Production of matrix metalloproteinases and tissue inhibitor of metalloproteinases-1 by human brain tumors

T. Nakagawa, T. Kubota, M. Kabuto, K. Sato, H. Kawano, T. Hayakawa, Y. Okada

Research output: Contribution to journalArticle

199 Citations (Scopus)

Abstract

The role of matrix metalloproteinases (MMP's) and their inhibitor, tissue inhibitor of metalloproteinases-1 (TIMP-1), in human brain tumor invasion was investigated. Gelatinolytic activity was assayed via gelatin zymography, and four MMP's (MMP-1, MMP-2, MMP-3, and MMP-9) and TIMP-1 were immunolocalized in human brain tumors and in normal brain tissues using monoclonal antibodies. The tissue was surgically removed from 44 patients: glioblastoma (five cases), anaplastic astrocytoma (six cases), astrocytoma (four cases), metastatic tumor (six cases), neurinoma (10 cases), meningioma (10 cases), and normal brain tissue (three cases). Glioblastomas, anaplastic astrocytomas, and metastatic tumors showed high gelatinolytic activity and positive immunostaining for MMP's; TIMP-1 was also expressed in these tumors, but some tumor cells were negative for the antibody. Astrocytomas had low gelatinolytic activity and the tumor cells showed no immunoreactivity for MMP's and TIMP-1. Although neurinomas and meningiomas had only moderate proteinase activity and exhibited positive immunoreactivity for MMP-9, intense expression of TIMP-1 was simultaneously observed in these tumor cells. These findings suggest that MMP's play an important role in human brain tumor invasion, probably due to an imbalance between the production of MMP's and TIMP-1 by the tumor cells.

Original languageEnglish
Pages (from-to)69-77
Number of pages9
JournalJournal of Neurosurgery
Volume81
Issue number1
Publication statusPublished - 1994

Fingerprint

Tissue Inhibitor of Metalloproteinase-1
Matrix Metalloproteinase Inhibitors
Brain Neoplasms
Astrocytoma
Matrix Metalloproteinases
Neoplasms
Neurilemmoma
Matrix Metalloproteinase 9
Meningioma
Glioblastoma
Matrix Metalloproteinase 3
Matrix Metalloproteinase 1
Matrix Metalloproteinase 2
Brain
Gelatin
Peptide Hydrolases
Monoclonal Antibodies
Antibodies

Keywords

  • brain neoplasm
  • matrix metalloproteinase
  • tissue inhibitor of metalloproteinases
  • tumor invasion

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Nakagawa, T., Kubota, T., Kabuto, M., Sato, K., Kawano, H., Hayakawa, T., & Okada, Y. (1994). Production of matrix metalloproteinases and tissue inhibitor of metalloproteinases-1 by human brain tumors. Journal of Neurosurgery, 81(1), 69-77.

Production of matrix metalloproteinases and tissue inhibitor of metalloproteinases-1 by human brain tumors. / Nakagawa, T.; Kubota, T.; Kabuto, M.; Sato, K.; Kawano, H.; Hayakawa, T.; Okada, Y.

In: Journal of Neurosurgery, Vol. 81, No. 1, 1994, p. 69-77.

Research output: Contribution to journalArticle

Nakagawa, T, Kubota, T, Kabuto, M, Sato, K, Kawano, H, Hayakawa, T & Okada, Y 1994, 'Production of matrix metalloproteinases and tissue inhibitor of metalloproteinases-1 by human brain tumors', Journal of Neurosurgery, vol. 81, no. 1, pp. 69-77.
Nakagawa T, Kubota T, Kabuto M, Sato K, Kawano H, Hayakawa T et al. Production of matrix metalloproteinases and tissue inhibitor of metalloproteinases-1 by human brain tumors. Journal of Neurosurgery. 1994;81(1):69-77.
Nakagawa, T. ; Kubota, T. ; Kabuto, M. ; Sato, K. ; Kawano, H. ; Hayakawa, T. ; Okada, Y. / Production of matrix metalloproteinases and tissue inhibitor of metalloproteinases-1 by human brain tumors. In: Journal of Neurosurgery. 1994 ; Vol. 81, No. 1. pp. 69-77.
@article{cbcdc189a07e429780032bbc4042642c,
title = "Production of matrix metalloproteinases and tissue inhibitor of metalloproteinases-1 by human brain tumors",
abstract = "The role of matrix metalloproteinases (MMP's) and their inhibitor, tissue inhibitor of metalloproteinases-1 (TIMP-1), in human brain tumor invasion was investigated. Gelatinolytic activity was assayed via gelatin zymography, and four MMP's (MMP-1, MMP-2, MMP-3, and MMP-9) and TIMP-1 were immunolocalized in human brain tumors and in normal brain tissues using monoclonal antibodies. The tissue was surgically removed from 44 patients: glioblastoma (five cases), anaplastic astrocytoma (six cases), astrocytoma (four cases), metastatic tumor (six cases), neurinoma (10 cases), meningioma (10 cases), and normal brain tissue (three cases). Glioblastomas, anaplastic astrocytomas, and metastatic tumors showed high gelatinolytic activity and positive immunostaining for MMP's; TIMP-1 was also expressed in these tumors, but some tumor cells were negative for the antibody. Astrocytomas had low gelatinolytic activity and the tumor cells showed no immunoreactivity for MMP's and TIMP-1. Although neurinomas and meningiomas had only moderate proteinase activity and exhibited positive immunoreactivity for MMP-9, intense expression of TIMP-1 was simultaneously observed in these tumor cells. These findings suggest that MMP's play an important role in human brain tumor invasion, probably due to an imbalance between the production of MMP's and TIMP-1 by the tumor cells.",
keywords = "brain neoplasm, matrix metalloproteinase, tissue inhibitor of metalloproteinases, tumor invasion",
author = "T. Nakagawa and T. Kubota and M. Kabuto and K. Sato and H. Kawano and T. Hayakawa and Y. Okada",
year = "1994",
language = "English",
volume = "81",
pages = "69--77",
journal = "Journal of Neurosurgery",
issn = "0022-3085",
publisher = "American Association of Neurological Surgeons",
number = "1",

