Abstract
The aim of this study was to generate a recombinant protein to represent the entire extracellular domain of canine desmoglein 1 (Dsg1), a desmosomal cell-cell adhesion molecule, by the baculovirus expression system. Cotransfection of a baculovirus transfer vector containing the cDNA for the entire extracellular domain of canine Dsg1 with baculovirus DNA into insect cells resulted in the secretion of soluble canine Dsg1 into insect culture supernatants. Immunoreactivity of 11 human pemphigus foliaceus (PF) sera against the cell surface of canine keratinocytes was completely removed when the sera were preincubated with the canine Dsg1 baculoprotein. This recombinant canine Dsg1 produced by baculovirus shares the major epitopes of the authentic canine Dsg1 recognized by human PF sera, and will be useful in studying the molecular pathophysiological mechanisms in PF and impetigo in canine patients.
| Original language | English |
|---|---|
| Pages (from-to) | 177-182 |
| Number of pages | 6 |
| Journal | Veterinary Immunology and Immunopathology |
| Volume | 95 |
| Issue number | 3-4 |
| DOIs | |
| Publication status | Published - 2003 Oct 15 |
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Keywords
- Baculovirus expression
- Desmoglein
- Dog
- Impetigo
- Pemphigus
ASJC Scopus subject areas
- Animal Science and Zoology
- Immunology
- veterinary(all)
Cite this
Production of recombinant extracellular domains of canine desmoglein 1 (Dsg1) by baculovirus expression. / Nishifuji, Koji; Amagai, Masayuki; Nishikawa, Takeji; Iwasaki, Toshiroh.
In: Veterinary Immunology and Immunopathology, Vol. 95, No. 3-4, 15.10.2003, p. 177-182.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Production of recombinant extracellular domains of canine desmoglein 1 (Dsg1) by baculovirus expression
AU - Nishifuji, Koji
AU - Amagai, Masayuki
AU - Nishikawa, Takeji
AU - Iwasaki, Toshiroh
PY - 2003/10/15
Y1 - 2003/10/15
N2 - The aim of this study was to generate a recombinant protein to represent the entire extracellular domain of canine desmoglein 1 (Dsg1), a desmosomal cell-cell adhesion molecule, by the baculovirus expression system. Cotransfection of a baculovirus transfer vector containing the cDNA for the entire extracellular domain of canine Dsg1 with baculovirus DNA into insect cells resulted in the secretion of soluble canine Dsg1 into insect culture supernatants. Immunoreactivity of 11 human pemphigus foliaceus (PF) sera against the cell surface of canine keratinocytes was completely removed when the sera were preincubated with the canine Dsg1 baculoprotein. This recombinant canine Dsg1 produced by baculovirus shares the major epitopes of the authentic canine Dsg1 recognized by human PF sera, and will be useful in studying the molecular pathophysiological mechanisms in PF and impetigo in canine patients.
AB - The aim of this study was to generate a recombinant protein to represent the entire extracellular domain of canine desmoglein 1 (Dsg1), a desmosomal cell-cell adhesion molecule, by the baculovirus expression system. Cotransfection of a baculovirus transfer vector containing the cDNA for the entire extracellular domain of canine Dsg1 with baculovirus DNA into insect cells resulted in the secretion of soluble canine Dsg1 into insect culture supernatants. Immunoreactivity of 11 human pemphigus foliaceus (PF) sera against the cell surface of canine keratinocytes was completely removed when the sera were preincubated with the canine Dsg1 baculoprotein. This recombinant canine Dsg1 produced by baculovirus shares the major epitopes of the authentic canine Dsg1 recognized by human PF sera, and will be useful in studying the molecular pathophysiological mechanisms in PF and impetigo in canine patients.
KW - Baculovirus expression
KW - Desmoglein
KW - Dog
KW - Impetigo
KW - Pemphigus
UR - http://www.scopus.com/inward/record.url?scp=0043237961&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0043237961&partnerID=8YFLogxK
U2 - 10.1016/S0165-2427(03)00107-7
DO - 10.1016/S0165-2427(03)00107-7
M3 - Article
VL - 95
SP - 177
EP - 182
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
SN - 0165-2427
IS - 3-4
ER -