TY - JOUR
T1 - Proliferation of neural and neuronal progenitors after global brain ischemia in young adult macaque monkeys
AU - Tonchev, Anton B.
AU - Yamashima, Tetsumori
AU - Zhao, Liang
AU - Okano, Hirotaka James
AU - Okano, Hideyuki
N1 - Funding Information:
Research was supported by grants-in-aid for Strategic Promotion System for Brain Science (SPSBS) from the Japanese Ministry of Education, Culture, Sports, Science and Technology to T.Y., and Japan Science and Technology Corporation (Core Research for Evolutional Science and Technology) to H.O. We thank Drs. Masaharu Ogawa and Takaki Miyata for the gift of anti-Nestin antibody and Dr. Masashi Mizuguchi for providing anti-doublecortin antibody. A.B.T. is also indebted to Drs. George Chaldakov and Masahiko Watanabe for stimulating discussions, and to Dr. Ralica Petrova and Mrs. Kiyoko Wada for technical assistance.
PY - 2003/6/1
Y1 - 2003/6/1
N2 - To investigate the effect of global cerebral ischemia on brain cell proliferation in young adult macaques, we infused 5-bromo-2′-deoxyuridine (BrdU), a DNA replication indicator, into monkeys subjected to ischemia or sham-operated. Subsequent quantification by BrdU immunohistochemistry revealed a significant postischemic increase in the number of BrdU-labeled cells in the hippocampal dentate gyrus, subventricular zone of the temporal horn of the lateral ventricle, and temporal neocortex. In all animals, 20-40% of the newly generated cells in the dentate gyrus and subventricular zone expressed the neural progenitor cell markers Musashi1 or Nestin. A few BrdU-positive cells in postischemic monkeys were double-stained for markers of neuronal progenitors (class III β-tubulin, TUC4, doublecortin, or Hu), neurons (NeuN), or glia (S100β or GFAP). Our results suggest that ischemia activates endogenous neuronal and glial precursors residing in diverse locations of the adult primate central nervous system.
AB - To investigate the effect of global cerebral ischemia on brain cell proliferation in young adult macaques, we infused 5-bromo-2′-deoxyuridine (BrdU), a DNA replication indicator, into monkeys subjected to ischemia or sham-operated. Subsequent quantification by BrdU immunohistochemistry revealed a significant postischemic increase in the number of BrdU-labeled cells in the hippocampal dentate gyrus, subventricular zone of the temporal horn of the lateral ventricle, and temporal neocortex. In all animals, 20-40% of the newly generated cells in the dentate gyrus and subventricular zone expressed the neural progenitor cell markers Musashi1 or Nestin. A few BrdU-positive cells in postischemic monkeys were double-stained for markers of neuronal progenitors (class III β-tubulin, TUC4, doublecortin, or Hu), neurons (NeuN), or glia (S100β or GFAP). Our results suggest that ischemia activates endogenous neuronal and glial precursors residing in diverse locations of the adult primate central nervous system.
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U2 - 10.1016/S1044-7431(03)00058-7
DO - 10.1016/S1044-7431(03)00058-7
M3 - Article
C2 - 12812760
AN - SCOPUS:0038721206
SN - 1044-7431
VL - 23
SP - 292
EP - 301
JO - Molecular and Cellular Neurosciences
JF - Molecular and Cellular Neurosciences
IS - 2
ER -