Proliferative behavior of the murine cerebral wall in tissue culture: Cell cycle kinetics and checkpoints

Takao Takahashi, P. G. Bhide, T. Goto, S. Miyama, V. S. Caviness

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Cerebral wall from embryonic day 13 mice was cultured in a three- dimensional collagen matrix in defined, serum-free medium. The cerebral wall retained its normal architecture, including the radial glial fiber system, for up to 19 h in culture. The cell cycle was initially blocked at the S/G2/M and the G1/S phase transitions, resulting in a transient synchronization of the proliferative cells. The transient blockades correspond, we suggest, to the G2 checkpoint and G1 restriction point, adaptive mechanisms of normal proliferative cells. The blocks were relieved within a few hours of explantation with restoration of the interkinetic nuclear migration and flow of cells through the cycle phases. The duration of the reestablished cell cycle and those of G1, S, and combined G2-M phases were estimated to be 19.2, 6.3-8.3, 8.8, and 2.0-4.0 h, respectively. The leaving (Q) fraction of the cycle (0.64) was twice the in vivo value. Two-thirds of the Q fraction cells remained in the ventricular epithelium, resulting in a substantially low growth fraction of 0.73 compared with 1.0 in vivo. The embryonic murine cerebral explant, cultured in minimum essential medium, should be favorable for studies of cycle modulatory actions of cell external influences such as growth factors or neurotransmitters.

Original languageEnglish
Pages (from-to)407-417
Number of pages11
JournalExperimental Neurology
Volume156
Issue number2
DOIs
Publication statusPublished - 1999 Apr

Fingerprint

Cell Cycle Checkpoints
Cell Cycle
G2 Phase Cell Cycle Checkpoints
G1 Phase Cell Cycle Checkpoints
G2 Phase
Phase Transition
Serum-Free Culture Media
G1 Phase
S Phase
Neuroglia
Cell Division
Neurotransmitter Agents
Intercellular Signaling Peptides and Proteins
Collagen
Epithelium
Growth

Keywords

  • Cell cycle
  • Mouse
  • Neocortical development
  • Organotypic explants
  • Tissue culture

ASJC Scopus subject areas

  • Neurology
  • Neuroscience(all)

Cite this

Proliferative behavior of the murine cerebral wall in tissue culture : Cell cycle kinetics and checkpoints. / Takahashi, Takao; Bhide, P. G.; Goto, T.; Miyama, S.; Caviness, V. S.

In: Experimental Neurology, Vol. 156, No. 2, 04.1999, p. 407-417.

Research output: Contribution to journalArticle

Takahashi, Takao ; Bhide, P. G. ; Goto, T. ; Miyama, S. ; Caviness, V. S. / Proliferative behavior of the murine cerebral wall in tissue culture : Cell cycle kinetics and checkpoints. In: Experimental Neurology. 1999 ; Vol. 156, No. 2. pp. 407-417.
@article{6147645fd903466bb9eb55c04b2d3885,
title = "Proliferative behavior of the murine cerebral wall in tissue culture: Cell cycle kinetics and checkpoints",
abstract = "Cerebral wall from embryonic day 13 mice was cultured in a three- dimensional collagen matrix in defined, serum-free medium. The cerebral wall retained its normal architecture, including the radial glial fiber system, for up to 19 h in culture. The cell cycle was initially blocked at the S/G2/M and the G1/S phase transitions, resulting in a transient synchronization of the proliferative cells. The transient blockades correspond, we suggest, to the G2 checkpoint and G1 restriction point, adaptive mechanisms of normal proliferative cells. The blocks were relieved within a few hours of explantation with restoration of the interkinetic nuclear migration and flow of cells through the cycle phases. The duration of the reestablished cell cycle and those of G1, S, and combined G2-M phases were estimated to be 19.2, 6.3-8.3, 8.8, and 2.0-4.0 h, respectively. The leaving (Q) fraction of the cycle (0.64) was twice the in vivo value. Two-thirds of the Q fraction cells remained in the ventricular epithelium, resulting in a substantially low growth fraction of 0.73 compared with 1.0 in vivo. The embryonic murine cerebral explant, cultured in minimum essential medium, should be favorable for studies of cycle modulatory actions of cell external influences such as growth factors or neurotransmitters.",
keywords = "Cell cycle, Mouse, Neocortical development, Organotypic explants, Tissue culture",
author = "Takao Takahashi and Bhide, {P. G.} and T. Goto and S. Miyama and Caviness, {V. S.}",
year = "1999",
month = "4",
doi = "10.1006/exnr.1999.7023",
language = "English",
volume = "156",
pages = "407--417",
journal = "Experimental Neurology",
issn = "0014-4886",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Proliferative behavior of the murine cerebral wall in tissue culture

