Production of endothelin in human cancer cell lines

Masatoshi Kusuhara, Ken Yamaguchi, Koichi Nagasaki, Chiaki Hayashi, Ai Suzaki, Shingo Hori, Shunnosuke Handa, Yoshiro Nakamura, Kaoru Abe

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

Endothelin (ET)-1 is a vasoconstrictor peptide derived from endothelial cells and now known to be a local regulator of vascular tonus. Recent studies, however, have revealed that ET-1 functions also as growth factor in various cells. By using a specific ET-1 radioimmunoassay, immunoreactive (IR) ET-1, ranging from 4.2 to 150 pM (minimum detectable amount, 4.0 pM), was detected in 13 of 42 human cancer cell lines. The frequencies of IR-ET-1 production and its concentrations were high in mammary, pancreatic, and colon carcinoma cell lines. IR-ET-1 produced by cancer cells possessed the same molecular size as synthetic ET-1 and also had ET-1-like biological activity. Moreover, Northern blot analysis revealed bands corresponding to ET-1 mRNA in cancer cell lines, indicating that IR-ET-1 produced by cancer cells is a product of the ET-1 gene. Since ET-1 in the spent media is present in a sufficient amount to stimulate cellular growth, we sought ET-1 receptors in four pancreatic carcinoma cell lines and human skin fibroblasts. No ET-1 receptors were detected in the pancreatic carcinoma cell lines. However, human skin fibroblasts possessed a large number of ET-1 receptors. This finding raises the possibility that ET-1 produced by cancer cells plays a modulatory role in the growth of stromal cells surrounding cancer cells.

Original languageEnglish
Pages (from-to)3257-3261
Number of pages5
JournalCancer Research
Volume50
Issue number11
Publication statusPublished - 1990 Jun 1

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Endothelins
Endothelin-1
Cell Line
Neoplasms
Endothelin A Receptors
Fibroblasts
Skin
Vasoconstrictor Agents
Stromal Cells
Growth
Northern Blotting
Radioimmunoassay
Blood Vessels
Intercellular Signaling Peptides and Proteins
Colon
Breast
Endothelial Cells

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Kusuhara, M., Yamaguchi, K., Nagasaki, K., Hayashi, C., Suzaki, A., Hori, S., ... Abe, K. (1990). Production of endothelin in human cancer cell lines. Cancer Research, 50(11), 3257-3261.

Production of endothelin in human cancer cell lines. / Kusuhara, Masatoshi; Yamaguchi, Ken; Nagasaki, Koichi; Hayashi, Chiaki; Suzaki, Ai; Hori, Shingo; Handa, Shunnosuke; Nakamura, Yoshiro; Abe, Kaoru.

In: Cancer Research, Vol. 50, No. 11, 01.06.1990, p. 3257-3261.

Research output: Contribution to journalArticle

Kusuhara, M, Yamaguchi, K, Nagasaki, K, Hayashi, C, Suzaki, A, Hori, S, Handa, S, Nakamura, Y & Abe, K 1990, 'Production of endothelin in human cancer cell lines', Cancer Research, vol. 50, no. 11, pp. 3257-3261.
Kusuhara M, Yamaguchi K, Nagasaki K, Hayashi C, Suzaki A, Hori S et al. Production of endothelin in human cancer cell lines. Cancer Research. 1990 Jun 1;50(11):3257-3261.
Kusuhara, Masatoshi ; Yamaguchi, Ken ; Nagasaki, Koichi ; Hayashi, Chiaki ; Suzaki, Ai ; Hori, Shingo ; Handa, Shunnosuke ; Nakamura, Yoshiro ; Abe, Kaoru. / Production of endothelin in human cancer cell lines. In: Cancer Research. 1990 ; Vol. 50, No. 11. pp. 3257-3261.
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