Promoted cell proliferation by connexin 30 gene transfection to head-and-neck cancer cell line

Hiroyuki Ozawa, Hideki Mutai, Tatsuo Matsunaga, Yutaka Tokumaru, Masato Fujii, Kouji Sakamoto, Toshiki Tomita, Kaoru Ogawa

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: There have been inconsistent results regarding the contribution of the connexin family of genes to tumor cell proliferation. Materials and Methods: We aimed to clarify the role of connexin 30 (Cx30), by transfecting three kinds of vectors that express either full length Cx30 (Cx30-Full), Cx30 devoid of C-terminal region (Cx30-DelC) or Cx30 C-terminal region (Cx30-CT), in HSC-4, a head-and-neck cancer cell line. Results: Transfected Cx30-Full was localized on the plasma membrane, while Cx30-DelC and Cx30-CT was expressed in the cytoplasm or circumnuclear sites. We studied the effect on the growth rate followed by immunostaining with anti-Ki-67 (MIB-1). The MIB indices of HSC-4 cells transfected with Cx30-Full and Cx30-DelC, but not Cx30-CT were shown to be significantly higher than that of the controls. Conclusion: Our results demonstrated that Cx30 enhanced the proliferation of HSC-4 cells and the proliferating activity was considered to be achieved without the transport of the protein onto the plasma membrane.

Original languageEnglish
Pages (from-to)1981-1985
Number of pages5
JournalAnticancer Research
Volume29
Issue number6
Publication statusPublished - 2009 Jun

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Connexins
Head and Neck Neoplasms
Transfection
Cell Proliferation
Cell Line
Genes
Cell Membrane

Keywords

  • Cell proliferation
  • Connexin
  • Gap junction protein
  • Head-and-neck cancer

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ozawa, H., Mutai, H., Matsunaga, T., Tokumaru, Y., Fujii, M., Sakamoto, K., ... Ogawa, K. (2009). Promoted cell proliferation by connexin 30 gene transfection to head-and-neck cancer cell line. Anticancer Research, 29(6), 1981-1985.

Promoted cell proliferation by connexin 30 gene transfection to head-and-neck cancer cell line. / Ozawa, Hiroyuki; Mutai, Hideki; Matsunaga, Tatsuo; Tokumaru, Yutaka; Fujii, Masato; Sakamoto, Kouji; Tomita, Toshiki; Ogawa, Kaoru.

In: Anticancer Research, Vol. 29, No. 6, 06.2009, p. 1981-1985.

Research output: Contribution to journalArticle

Ozawa, H, Mutai, H, Matsunaga, T, Tokumaru, Y, Fujii, M, Sakamoto, K, Tomita, T & Ogawa, K 2009, 'Promoted cell proliferation by connexin 30 gene transfection to head-and-neck cancer cell line', Anticancer Research, vol. 29, no. 6, pp. 1981-1985.
Ozawa H, Mutai H, Matsunaga T, Tokumaru Y, Fujii M, Sakamoto K et al. Promoted cell proliferation by connexin 30 gene transfection to head-and-neck cancer cell line. Anticancer Research. 2009 Jun;29(6):1981-1985.
Ozawa, Hiroyuki ; Mutai, Hideki ; Matsunaga, Tatsuo ; Tokumaru, Yutaka ; Fujii, Masato ; Sakamoto, Kouji ; Tomita, Toshiki ; Ogawa, Kaoru. / Promoted cell proliferation by connexin 30 gene transfection to head-and-neck cancer cell line. In: Anticancer Research. 2009 ; Vol. 29, No. 6. pp. 1981-1985.
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