Profiling of the charged metabolites of traditional herbal medicines using capillary electrophoresis time-of-flight mass spectrometry

Keiko Iino, Masahiro Sugimoto, Tomoyoshi Soga, Masaru Tomita

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The quantification of a small number of bioactive components in herbal medicines is often inadequate when attempting to elucidate a medicine's biological effects. Despite rapid advances in analytical technologies, obtaining comprehensive metabolomic profiles of herbal medicines remains difficult, due to the complexity of natural product mixtures. Toki-Shakuyaku-San is a Chinese medicine used widely to treat gynecological and obstetric disorders, such as infertility, dysmenorrhea, toxemia during pregnancy and neural dysfunction. It consists of Angelica acutiloba Radix (Toki), Cnidium officinale Rhizoma (Senkyu), Paeonia lactiflora Radix (Shakuyaku), Atractylodes lancea Rhizoma (Sojutsu), Alisma orientale Rhizoma (Takusha) and Poria cocos Hoelen (Bukuryo). To elucidate the composition of these herbal medicines individually, we conducted non-targeted profiling analyses of extracts of these herbs using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS), which allows the simultaneous quantification of hundreds of charged metabolites. In total, 737 ± 183. 1 (average ± SD) metabolite-derived features were observed, and of these, 119 metabolites were identified. Score plots of principal component analysis (PCA) showed a clear cluster including Shakuyaku, Bukuryo, and Sojutsu, while the other three herbs were distributed over PCA spaces. Loading plots revealed that amino acids and shikimate-derived alkaloids were the predominant metabolite constituents. Hierarchical clustering analysis revealed that few clusters overlapped in the herbal medicines tested. This report is the first demonstration of the characterization of a herbal medicine using large-scale metabolomic analysis, which is complementary to traditional quality control methods.

Original languageEnglish
Pages (from-to)99-108
Number of pages10
JournalMetabolomics
Volume8
Issue number1
DOIs
Publication statusPublished - 2012 Feb

Fingerprint

Capillary electrophoresis
Herbal Medicine
Capillary Electrophoresis
Traditional Medicine
Metabolites
Mass spectrometry
Paeonia
Mass Spectrometry
Metabolomics
Principal component analysis
Medicine
Principal Component Analysis
Obstetrics
Alisma
Cnidium
Atractylodes
Angelica
Biological Products
Dysmenorrhea
Alkaloids

Keywords

  • Capillary electrophoresis time-of-flight mass spectrometry
  • Charged metabolite
  • Herbal medicine
  • Metabolomic profiling

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Profiling of the charged metabolites of traditional herbal medicines using capillary electrophoresis time-of-flight mass spectrometry. / Iino, Keiko; Sugimoto, Masahiro; Soga, Tomoyoshi; Tomita, Masaru.

In: Metabolomics, Vol. 8, No. 1, 02.2012, p. 99-108.

Research output: Contribution to journalArticle

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