Proliferation, differentiation and morphogenesis of fetal rat glandular stomach transplanted under the kidney capsule of syngeneic hosts

Masao Ichinose, Shinko Tsukada, Yasunobu Fujimitsu, Masae Tatematsu, Yasuo Matsubara, Naohisa Yahagi, Masashi Oka, Takehisa Suzuki, Yasuhito Shimizu, Satoshi Yonezawa, Takashi Kageyama, Kazumasa Miki, Hiroshi Fukamachi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Undifferentiated glandular stomach tissue fragments from 16.5-day fetal rats were transplanted under the kidney capsule of syngeneic adult rats, and the proliferation, differentiation and morphogenesis of the transplanted tissues were investigated. Gastric epithelial cells began to invaginate 3-4 days after the transplantation and immature glands were formed after 1 week. During the period there was a gradual increase in the expression of pepsinogen and cathepsin E, markers of cytodifferentiation of the stomach epithelia, both at protein and mRNA levels. Cathepsin E was weakly expressed in undifferentiated gastric epithelial cells at 16.5 days of gestation, and a higher level of the expression was observed in differentiated epithelia of the transplants. In contrast, the pepsinogen-producing cells first appeared around days 3-4 after transplantation and gradually increased in number to about 30% of the epithelial cells and became localized at the bottom of the gland. During the period of the experiment up to 1 month the pepsinogen- producing cells were all positive for class III mucin and cathepsin E, indicating the immature character of these cells. In addition, no parietal cells were observed. When the tissue fragments were transplanted into adrenalectomized animals, the epithelial differentiation and morphogenesis was suppressed, but its proliferation was enhanced. The observed changes were reversed by hydrocortisone replacement. These results suggest that the development of the 16.5-day fetal stomach is regulated intrinsically to a certain extent by the genetic program of the cells involved and various gastric functions develop in the absence of luminal stimulation, stage- specific systemic hormonal change, neuronal regulation or other systemic influences and that glucocorticoids modulate the developmental program of the fetal stomach tissues.

Original languageEnglish
Pages (from-to)635-642
Number of pages8
JournalDevelopment Growth and Differentiation
Volume39
Issue number5
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Morphogenesis
Capsules
Stomach
Cathepsin E
Kidney
Pepsinogen A
Epithelial Cells
Epithelium
Transplantation
Mucins
Glucocorticoids
Hydrocortisone
Fetus
Transplants
Pregnancy
Messenger RNA
Proteins

Keywords

  • Acid proteases
  • Glucocorticoids growth and differentiation
  • Morphogenesis
  • Stomach

ASJC Scopus subject areas

  • Anatomy
  • Developmental Biology
  • Cell Biology

Cite this

Proliferation, differentiation and morphogenesis of fetal rat glandular stomach transplanted under the kidney capsule of syngeneic hosts. / Ichinose, Masao; Tsukada, Shinko; Fujimitsu, Yasunobu; Tatematsu, Masae; Matsubara, Yasuo; Yahagi, Naohisa; Oka, Masashi; Suzuki, Takehisa; Shimizu, Yasuhito; Yonezawa, Satoshi; Kageyama, Takashi; Miki, Kazumasa; Fukamachi, Hiroshi.

In: Development Growth and Differentiation, Vol. 39, No. 5, 1997, p. 635-642.

Research output: Contribution to journalArticle

Ichinose, M, Tsukada, S, Fujimitsu, Y, Tatematsu, M, Matsubara, Y, Yahagi, N, Oka, M, Suzuki, T, Shimizu, Y, Yonezawa, S, Kageyama, T, Miki, K & Fukamachi, H 1997, 'Proliferation, differentiation and morphogenesis of fetal rat glandular stomach transplanted under the kidney capsule of syngeneic hosts', Development Growth and Differentiation, vol. 39, no. 5, pp. 635-642.
Ichinose, Masao ; Tsukada, Shinko ; Fujimitsu, Yasunobu ; Tatematsu, Masae ; Matsubara, Yasuo ; Yahagi, Naohisa ; Oka, Masashi ; Suzuki, Takehisa ; Shimizu, Yasuhito ; Yonezawa, Satoshi ; Kageyama, Takashi ; Miki, Kazumasa ; Fukamachi, Hiroshi. / Proliferation, differentiation and morphogenesis of fetal rat glandular stomach transplanted under the kidney capsule of syngeneic hosts. In: Development Growth and Differentiation. 1997 ; Vol. 39, No. 5. pp. 635-642.
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abstract = "Undifferentiated glandular stomach tissue fragments from 16.5-day fetal rats were transplanted under the kidney capsule of syngeneic adult rats, and the proliferation, differentiation and morphogenesis of the transplanted tissues were investigated. Gastric epithelial cells began to invaginate 3-4 days after the transplantation and immature glands were formed after 1 week. During the period there was a gradual increase in the expression of pepsinogen and cathepsin E, markers of cytodifferentiation of the stomach epithelia, both at protein and mRNA levels. Cathepsin E was weakly expressed in undifferentiated gastric epithelial cells at 16.5 days of gestation, and a higher level of the expression was observed in differentiated epithelia of the transplants. In contrast, the pepsinogen-producing cells first appeared around days 3-4 after transplantation and gradually increased in number to about 30{\%} of the epithelial cells and became localized at the bottom of the gland. During the period of the experiment up to 1 month the pepsinogen- producing cells were all positive for class III mucin and cathepsin E, indicating the immature character of these cells. In addition, no parietal cells were observed. When the tissue fragments were transplanted into adrenalectomized animals, the epithelial differentiation and morphogenesis was suppressed, but its proliferation was enhanced. The observed changes were reversed by hydrocortisone replacement. These results suggest that the development of the 16.5-day fetal stomach is regulated intrinsically to a certain extent by the genetic program of the cells involved and various gastric functions develop in the absence of luminal stimulation, stage- specific systemic hormonal change, neuronal regulation or other systemic influences and that glucocorticoids modulate the developmental program of the fetal stomach tissues.",
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T1 - Proliferation, differentiation and morphogenesis of fetal rat glandular stomach transplanted under the kidney capsule of syngeneic hosts

