Abstract
We examined the spatial expression pattern of medaka fish (Oryzias latipes) soluble guanylate cyclase α1 and β1 subunit genes, OlGCS-α1 and OIGCS-β1, and characterized the 5′-flanking region required for expression of both genes by introducing various promoter-luciferase fusion-gene constructs into COS-1 cells and medaka fish embryos. The OlGCS-α1 and OIGCS-β1 gene transcripts were detected in whole brain and kidney in 7-day and 9-day embryos. Primer-extension analysis demonstrated that there were no differences among various adult organs (brain, eye, kidney, ovary and testis) in the transcription start site of the OIGCS-α1 and OIGCS-β1 genes. Neither gene contained the functional TATA box within its 5′-flanking region, and the basal promoter activity was found between nucleotides +33 and +42 in the OIGCS-α1 gene and between nucleotides + 146 and + 155 in the OIGCS-β1 gene. In the assay of medaka fish embryos, the 5′-flanking region of the OIGCS-β1 gene exhibited lower promoter activity than that of the OIGCS-α1 gene. In the experiments on dual-luciferase fusion-gene constructs, the 5′-flanking region of the OIGCS-α1 gene connected to the 5′-flanking region of the OIGCS-β1 gene was introduced into medaka fish embryos, and the 5′-flanking regions of both subunit genes were shown to mutually influence each other's promoter activity.
| Original language | English |
|---|---|
| Pages (from-to) | 337-345 |
| Number of pages | 9 |
| Journal | Biochemical Journal |
| Volume | 361 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2002 Jan 15 |
| Externally published | Yes |
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Keywords
- cGMP
- Co-ordinated transcription
- Dual-luciferase assay
- Reporter fusion-gene construct
ASJC Scopus subject areas
- Biochemistry
Cite this
Promoter activity of the 5′-flanking regions of medaka fish soluble guanylate cyclase α1 and β subunit genes. / Yamamoto, Takehiro; Suzuki, Norio.
In: Biochemical Journal, Vol. 361, No. 2, 15.01.2002, p. 337-345.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Promoter activity of the 5′-flanking regions of medaka fish soluble guanylate cyclase α1 and β subunit genes
AU - Yamamoto, Takehiro
AU - Suzuki, Norio
PY - 2002/1/15
Y1 - 2002/1/15
N2 - We examined the spatial expression pattern of medaka fish (Oryzias latipes) soluble guanylate cyclase α1 and β1 subunit genes, OlGCS-α1 and OIGCS-β1, and characterized the 5′-flanking region required for expression of both genes by introducing various promoter-luciferase fusion-gene constructs into COS-1 cells and medaka fish embryos. The OlGCS-α1 and OIGCS-β1 gene transcripts were detected in whole brain and kidney in 7-day and 9-day embryos. Primer-extension analysis demonstrated that there were no differences among various adult organs (brain, eye, kidney, ovary and testis) in the transcription start site of the OIGCS-α1 and OIGCS-β1 genes. Neither gene contained the functional TATA box within its 5′-flanking region, and the basal promoter activity was found between nucleotides +33 and +42 in the OIGCS-α1 gene and between nucleotides + 146 and + 155 in the OIGCS-β1 gene. In the assay of medaka fish embryos, the 5′-flanking region of the OIGCS-β1 gene exhibited lower promoter activity than that of the OIGCS-α1 gene. In the experiments on dual-luciferase fusion-gene constructs, the 5′-flanking region of the OIGCS-α1 gene connected to the 5′-flanking region of the OIGCS-β1 gene was introduced into medaka fish embryos, and the 5′-flanking regions of both subunit genes were shown to mutually influence each other's promoter activity.
AB - We examined the spatial expression pattern of medaka fish (Oryzias latipes) soluble guanylate cyclase α1 and β1 subunit genes, OlGCS-α1 and OIGCS-β1, and characterized the 5′-flanking region required for expression of both genes by introducing various promoter-luciferase fusion-gene constructs into COS-1 cells and medaka fish embryos. The OlGCS-α1 and OIGCS-β1 gene transcripts were detected in whole brain and kidney in 7-day and 9-day embryos. Primer-extension analysis demonstrated that there were no differences among various adult organs (brain, eye, kidney, ovary and testis) in the transcription start site of the OIGCS-α1 and OIGCS-β1 genes. Neither gene contained the functional TATA box within its 5′-flanking region, and the basal promoter activity was found between nucleotides +33 and +42 in the OIGCS-α1 gene and between nucleotides + 146 and + 155 in the OIGCS-β1 gene. In the assay of medaka fish embryos, the 5′-flanking region of the OIGCS-β1 gene exhibited lower promoter activity than that of the OIGCS-α1 gene. In the experiments on dual-luciferase fusion-gene constructs, the 5′-flanking region of the OIGCS-α1 gene connected to the 5′-flanking region of the OIGCS-β1 gene was introduced into medaka fish embryos, and the 5′-flanking regions of both subunit genes were shown to mutually influence each other's promoter activity.
KW - cGMP
KW - Co-ordinated transcription
KW - Dual-luciferase assay
KW - Reporter fusion-gene construct
UR - http://www.scopus.com/inward/record.url?scp=0037081825&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037081825&partnerID=8YFLogxK
U2 - 10.1042/0264-6021:3610337
DO - 10.1042/0264-6021:3610337
M3 - Article
VL - 361
SP - 337
EP - 345
JO - Biochemical Journal
JF - Biochemical Journal
SN - 0264-6021
IS - 2
ER -