Proliferative effect of human granulocyte colony-stimulating factor on blast cells of acute promyelocytic leukemia

Kiyoshi Kitano, Toshio Suda, Yuko Sato, Yasusada Miura

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Abstract

The effect of human granulocyte colony-stimulating factor (G-CSF) on leukemic cells of acute promyelocytic leukemia (APL) was examined. Mononuclear cells obtained from bone marrow cells containing more than 90% blasts from seven APL patients were incubated in the presence of G-CSF using semisolid and liquid culture systems. On day 7, the cells from all the patients produced many clusters consisting of 8-40 cells. These cells appeared to be promyelocyte-like blast cells in four patients and had differentiated to more mature neutrophils in three patients. On day 14, the number of clusters decreased except for two patiens. Blast cells from the two patients showing the increase of blast clusters could proliferate in a liquid culture containing G-CSF. Blast cells cultured for 14 days formed many secondary cultures after replating on a methylcellulose medium. Moreover, chromosomal analyses of blasts cultivated in the presence of G-CSF for 7 days showed t(15;17) in all metaphases in one patient. It appears that the leukemic cells from APL patients could proliferate in the presence of G-CSF.

Original languageEnglish
Pages (from-to)843-849
Number of pages7
JournalJapanese Journal of Cancer Research
Volume79
Issue number7
Publication statusPublished - 1988 Jul

Fingerprint

Acute Promyelocytic Leukemia
Granulocyte Colony-Stimulating Factor
Granulocyte Precursor Cells
Methylcellulose
Metaphase
Bone Marrow Cells
Cultured Cells
Neutrophils

Keywords

  • Acute promyelocytic leukemia
  • Colony assay
  • Granulocyte colony-stimulating factor
  • Karyotype

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Proliferative effect of human granulocyte colony-stimulating factor on blast cells of acute promyelocytic leukemia. / Kitano, Kiyoshi; Suda, Toshio; Sato, Yuko; Miura, Yasusada.

In: Japanese Journal of Cancer Research, Vol. 79, No. 7, 07.1988, p. 843-849.

Research output: Contribution to journalArticle

Kitano, Kiyoshi ; Suda, Toshio ; Sato, Yuko ; Miura, Yasusada. / Proliferative effect of human granulocyte colony-stimulating factor on blast cells of acute promyelocytic leukemia. In: Japanese Journal of Cancer Research. 1988 ; Vol. 79, No. 7. pp. 843-849.
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