Production of small noncoding RNAs from the flamenco locus is regulated by the gypsy retrotransposon of Drosophila melanogaster

Vincenzo Guida, Filippo M. Cernilogar, Angela Filograna, Roberto De Gregorio, Hirotsugu Ishizu, Mikiko C. Siomi, Gunnar Schotta, Gian Carlo Bellenchi, Davide Andrenacci

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Protective mechanisms based on RNA silencing directed against the propagation of transposable elements are highly conserved in eukaryotes. The control of transposable elements is mediated by small noncoding RNAs, which derive from transposon-rich heterochromatic regions that function as small RNA-generating loci. These clusters are transcribed and the precursor transcripts are processed to generate Piwi-interacting RNAs (piRNAs) and endogenous small interfering RNAs (endo-siRNAs), which silence transposable elements in gonads and somatic tissues. The flamenco locus is a Drosophila melanogaster small RNA cluster that controls gypsy and other transposable elements, and has played an important role in understanding how small noncoding RNAs repress transposable elements. In this study, we describe a cosuppression mechanism triggered by new euchromatic gypsy insertions in genetic backgrounds carrying flamenco alleles defective in gypsy suppression. We found that the silencing of gypsy is accompanied by the silencing of other transposons regulated by flamenco, and of specific flamenco sequences from which small RNAs against gypsy originate. This cosuppression mechanism seems to depend on a post-transcriptional regulation that involves both endo-siRNA and piRNA pathways and is associated with the occurrence of developmental defects. In conclusion, we propose that new gypsy euchromatic insertions trigger a post-transcriptional silencing of gypsy sense and antisense sequences, which modifies the flamenco activity. This cosuppression mechanism interferes with some developmental processes, presumably by influencing the expression of specific genes.

Original languageEnglish
Pages (from-to)631-644
Number of pages14
JournalGenetics
Volume204
Issue number2
DOIs
Publication statusPublished - 2016 Oct 1
Externally publishedYes

Fingerprint

Small Untranslated RNA
Retroelements
DNA Transposable Elements
Drosophila melanogaster
RNA Interference
Small Interfering RNA
RNA
Gonads
Eukaryota
Alleles
Gene Expression

Keywords

  • Ecdysis
  • Primary transcript
  • RNA silencing
  • Small RNA
  • Transposon

ASJC Scopus subject areas

  • Genetics

Cite this

Production of small noncoding RNAs from the flamenco locus is regulated by the gypsy retrotransposon of Drosophila melanogaster. / Guida, Vincenzo; Cernilogar, Filippo M.; Filograna, Angela; De Gregorio, Roberto; Ishizu, Hirotsugu; Siomi, Mikiko C.; Schotta, Gunnar; Bellenchi, Gian Carlo; Andrenacci, Davide.

In: Genetics, Vol. 204, No. 2, 01.10.2016, p. 631-644.

Research output: Contribution to journalArticle

Guida, V, Cernilogar, FM, Filograna, A, De Gregorio, R, Ishizu, H, Siomi, MC, Schotta, G, Bellenchi, GC & Andrenacci, D 2016, 'Production of small noncoding RNAs from the flamenco locus is regulated by the gypsy retrotransposon of Drosophila melanogaster', Genetics, vol. 204, no. 2, pp. 631-644. https://doi.org/10.1534/genetics.116.187922
Guida, Vincenzo ; Cernilogar, Filippo M. ; Filograna, Angela ; De Gregorio, Roberto ; Ishizu, Hirotsugu ; Siomi, Mikiko C. ; Schotta, Gunnar ; Bellenchi, Gian Carlo ; Andrenacci, Davide. / Production of small noncoding RNAs from the flamenco locus is regulated by the gypsy retrotransposon of Drosophila melanogaster. In: Genetics. 2016 ; Vol. 204, No. 2. pp. 631-644.
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