Production of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human breast carcinomas

Hiroji Iwata, Shunzo Kobayashi, Hirotaka Iwase, Akira Masaoka, Noboru Fujimoto, Yasunori Okada

Research output: Contribution to journalArticle

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Abstract

We examined production and tissue localization of matrix metalloproteinase (MMP)-1 (tissue collagenase), MMP-2 (gelatinase A), MMP-3 (stromelysin-1), MMP-9 (gelatinase B), tissue inhibitors of metalloproteinase (TIMP)-1 and TIMP-2 in human breast carcinomas. In more than half of the cases, MMP-1, MMP-2, MMP-9, TIMP-1 and TIMP-2 were immunolocalized in carcinoma cells and MMP-2 was on the carcinoma cell membranes as well, whereas MMP-3 was positively stained in less than 15% of the cases. MMP-1 staining in carcinoma cells was significantly higher In scirrhous carcinoma than in other types of carcinoma. MMP-9 expression was remarkably higher in the carcinoma cases with lymphnode metastasis than in the non-metastatic cases. MMP-3 was mainly expressed in T-lymphocytes infiltrated in the tumor stroma. Stromal fibroblasts were positive for all these MMPs except for MMP-3. The TIMP-1 levels released into the culture media by carcinoma tissues were significantly lower than those by fibroadenoma tissues, although there were no significant differences in the levels of MMP-1, MMP-2, MMP-9 and TIMP-2. Gelatin zymographical analyses showed that the activation rate of the zymogen of MMP-2 (proMMP-2) is significantly higher in the more advanced carcinoma group with lymphnode metastasis than in the metastasis-negative and fibroadenoma groups. These data indicate that MMP-1, MMP-2 and MMP-9 are highly expressed in human breast carcinoma tissue and suggest that activation of proMMP-2 may be an indicator of lymphnode metastasis of the breast carcinoma.

Original languageEnglish
Pages (from-to)602-611
Number of pages10
JournalJapanese Journal of Cancer Research
Volume87
Issue number6
Publication statusPublished - 1996 Jun

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Tissue Inhibitor of Metalloproteinases
Matrix Metalloproteinase Inhibitors
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Matrix Metalloproteinase 3
Matrix Metalloproteinase 1
Breast Neoplasms
Carcinoma
Tissue Inhibitor of Metalloproteinase-1
Neoplasm Metastasis
Fibroadenoma
Tissue Inhibitor of Metalloproteinase-2
Scirrhous Adenocarcinoma
Activation Analysis
Enzyme Precursors
Collagenases
Gelatin
Matrix Metalloproteinases
Culture Media
Fibroblasts

Keywords

  • Breast cancer
  • Cancer invasion
  • Matrix metalloproteinase
  • Metastasis
  • Tissue inhibitor of metalloproteinase

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Iwata, H., Kobayashi, S., Iwase, H., Masaoka, A., Fujimoto, N., & Okada, Y. (1996). Production of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human breast carcinomas. Japanese Journal of Cancer Research, 87(6), 602-611.

Production of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human breast carcinomas. / Iwata, Hiroji; Kobayashi, Shunzo; Iwase, Hirotaka; Masaoka, Akira; Fujimoto, Noboru; Okada, Yasunori.

In: Japanese Journal of Cancer Research, Vol. 87, No. 6, 06.1996, p. 602-611.

Research output: Contribution to journalArticle

Iwata, H, Kobayashi, S, Iwase, H, Masaoka, A, Fujimoto, N & Okada, Y 1996, 'Production of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human breast carcinomas', Japanese Journal of Cancer Research, vol. 87, no. 6, pp. 602-611.
Iwata, Hiroji ; Kobayashi, Shunzo ; Iwase, Hirotaka ; Masaoka, Akira ; Fujimoto, Noboru ; Okada, Yasunori. / Production of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human breast carcinomas. In: Japanese Journal of Cancer Research. 1996 ; Vol. 87, No. 6. pp. 602-611.
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