Prognostic significance of laminin-5 γ2 chain expression in colorectal carcinoma

Immunohistochemical analysis of 103 cases

Seishi Aoki, Yukihiro Nakanishi, Shingo Akimoto, Yoshihiro Moriya, Kimio Yoshimura, Masaki Kitajima, Michiie Sakamoto, Setsuo Hirohashi

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

PURPOSE: The laminin-5 γ2 chain plays an important role in cell migration during tumor invasion and tissue remodeling. Although this chain has been reported to be expressed in tumor-stroma interface of colorectal carcinoma, prognostic significance of its expression has not been elucidated in these tumors, so we investigated the clinicopathologic significance of Laminin-5 γ2 chain expression in colorectal carcinoma. METHODS: Laminin-5 γ2 chain expression was investigated immunohistochemically in 103 colorectal carcinoma patients with Stage II, III, and IV disease. The patients were categorized into three groups according to the number of immunopositive tumor cells in the sections containing the maximum diameter of the tumor as follows: +, less than 20 tumor cells were positive; ++, 20 to 500 tumor cells were positive; +++, more than 500 tumor cells were positive. RESULTS: Laminin-5 γ2 chain expression was observed in cytoplasm of tumor cells, especially those in the invasive front of the tumor penetration. Eighteen (17 percent) of tumors showed +, 60 (58 percent) showed ++, and 25 (24 percent) showed +++. The increased number of immunopositive tumor cells was significantly associated with synchronous liver metastasis (P = 0.048). The univariate (P = 0.036) and multivariate (P = 0.001) analysis of the patients' survival revealed that the prognosis became significantly poorer in patients with the increased number of immunopositive tumor cells. CONCLUSIONS: Increased laminin-5 γ2 chain immunoreactivity, suggesting a high invasive potential of tumor cells, was a significant poor prognostic indicator for the patients with colorectal carcinoma.

Original languageEnglish
Pages (from-to)1520-1527
Number of pages8
JournalDiseases of the Colon and Rectum
Volume45
Issue number11
DOIs
Publication statusPublished - 2002 Nov 1
Externally publishedYes

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Colorectal Neoplasms
Neoplasms
kalinin
Survival Analysis
Cell Movement
Cytoplasm
Neoplasm Metastasis

Keywords

  • Colorectal carcinoma
  • Immunohistochemistry
  • Laminin-5 γ2 chain
  • Prognosis

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Prognostic significance of laminin-5 γ2 chain expression in colorectal carcinoma : Immunohistochemical analysis of 103 cases. / Aoki, Seishi; Nakanishi, Yukihiro; Akimoto, Shingo; Moriya, Yoshihiro; Yoshimura, Kimio; Kitajima, Masaki; Sakamoto, Michiie; Hirohashi, Setsuo.

In: Diseases of the Colon and Rectum, Vol. 45, No. 11, 01.11.2002, p. 1520-1527.

Research output: Contribution to journalArticle

Aoki, Seishi ; Nakanishi, Yukihiro ; Akimoto, Shingo ; Moriya, Yoshihiro ; Yoshimura, Kimio ; Kitajima, Masaki ; Sakamoto, Michiie ; Hirohashi, Setsuo. / Prognostic significance of laminin-5 γ2 chain expression in colorectal carcinoma : Immunohistochemical analysis of 103 cases. In: Diseases of the Colon and Rectum. 2002 ; Vol. 45, No. 11. pp. 1520-1527.
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T1 - Prognostic significance of laminin-5 γ2 chain expression in colorectal carcinoma

T2 - Immunohistochemical analysis of 103 cases

AU - Aoki, Seishi

AU - Nakanishi, Yukihiro

AU - Akimoto, Shingo

AU - Moriya, Yoshihiro

AU - Yoshimura, Kimio

AU - Kitajima, Masaki

AU - Sakamoto, Michiie

AU - Hirohashi, Setsuo

PY - 2002/11/1

Y1 - 2002/11/1

N2 - PURPOSE: The laminin-5 γ2 chain plays an important role in cell migration during tumor invasion and tissue remodeling. Although this chain has been reported to be expressed in tumor-stroma interface of colorectal carcinoma, prognostic significance of its expression has not been elucidated in these tumors, so we investigated the clinicopathologic significance of Laminin-5 γ2 chain expression in colorectal carcinoma. METHODS: Laminin-5 γ2 chain expression was investigated immunohistochemically in 103 colorectal carcinoma patients with Stage II, III, and IV disease. The patients were categorized into three groups according to the number of immunopositive tumor cells in the sections containing the maximum diameter of the tumor as follows: +, less than 20 tumor cells were positive; ++, 20 to 500 tumor cells were positive; +++, more than 500 tumor cells were positive. RESULTS: Laminin-5 γ2 chain expression was observed in cytoplasm of tumor cells, especially those in the invasive front of the tumor penetration. Eighteen (17 percent) of tumors showed +, 60 (58 percent) showed ++, and 25 (24 percent) showed +++. The increased number of immunopositive tumor cells was significantly associated with synchronous liver metastasis (P = 0.048). The univariate (P = 0.036) and multivariate (P = 0.001) analysis of the patients' survival revealed that the prognosis became significantly poorer in patients with the increased number of immunopositive tumor cells. CONCLUSIONS: Increased laminin-5 γ2 chain immunoreactivity, suggesting a high invasive potential of tumor cells, was a significant poor prognostic indicator for the patients with colorectal carcinoma.

AB - PURPOSE: The laminin-5 γ2 chain plays an important role in cell migration during tumor invasion and tissue remodeling. Although this chain has been reported to be expressed in tumor-stroma interface of colorectal carcinoma, prognostic significance of its expression has not been elucidated in these tumors, so we investigated the clinicopathologic significance of Laminin-5 γ2 chain expression in colorectal carcinoma. METHODS: Laminin-5 γ2 chain expression was investigated immunohistochemically in 103 colorectal carcinoma patients with Stage II, III, and IV disease. The patients were categorized into three groups according to the number of immunopositive tumor cells in the sections containing the maximum diameter of the tumor as follows: +, less than 20 tumor cells were positive; ++, 20 to 500 tumor cells were positive; +++, more than 500 tumor cells were positive. RESULTS: Laminin-5 γ2 chain expression was observed in cytoplasm of tumor cells, especially those in the invasive front of the tumor penetration. Eighteen (17 percent) of tumors showed +, 60 (58 percent) showed ++, and 25 (24 percent) showed +++. The increased number of immunopositive tumor cells was significantly associated with synchronous liver metastasis (P = 0.048). The univariate (P = 0.036) and multivariate (P = 0.001) analysis of the patients' survival revealed that the prognosis became significantly poorer in patients with the increased number of immunopositive tumor cells. CONCLUSIONS: Increased laminin-5 γ2 chain immunoreactivity, suggesting a high invasive potential of tumor cells, was a significant poor prognostic indicator for the patients with colorectal carcinoma.

KW - Colorectal carcinoma

KW - Immunohistochemistry

KW - Laminin-5 γ2 chain

KW - Prognosis

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