Products of the reaction between α- or γ-tocopherol and nitrogen oxides analyzed by high-performance liquid chromatography with UV-visible and atmospheric pressure chemical ionization mass spectrometric detection

Yoshiko Itoh, Yohta Matsumoto, Hideko Kanazawa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The reaction products of α- or γ-tocopherol with nitric oxide in the presence of molecular oxygen were isolated and characterized. The consumption of tocopherols and the formation of the major products were monitored by high-performance liquid chromatography (HPLC) by a gradient elution method. The quantitative analysis of these compounds with UV-Vis detectors, however, was interfered by several minor products having similar UV spectra and retention times as those of the major ones. In order to establish a quantitative analytical method for the products, we investigated other detection methods, and found that atmospheric pressure chemical ionization (APCI), LC-MS was a more selective and better analytical method for these compounds.

Original languageEnglish
Pages (from-to)177-182
Number of pages6
JournalJournal of Chromatography A
Volume1036
Issue number2
DOIs
Publication statusPublished - 2004 May 21

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Nitrogen Oxides
Atmospheric Pressure
Tocopherols
High performance liquid chromatography
Atmospheric pressure
Ionization
High Pressure Liquid Chromatography
Ultraviolet detectors
Gradient methods
Molecular oxygen
Reaction products
Nitric Oxide
Chemical analysis
Oxygen

Keywords

  • Nitrogen oxides
  • Tocopherols

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

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abstract = "The reaction products of α- or γ-tocopherol with nitric oxide in the presence of molecular oxygen were isolated and characterized. The consumption of tocopherols and the formation of the major products were monitored by high-performance liquid chromatography (HPLC) by a gradient elution method. The quantitative analysis of these compounds with UV-Vis detectors, however, was interfered by several minor products having similar UV spectra and retention times as those of the major ones. In order to establish a quantitative analytical method for the products, we investigated other detection methods, and found that atmospheric pressure chemical ionization (APCI), LC-MS was a more selective and better analytical method for these compounds.",
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T1 - Products of the reaction between α- or γ-tocopherol and nitrogen oxides analyzed by high-performance liquid chromatography with UV-visible and atmospheric pressure chemical ionization mass spectrometric detection

AU - Itoh, Yoshiko

AU - Matsumoto, Yohta

AU - Kanazawa, Hideko

PY - 2004/5/21

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AB - The reaction products of α- or γ-tocopherol with nitric oxide in the presence of molecular oxygen were isolated and characterized. The consumption of tocopherols and the formation of the major products were monitored by high-performance liquid chromatography (HPLC) by a gradient elution method. The quantitative analysis of these compounds with UV-Vis detectors, however, was interfered by several minor products having similar UV spectra and retention times as those of the major ones. In order to establish a quantitative analytical method for the products, we investigated other detection methods, and found that atmospheric pressure chemical ionization (APCI), LC-MS was a more selective and better analytical method for these compounds.

KW - Nitrogen oxides

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