Production of recombinant extracellular domains of canine desmoglein 1 (Dsg1) by baculovirus expression

Koji Nishifuji; Masayuki Amagai; Takeji Nishikawa; Toshiroh Iwasaki

doi:10.1016/S0165-2427(03)00107-7

Production of recombinant extracellular domains of canine desmoglein 1 (Dsg1) by baculovirus expression

Koji Nishifuji, Masayuki Amagai, Takeji Nishikawa, Toshiroh Iwasaki

Department of Dermatology

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

The aim of this study was to generate a recombinant protein to represent the entire extracellular domain of canine desmoglein 1 (Dsg1), a desmosomal cell-cell adhesion molecule, by the baculovirus expression system. Cotransfection of a baculovirus transfer vector containing the cDNA for the entire extracellular domain of canine Dsg1 with baculovirus DNA into insect cells resulted in the secretion of soluble canine Dsg1 into insect culture supernatants. Immunoreactivity of 11 human pemphigus foliaceus (PF) sera against the cell surface of canine keratinocytes was completely removed when the sera were preincubated with the canine Dsg1 baculoprotein. This recombinant canine Dsg1 produced by baculovirus shares the major epitopes of the authentic canine Dsg1 recognized by human PF sera, and will be useful in studying the molecular pathophysiological mechanisms in PF and impetigo in canine patients.

Original language	English
Pages (from-to)	177-182
Number of pages	6
Journal	Veterinary Immunology and Immunopathology
Volume	95
Issue number	3-4
DOIs	https://doi.org/10.1016/S0165-2427(03)00107-7
Publication status	Published - 2003 Oct 15

Keywords

Baculovirus expression
Desmoglein
Dog
Impetigo
Pemphigus

ASJC Scopus subject areas

Immunology
veterinary(all)

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title = "Production of recombinant extracellular domains of canine desmoglein 1 (Dsg1) by baculovirus expression",

abstract = "The aim of this study was to generate a recombinant protein to represent the entire extracellular domain of canine desmoglein 1 (Dsg1), a desmosomal cell-cell adhesion molecule, by the baculovirus expression system. Cotransfection of a baculovirus transfer vector containing the cDNA for the entire extracellular domain of canine Dsg1 with baculovirus DNA into insect cells resulted in the secretion of soluble canine Dsg1 into insect culture supernatants. Immunoreactivity of 11 human pemphigus foliaceus (PF) sera against the cell surface of canine keratinocytes was completely removed when the sera were preincubated with the canine Dsg1 baculoprotein. This recombinant canine Dsg1 produced by baculovirus shares the major epitopes of the authentic canine Dsg1 recognized by human PF sera, and will be useful in studying the molecular pathophysiological mechanisms in PF and impetigo in canine patients.",

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AU - Amagai, Masayuki

AU - Nishikawa, Takeji

AU - Iwasaki, Toshiroh

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AB - The aim of this study was to generate a recombinant protein to represent the entire extracellular domain of canine desmoglein 1 (Dsg1), a desmosomal cell-cell adhesion molecule, by the baculovirus expression system. Cotransfection of a baculovirus transfer vector containing the cDNA for the entire extracellular domain of canine Dsg1 with baculovirus DNA into insect cells resulted in the secretion of soluble canine Dsg1 into insect culture supernatants. Immunoreactivity of 11 human pemphigus foliaceus (PF) sera against the cell surface of canine keratinocytes was completely removed when the sera were preincubated with the canine Dsg1 baculoprotein. This recombinant canine Dsg1 produced by baculovirus shares the major epitopes of the authentic canine Dsg1 recognized by human PF sera, and will be useful in studying the molecular pathophysiological mechanisms in PF and impetigo in canine patients.

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