Profiling open chromatin structure in the ovarian somatic cells using ATAC-seq

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The assay for transposase-accessible chromatin using sequencing (ATAC-seq) was recently established as a method to profile open chromatin, which overcomes the sample size limitations of the alternative methods DNase/MNase-seq. To investigate the role of Piwi in heterochromatin formation around transposable element loci, we have used ATAC-seq to examine chromatin accessibility at target transposable elements in a Drosophila cultured cell line, ovarian somatic cells (OSCs). In this chapter, we describe our method to profile open chromatin structure in OSCs using ATAC-seq.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages165-177
Number of pages13
Volume1680
DOIs
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1680
ISSN (Print)1064-3745

Keywords

  • ATAC-seq
  • Chromatin accessibility
  • Drosophila
  • Heterochromatin
  • OSC
  • Small RNA
  • Transposons

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Murano, K., Iwasaki, Y., & Siomi, H. (2018). Profiling open chromatin structure in the ovarian somatic cells using ATAC-seq. In Methods in Molecular Biology (Vol. 1680, pp. 165-177). (Methods in Molecular Biology; Vol. 1680). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7339-2_11