AU - Ichinose, Masao

AU - Tsukada, Shinko

AU - Fujimitsu, Yasunobu

AU - Tatematsu, Masae

AU - Matsubara, Yasuo

AU - Yahagi, Naohisa

AU - Oka, Masashi

AU - Suzuki, Takehisa

AU - Shimizu, Yasuhito

AU - Yonezawa, Satoshi

AU - Kageyama, Takashi

AU - Miki, Kazumasa

AU - Fukamachi, Hiroshi

PY - 1997

Y1 - 1997

N2 - Undifferentiated glandular stomach tissue fragments from 16.5-day fetal rats were transplanted under the kidney capsule of syngeneic adult rats, and the proliferation, differentiation and morphogenesis of the transplanted tissues were investigated. Gastric epithelial cells began to invaginate 3-4 days after the transplantation and immature glands were formed after 1 week. During the period there was a gradual increase in the expression of pepsinogen and cathepsin E, markers of cytodifferentiation of the stomach epithelia, both at protein and mRNA levels. Cathepsin E was weakly expressed in undifferentiated gastric epithelial cells at 16.5 days of gestation, and a higher level of the expression was observed in differentiated epithelia of the transplants. In contrast, the pepsinogen-producing cells first appeared around days 3-4 after transplantation and gradually increased in number to about 30% of the epithelial cells and became localized at the bottom of the gland. During the period of the experiment up to 1 month the pepsinogen- producing cells were all positive for class III mucin and cathepsin E, indicating the immature character of these cells. In addition, no parietal cells were observed. When the tissue fragments were transplanted into adrenalectomized animals, the epithelial differentiation and morphogenesis was suppressed, but its proliferation was enhanced. The observed changes were reversed by hydrocortisone replacement. These results suggest that the development of the 16.5-day fetal stomach is regulated intrinsically to a certain extent by the genetic program of the cells involved and various gastric functions develop in the absence of luminal stimulation, stage- specific systemic hormonal change, neuronal regulation or other systemic influences and that glucocorticoids modulate the developmental program of the fetal stomach tissues.

AB - Undifferentiated glandular stomach tissue fragments from 16.5-day fetal rats were transplanted under the kidney capsule of syngeneic adult rats, and the proliferation, differentiation and morphogenesis of the transplanted tissues were investigated. Gastric epithelial cells began to invaginate 3-4 days after the transplantation and immature glands were formed after 1 week. During the period there was a gradual increase in the expression of pepsinogen and cathepsin E, markers of cytodifferentiation of the stomach epithelia, both at protein and mRNA levels. Cathepsin E was weakly expressed in undifferentiated gastric epithelial cells at 16.5 days of gestation, and a higher level of the expression was observed in differentiated epithelia of the transplants. In contrast, the pepsinogen-producing cells first appeared around days 3-4 after transplantation and gradually increased in number to about 30% of the epithelial cells and became localized at the bottom of the gland. During the period of the experiment up to 1 month the pepsinogen- producing cells were all positive for class III mucin and cathepsin E, indicating the immature character of these cells. In addition, no parietal cells were observed. When the tissue fragments were transplanted into adrenalectomized animals, the epithelial differentiation and morphogenesis was suppressed, but its proliferation was enhanced. The observed changes were reversed by hydrocortisone replacement. These results suggest that the development of the 16.5-day fetal stomach is regulated intrinsically to a certain extent by the genetic program of the cells involved and various gastric functions develop in the absence of luminal stimulation, stage- specific systemic hormonal change, neuronal regulation or other systemic influences and that glucocorticoids modulate the developmental program of the fetal stomach tissues.

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KW - Glucocorticoids growth and differentiation

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