Prolonged survival of fully mismatched cardiac allografts and generation of regulatory cells by sairei-to, a japanese herbal medicine

Qi Zhang, Daiki Iwami, Osamu Aramaki, Shuji Yakubo, Ko Nishimura, Atsushi Ishige, Kenji Watanabe, Kenjiro Matsuno, Nozomu Shirasugi, Masanori Niimi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Sairei-to (TJ114), a 12-component Japanese herbal medicine, is used to treat immune-related diseases. We investigated the effects of oral administration of TJ114 in a murine model of cardiac transplantation with fully mismatched allografts. Untreated CBA mice rejected C57BL/6 hearts acutely (median survival time [MST], 7 days), whereas survival of allografts from mice given TJ114 was significantly prolonged (MST >100 days). Secondary CBA recipients of C57BL/6 hearts also had prolonged allograft survival (MST >100 days) after adoptive transfer of whole or CD4 splenocytes from primary CBA allograft recipients given TJ114. None of the individual components of TJ114 prolonged allograft survival, suggesting that its effects require administration of the combination agent. In mixed leukocyte cultures, proliferation of splenocytes from TJ114-treated CBA recipients was markedly suppressed compared with that of splenocytes from untreated mice, and interferon-γ production was significantly reduced. Thus, in our model, TJ114 treatment induced hyporesponsiveness to cardiac allografts and generated CD4 regulatory cells.

Original languageEnglish
Pages (from-to)1787-1791
Number of pages5
JournalTransplantation
Volume87
Issue number12
DOIs
Publication statusPublished - 2009 Jun 27

Fingerprint

Herbal Medicine
Allografts
Inbred CBA Mouse
Adoptive Transfer
sairei-to
Immune System Diseases
Heart Transplantation
Interferons
Oral Administration
Leukocytes

Keywords

  • Gorei-san
  • Kampo
  • Regulatory cells
  • Sairei-to
  • Sho-saiko-to

ASJC Scopus subject areas

  • Transplantation

Cite this

Prolonged survival of fully mismatched cardiac allografts and generation of regulatory cells by sairei-to, a japanese herbal medicine. / Zhang, Qi; Iwami, Daiki; Aramaki, Osamu; Yakubo, Shuji; Nishimura, Ko; Ishige, Atsushi; Watanabe, Kenji; Matsuno, Kenjiro; Shirasugi, Nozomu; Niimi, Masanori.

In: Transplantation, Vol. 87, No. 12, 27.06.2009, p. 1787-1791.

Research output: Contribution to journalArticle

Zhang, Q, Iwami, D, Aramaki, O, Yakubo, S, Nishimura, K, Ishige, A, Watanabe, K, Matsuno, K, Shirasugi, N & Niimi, M 2009, 'Prolonged survival of fully mismatched cardiac allografts and generation of regulatory cells by sairei-to, a japanese herbal medicine', Transplantation, vol. 87, no. 12, pp. 1787-1791. https://doi.org/10.1097/TP.0b013e3181a75917
Zhang, Qi ; Iwami, Daiki ; Aramaki, Osamu ; Yakubo, Shuji ; Nishimura, Ko ; Ishige, Atsushi ; Watanabe, Kenji ; Matsuno, Kenjiro ; Shirasugi, Nozomu ; Niimi, Masanori. / Prolonged survival of fully mismatched cardiac allografts and generation of regulatory cells by sairei-to, a japanese herbal medicine. In: Transplantation. 2009 ; Vol. 87, No. 12. pp. 1787-1791.
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