Promutagen activation by Vicia faba: An assay based on the induction of sister-chromatid exchanges in Chinese hamster ovary cells

Shin Takehisa, Nobuhiro Kanaya, Rigomar Rieger

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Abstract

Plant activation of promutagens was studied using Vicia faba S10 (in vitro activation) and the extracts prepared from promutagen-treated roots of Vicia faba (in vivo activation). The induction of sister-chromatid exchanges in Chinese hamster ovary cells was used as an endpoint to evaluate the cytogenetic effects of promutagens activated by Vicia faba. Cyclophosphamide and ethyl alcohol were activated both by Vicia S10 and by the Vicia extracts, and their activation resulted in an increase in SCEs. Benzo[a]pyrene, 2-aminofluorene, and maleic hydrazide were not activated. Aniline was activated, but without effect on the induction of SCEs. The activation capacity in vitro and in vivo of Vicia faba not very pronounced, except for the activation of ethyl alcohol, when compared with that of rat-liver S9, and showed differences in activation for the 6 chemical agents tested.

Original languageEnglish
Pages (from-to)195-205
Number of pages11
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume197
Issue number2
DOIs
Publication statusPublished - 1988 Feb

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Keywords

  • Chinese hamster ovary cells
  • Cyclophosphamide
  • Ethanol
  • Promutagen activation
  • SCE induction
  • Vicia faba

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

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