Prognostic significance of interleukin-8 and CD163-positive cell-infiltration in tumor tissues in patients with oral squamous cell carcinoma

Yohei Fujita, Masato Okamoto, Hiroyuki Goda, Tomoyuki Tano, Koh Ichi Nakashiro, Atsuro Sugita, Tomonobu Fujita, Shigeo Koido, Sadamu Homma, Yutaka Kawakami, Hiroyuki Hamakawa

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37 Citations (Scopus)

Abstract

Purpose: We investigated whether serum interleukin (IL)-8 reflects the tumor microenvironment and has prognostic value in patients with oral squamous cell carcinoma (OSCC). Experimental Design: Fifty OSCC patients who received radical resection of their tumor(s) were enrolled. Preoperative sera were measured for IL-8 by ELISA. Expression of IL-8 and the infiltration of immune cells in tumor tissues were analyzed by an immunohistochemical staining of surgical specimens. Results: We found that disease-free survival (DFS) was significantly longer in the Stage I/II OSCC patients with low serum IL-8 levels compared to those with high levels (p=0.001). The tumor expression of IL-8, i.e., IL-8(T) and the density of CD163-positive cells in the tumor invasive front, i.e., CD163(IF) were correlated with the serum IL-8 level (p =0.033 and p=0.038, respectively), and they were associated with poor clinical outcome (p= 0.007 and p=0.002, respectively, in DFS) in all patients. A multivariate analysis revealed that N status, IL-8(T) and CD163(IF) significantly affected the DFS of the patients. Further analysis suggested that combination of N status with serum IL-8, IL-8(T) or CD163(IF) may be a new criterion for discriminating between OSCC patients at high and low risk for tumor relapse. Interestingly, the in vitro experiments demonstrated that IL-8 enhanced generation of CD163-positive M2 macrophages from peripheral blood monocytes, and that the cells produced IL-10. Conclusions: These findings indicate that IL-8 may be involved in poor clinical outcomes via generation of CD163-positive M2 macrophages, and that these factors in addition to N status may have prognostic value in patients with resectable OSCSS.

Original languageEnglish
Article numbere110378
JournalPloS one
Volume9
Issue number12
DOIs
Publication statusPublished - 2014 Dec 2

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Fujita, Y., Okamoto, M., Goda, H., Tano, T., Nakashiro, K. I., Sugita, A., Fujita, T., Koido, S., Homma, S., Kawakami, Y., & Hamakawa, H. (2014). Prognostic significance of interleukin-8 and CD163-positive cell-infiltration in tumor tissues in patients with oral squamous cell carcinoma. PloS one, 9(12), [e110378]. https://doi.org/10.1371/journal.pone.0110378