T2 - Cell cycle kinetics and checkpoints

AU - Takahashi, Takao

AU - Bhide, P. G.

AU - Goto, T.

AU - Miyama, S.

AU - Caviness, V. S.

PY - 1999/4

Y1 - 1999/4

N2 - Cerebral wall from embryonic day 13 mice was cultured in a three- dimensional collagen matrix in defined, serum-free medium. The cerebral wall retained its normal architecture, including the radial glial fiber system, for up to 19 h in culture. The cell cycle was initially blocked at the S/G2/M and the G1/S phase transitions, resulting in a transient synchronization of the proliferative cells. The transient blockades correspond, we suggest, to the G2 checkpoint and G1 restriction point, adaptive mechanisms of normal proliferative cells. The blocks were relieved within a few hours of explantation with restoration of the interkinetic nuclear migration and flow of cells through the cycle phases. The duration of the reestablished cell cycle and those of G1, S, and combined G2-M phases were estimated to be 19.2, 6.3-8.3, 8.8, and 2.0-4.0 h, respectively. The leaving (Q) fraction of the cycle (0.64) was twice the in vivo value. Two-thirds of the Q fraction cells remained in the ventricular epithelium, resulting in a substantially low growth fraction of 0.73 compared with 1.0 in vivo. The embryonic murine cerebral explant, cultured in minimum essential medium, should be favorable for studies of cycle modulatory actions of cell external influences such as growth factors or neurotransmitters.

AB - Cerebral wall from embryonic day 13 mice was cultured in a three- dimensional collagen matrix in defined, serum-free medium. The cerebral wall retained its normal architecture, including the radial glial fiber system, for up to 19 h in culture. The cell cycle was initially blocked at the S/G2/M and the G1/S phase transitions, resulting in a transient synchronization of the proliferative cells. The transient blockades correspond, we suggest, to the G2 checkpoint and G1 restriction point, adaptive mechanisms of normal proliferative cells. The blocks were relieved within a few hours of explantation with restoration of the interkinetic nuclear migration and flow of cells through the cycle phases. The duration of the reestablished cell cycle and those of G1, S, and combined G2-M phases were estimated to be 19.2, 6.3-8.3, 8.8, and 2.0-4.0 h, respectively. The leaving (Q) fraction of the cycle (0.64) was twice the in vivo value. Two-thirds of the Q fraction cells remained in the ventricular epithelium, resulting in a substantially low growth fraction of 0.73 compared with 1.0 in vivo. The embryonic murine cerebral explant, cultured in minimum essential medium, should be favorable for studies of cycle modulatory actions of cell external influences such as growth factors or neurotransmitters.

KW - Cell cycle

KW - Mouse

KW - Neocortical development

KW - Organotypic explants

KW - Tissue culture

UR - http://www.scopus.com/inward/record.url?scp=0032934938&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032934938&partnerID=8YFLogxK

U2 - 10.1006/exnr.1999.7023

DO - 10.1006/exnr.1999.7023

M3 - Article

C2 - 10328945

AN - SCOPUS:0032934938

VL - 156

SP - 407

EP - 417

JO - Experimental Neurology

JF - Experimental Neurology

SN - 0014-4886

IS - 2

ER -