Proliferation and adhesion of L929 fibroblasts on surface with different microtopography

Yuta Kurashina, Shogo Miyata, Jun Komotori, Tadayoshi Koyama

Research output: Contribution to journalArticle

Abstract

Three types of 316L stainless steel surface with different topography were prepared by a Fine Particle Peening (FPP) treatment using titania, silica and alumina shot particles and analyzed the cell proliferation and cell-scaffold interaction. FPP-treated surface with titania and silica particles had micro asperities at low frequency. On the other hand, the alumina treated surface had micro asperities at high frequency. L929 fibroblasts were seeded on these specimens and then the number of cells was counted after 72 hours of culturing. The FPP-treated surfaces showed good cell proliferation comparing to polished surface. This indicates that micro asperities formed on the surface encourage cell adhesion. Cell adhesion behavior was evaluated by a scanning electron microscope (SEM) and a fluorescence microscope. Dense filopodia were observed when cells cultured on the FPP-treated surface. This means that FPP treatment enhances cell adhesion and proliferation. The number of cells observed on the FPP-treated surface depended on the shape of asperities formed by FPP treatment; the highest cell counts were obtained on alumina treated surface. This is because cell migration was not inhibited by the shape of alumina treated surface asperities.

Original languageEnglish
JournalPrehospital and Disaster Medicine
Volume1648
Issue number4
DOIs
Publication statusPublished - 2014 Nov 17

Fingerprint

Aluminum Oxide
Fibroblasts
Cell Adhesion
Cell Count
Cell Proliferation
Silicon Dioxide
Pseudopodia
Stainless Steel
Cell Communication
Cell Movement
Cultured Cells
Fluorescence
Electrons
titanium dioxide

Keywords

  • biomaterial
  • biomedical
  • metal

ASJC Scopus subject areas

  • Emergency
  • Emergency Medicine

Cite this

Proliferation and adhesion of L929 fibroblasts on surface with different microtopography. / Kurashina, Yuta; Miyata, Shogo; Komotori, Jun; Koyama, Tadayoshi.

In: Prehospital and Disaster Medicine, Vol. 1648, No. 4, 17.11.2014.

Research output: Contribution to journalArticle

@article{c7205aca6d1a41d3b30504f7cd26e2bc,
title = "Proliferation and adhesion of L929 fibroblasts on surface with different microtopography",
abstract = "Three types of 316L stainless steel surface with different topography were prepared by a Fine Particle Peening (FPP) treatment using titania, silica and alumina shot particles and analyzed the cell proliferation and cell-scaffold interaction. FPP-treated surface with titania and silica particles had micro asperities at low frequency. On the other hand, the alumina treated surface had micro asperities at high frequency. L929 fibroblasts were seeded on these specimens and then the number of cells was counted after 72 hours of culturing. The FPP-treated surfaces showed good cell proliferation comparing to polished surface. This indicates that micro asperities formed on the surface encourage cell adhesion. Cell adhesion behavior was evaluated by a scanning electron microscope (SEM) and a fluorescence microscope. Dense filopodia were observed when cells cultured on the FPP-treated surface. This means that FPP treatment enhances cell adhesion and proliferation. The number of cells observed on the FPP-treated surface depended on the shape of asperities formed by FPP treatment; the highest cell counts were obtained on alumina treated surface. This is because cell migration was not inhibited by the shape of alumina treated surface asperities.",
keywords = "biomaterial, biomedical, metal",
author = "Yuta Kurashina and Shogo Miyata and Jun Komotori and Tadayoshi Koyama",
year = "2014",
month = "11",
day = "17",
doi = "10.1557/opl.2014.228",
language = "English",
volume = "1648",
journal = "Prehospital and Disaster Medicine",
issn = "1049-023X",
publisher = "World Association for Disaster and Emergency Medicine",
number = "4",

}

TY - JOUR

T1 - Proliferation and adhesion of L929 fibroblasts on surface with different microtopography

AU - Kurashina, Yuta

AU - Miyata, Shogo

AU - Komotori, Jun

AU - Koyama, Tadayoshi

PY - 2014/11/17

Y1 - 2014/11/17

N2 - Three types of 316L stainless steel surface with different topography were prepared by a Fine Particle Peening (FPP) treatment using titania, silica and alumina shot particles and analyzed the cell proliferation and cell-scaffold interaction. FPP-treated surface with titania and silica particles had micro asperities at low frequency. On the other hand, the alumina treated surface had micro asperities at high frequency. L929 fibroblasts were seeded on these specimens and then the number of cells was counted after 72 hours of culturing. The FPP-treated surfaces showed good cell proliferation comparing to polished surface. This indicates that micro asperities formed on the surface encourage cell adhesion. Cell adhesion behavior was evaluated by a scanning electron microscope (SEM) and a fluorescence microscope. Dense filopodia were observed when cells cultured on the FPP-treated surface. This means that FPP treatment enhances cell adhesion and proliferation. The number of cells observed on the FPP-treated surface depended on the shape of asperities formed by FPP treatment; the highest cell counts were obtained on alumina treated surface. This is because cell migration was not inhibited by the shape of alumina treated surface asperities.

AB - Three types of 316L stainless steel surface with different topography were prepared by a Fine Particle Peening (FPP) treatment using titania, silica and alumina shot particles and analyzed the cell proliferation and cell-scaffold interaction. FPP-treated surface with titania and silica particles had micro asperities at low frequency. On the other hand, the alumina treated surface had micro asperities at high frequency. L929 fibroblasts were seeded on these specimens and then the number of cells was counted after 72 hours of culturing. The FPP-treated surfaces showed good cell proliferation comparing to polished surface. This indicates that micro asperities formed on the surface encourage cell adhesion. Cell adhesion behavior was evaluated by a scanning electron microscope (SEM) and a fluorescence microscope. Dense filopodia were observed when cells cultured on the FPP-treated surface. This means that FPP treatment enhances cell adhesion and proliferation. The number of cells observed on the FPP-treated surface depended on the shape of asperities formed by FPP treatment; the highest cell counts were obtained on alumina treated surface. This is because cell migration was not inhibited by the shape of alumina treated surface asperities.

KW - biomaterial

KW - biomedical

KW - metal

UR - http://www.scopus.com/inward/record.url?scp=84910635926&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84910635926&partnerID=8YFLogxK

U2 - 10.1557/opl.2014.228

DO - 10.1557/opl.2014.228

M3 - Article

AN - SCOPUS:84910635926

VL - 1648

JO - Prehospital and Disaster Medicine

JF - Prehospital and Disaster Medicine

SN - 1049-023X

IS - 4

ER -