}

TY - JOUR

T1 - Production of matrix metalloproteinases and tissue inhibitor of metalloproteinases-1 by human brain tumors

AU - Nakagawa, T.

AU - Kubota, T.

AU - Kabuto, M.

AU - Sato, K.

AU - Kawano, H.

AU - Hayakawa, T.

AU - Okada, Y.

PY - 1994

Y1 - 1994

N2 - The role of matrix metalloproteinases (MMP's) and their inhibitor, tissue inhibitor of metalloproteinases-1 (TIMP-1), in human brain tumor invasion was investigated. Gelatinolytic activity was assayed via gelatin zymography, and four MMP's (MMP-1, MMP-2, MMP-3, and MMP-9) and TIMP-1 were immunolocalized in human brain tumors and in normal brain tissues using monoclonal antibodies. The tissue was surgically removed from 44 patients: glioblastoma (five cases), anaplastic astrocytoma (six cases), astrocytoma (four cases), metastatic tumor (six cases), neurinoma (10 cases), meningioma (10 cases), and normal brain tissue (three cases). Glioblastomas, anaplastic astrocytomas, and metastatic tumors showed high gelatinolytic activity and positive immunostaining for MMP's; TIMP-1 was also expressed in these tumors, but some tumor cells were negative for the antibody. Astrocytomas had low gelatinolytic activity and the tumor cells showed no immunoreactivity for MMP's and TIMP-1. Although neurinomas and meningiomas had only moderate proteinase activity and exhibited positive immunoreactivity for MMP-9, intense expression of TIMP-1 was simultaneously observed in these tumor cells. These findings suggest that MMP's play an important role in human brain tumor invasion, probably due to an imbalance between the production of MMP's and TIMP-1 by the tumor cells.

AB - The role of matrix metalloproteinases (MMP's) and their inhibitor, tissue inhibitor of metalloproteinases-1 (TIMP-1), in human brain tumor invasion was investigated. Gelatinolytic activity was assayed via gelatin zymography, and four MMP's (MMP-1, MMP-2, MMP-3, and MMP-9) and TIMP-1 were immunolocalized in human brain tumors and in normal brain tissues using monoclonal antibodies. The tissue was surgically removed from 44 patients: glioblastoma (five cases), anaplastic astrocytoma (six cases), astrocytoma (four cases), metastatic tumor (six cases), neurinoma (10 cases), meningioma (10 cases), and normal brain tissue (three cases). Glioblastomas, anaplastic astrocytomas, and metastatic tumors showed high gelatinolytic activity and positive immunostaining for MMP's; TIMP-1 was also expressed in these tumors, but some tumor cells were negative for the antibody. Astrocytomas had low gelatinolytic activity and the tumor cells showed no immunoreactivity for MMP's and TIMP-1. Although neurinomas and meningiomas had only moderate proteinase activity and exhibited positive immunoreactivity for MMP-9, intense expression of TIMP-1 was simultaneously observed in these tumor cells. These findings suggest that MMP's play an important role in human brain tumor invasion, probably due to an imbalance between the production of MMP's and TIMP-1 by the tumor cells.

KW - brain neoplasm

KW - matrix metalloproteinase

KW - tissue inhibitor of metalloproteinases

KW - tumor invasion

UR - http://www.scopus.com/inward/record.url?scp=0028282875&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028282875&partnerID=8YFLogxK

M3 - Article

C2 - 8207529

AN - SCOPUS:0028282875

VL - 81

SP - 69

EP - 77

JO - Journal of Neurosurgery

JF - Journal of Neurosurgery

SN - 0022-3085

IS - 1

ER -