Progressive multifocal leukoencephalopathy (PML) -Report of a case diagnosed by amplification of JC virus DNA from cerebrospinal fluid (CSF)

Daisuke Ito, Yoshiaki Yogo, Hajime Okita, Kortaro Tanaka, Atsuo Koto

Research output: Contribution to journalArticle

Abstract

We report a 54-year-old Japanese man who was diagnosed as progressive multifocal leukoencephalopathy (PML). He had been maintained on regular hemodialysis for 10 years. He was admitted to our hospital with chief complaints of visual disturbance and disorientation. On neurological examination, he was somnolent, and showed mild weakness in the right upper and lower limbs. Deep tendon reflexes were brisk on the right upper and bilateral lower limbs. Sensory examination revealed no abnormal findings. The cerebrospinal fluid (CSF) showed five mononuclear cells /mm3, protein 39 mg/dl and glucose 38 mg/dl. Brain MRI revealed multiple hyperintense lesions in T2-images, which were confined to the white matter of bilateral occipital and the left frontal lobes without an enhancement after gadolinium administration. Using polymerase chain reaction, we amplified the JCV regulatory region from the CSF of the patient. The amplified product contained a rearranged regulatory region that could have been generated from the archetype by deletion and amplification. PML was diagnosed on the basis of these findings. The patient died 7 months after onset of the symptoms. The diagnosis of PML was confirmed by the postmortem findings. The present case indicates that PCR of JCV from CSF is very useful for definitive diagnosis of PML.

Original languageEnglish
Pages (from-to)46-50
Number of pages5
JournalClinical Neurology
Volume38
Issue number1
Publication statusPublished - 1998 Jan

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JC Virus
Progressive Multifocal Leukoencephalopathy
Cerebrospinal Fluid
Nucleic Acid Regulatory Sequences
DNA
Lower Extremity
Stretch Reflex
Polymerase Chain Reaction
Neurologic Examination
Gadolinium
Frontal Lobe
Renal Dialysis
Glucose
Brain
Proteins

Keywords

  • Cerebrospinal fluid
  • Hemodialysis
  • JC virus
  • Polymerase chain reaction
  • Progressive multifocal leukoencephalopathy

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Progressive multifocal leukoencephalopathy (PML) -Report of a case diagnosed by amplification of JC virus DNA from cerebrospinal fluid (CSF). / Ito, Daisuke; Yogo, Yoshiaki; Okita, Hajime; Tanaka, Kortaro; Koto, Atsuo.

In: Clinical Neurology, Vol. 38, No. 1, 01.1998, p. 46-50.

Research output: Contribution to journalArticle

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