Production of cell sheets and controlling of cellular structures by using particle monolayers

Manabu Miura, Keiji Fujimoto

Research output: Contribution to conferencePaper

Abstract

Using the particle monolayers, we intended to control, cellular adhesion, shape and structure. To make a cell sheet, cells were seeded onto a particle monolayer at the high density. Cells were spontaneously detached from the culture dish just by shaking. Then, BSA molecules were immobilized onto the particle monolayor by the photolithography. Endothdial cells were seeded on the patterned particle monolayer. It could be observed that cells preferentially adhered on the region where BSA. molecules were not immobilized. Individual cells were extended to a certain, direction and it seemed that their assembly was oriented in the same direction.

Original languageEnglish
Number of pages1
Publication statusPublished - 2006 Oct 19
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: 2006 May 242006 May 26

Other

Other55th SPSJ Annual Meeting
CountryJapan
CityNagoya
Period06/5/2406/5/26

Keywords

  • Cell sheet
  • Cellular structure
  • Particle monolayer
  • Patterning
  • Tissue regeneration

ASJC Scopus subject areas

  • Engineering(all)

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    Miura, M., & Fujimoto, K. (2006). Production of cell sheets and controlling of cellular structures by using particle monolayers. Paper presented at 55th SPSJ Annual Meeting, Nagoya, Japan.