in transgenic mice did not decrease and LC-MS/MS analysis indicated it was of mouse origin. Moreover, the creatinine clearance was not different between wildtype and transgenic animals. Consequently, the onset of the disease was not observed in transgenic mice until 24 weeks of age.

AB - Familial juvenile hyperuricemic nephropathy is caused by mutations in the UMOD gene encoding uromodulin. A transgenic mouse model was developed by introducing a human mutant UMOD (C148W) cDNA under control of the mouse umod promoter. Uromodulin accumulation was observed in the thick ascending limb cells in the kidney of transgenic mice. However, the urinary excretion of uromodulin in transgenic mice did not decrease and LC-MS/MS analysis indicated it was of mouse origin. Moreover, the creatinine clearance was not different between wildtype and transgenic animals. Consequently, the onset of the disease was not observed in transgenic mice until 24 weeks of age.

KW - Creatinine clearance

KW - Familial juvenile hyperuricemic nephropathy

KW - LC-MS/MS

KW - Transgenic mice

KW - Uromodulin

UR - http://www.scopus.com/inward/record.url?scp=46749135161&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=46749135161&partnerID=8YFLogxK

U2 - 10.1080/15257770802136065

DO - 10.1080/15257770802136065

M3 - Article

C2 - 18600511

AN - SCOPUS:46749135161

VL - 27

SP - 596

EP - 600

JO - Nucleosides and Nucleotides

JF - Nucleosides and Nucleotides

SN - 0732-8311

IS - 6-7

ER -