(Pro)renin receptor accelerates development of sarcopenia via activation of Wnt/YAP signaling axis

Naohiro Yoshida, Jin Endo, Kenichiro Kinouchi, Hiroki Kitakata, Hidenori Moriyama, Masaharu Kataoka, Tsunehisa Yamamoto, Kohsuke Shirakawa, Satoshi Morimoto, Akira Nishiyama, Akihiro Hashiguchi, Itsuro Higuchi, Keiichi Fukuda, Atsuhiro Ichihara, Motoaki Sano

Research output: Contribution to journalArticle

Abstract

To extend life expectancy and ensure healthy aging, it is crucial to prevent and minimize age-induced skeletal muscle atrophy, also known as sarcopenia. However, the disease's molecular mechanism remains unclear. The age-related Wnt/β-catenin signaling pathway has been recently shown to be activated by the (pro)renin receptor ((P)RR). We report here that (P)RR expression was increased in the atrophied skeletal muscles of aged mice and humans. Therefore, we developed a gain-of-function model of age-related sarcopenia via transgenic expression of (P)RR under control of the CAG promoter. Consistent with our hypothesis, (P)RR-Tg mice died early and exhibited muscle atrophy with histological features of sarcopenia. Moreover, Wnt/β-catenin signaling was activated and the regenerative capacity of muscle progenitor cells after cardiotoxin injury was impaired due to cell fusion failure in (P)RR-Tg mice. In vitro forced expression of (P)RR protein in C2C12 myoblast cells suppressed myotube formation by activating Wnt/β-catenin signaling. Administration of Dickkopf-related protein 1, an inhibitor of Wnt/β-catenin signaling, and anti-(P)RR neutralizing antibody, which inhibits binding of (P)RR to the Wnt receptor, significantly improved sarcopenia in (P)RR-Tg mice. Furthermore, the use of anti-(P)RR neutralizing antibodies significantly improved the regenerative ability of skeletal muscle in aged mice. Finally, we show that Yes-associated protein (YAP) signaling, which is coordinately regulated by Wnt/β-catenin, contributed to the development of (P)RR-induced sarcopenia. The present study demonstrates the use of (P)RR-Tg mice as a novel sarcopenia model, and shows that (P)RR-Wnt-YAP signaling plays a pivotal role in the pathogenesis of this disease.

Original languageEnglish
Article numbere12991
JournalAging Cell
Volume18
Issue number5
DOIs
Publication statusPublished - 2019 Oct 1

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Sarcopenia
Catenins
Renin
Skeletal Muscle
Proteins
Muscular Atrophy
Neutralizing Antibodies
Wnt1 Protein
Wnt Receptors
Cardiotoxins
Wnt Signaling Pathway
Cell Fusion
Myoblasts
Skeletal Muscle Fibers
Life Expectancy
Muscle Cells
Stem Cells
Wounds and Injuries

Keywords

  • (pro)renin receptor
  • aging
  • canonical Wnt pathway
  • sarcopenia
  • skeletal muscle atrophy
  • YAP signaling

ASJC Scopus subject areas

  • Ageing
  • Cell Biology

Cite this

(Pro)renin receptor accelerates development of sarcopenia via activation of Wnt/YAP signaling axis. / Yoshida, Naohiro; Endo, Jin; Kinouchi, Kenichiro; Kitakata, Hiroki; Moriyama, Hidenori; Kataoka, Masaharu; Yamamoto, Tsunehisa; Shirakawa, Kohsuke; Morimoto, Satoshi; Nishiyama, Akira; Hashiguchi, Akihiro; Higuchi, Itsuro; Fukuda, Keiichi; Ichihara, Atsuhiro; Sano, Motoaki.

In: Aging Cell, Vol. 18, No. 5, e12991, 01.10.2019.

Research output: Contribution to journalArticle

Yoshida, N, Endo, J, Kinouchi, K, Kitakata, H, Moriyama, H, Kataoka, M, Yamamoto, T, Shirakawa, K, Morimoto, S, Nishiyama, A, Hashiguchi, A, Higuchi, I, Fukuda, K, Ichihara, A & Sano, M 2019, '(Pro)renin receptor accelerates development of sarcopenia via activation of Wnt/YAP signaling axis', Aging Cell, vol. 18, no. 5, e12991. https://doi.org/10.1111/acel.12991
Yoshida, Naohiro ; Endo, Jin ; Kinouchi, Kenichiro ; Kitakata, Hiroki ; Moriyama, Hidenori ; Kataoka, Masaharu ; Yamamoto, Tsunehisa ; Shirakawa, Kohsuke ; Morimoto, Satoshi ; Nishiyama, Akira ; Hashiguchi, Akihiro ; Higuchi, Itsuro ; Fukuda, Keiichi ; Ichihara, Atsuhiro ; Sano, Motoaki. / (Pro)renin receptor accelerates development of sarcopenia via activation of Wnt/YAP signaling axis. In: Aging Cell. 2019 ; Vol. 18, No. 5.
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AU - Moriyama, Hidenori

AU - Kataoka, Masaharu

AU - Yamamoto, Tsunehisa

AU - Shirakawa, Kohsuke

AU - Morimoto, Satoshi

AU - Nishiyama, Akira

AU - Hashiguchi, Akihiro

AU - Higuchi, Itsuro

AU - Fukuda, Keiichi

AU - Ichihara, Atsuhiro

AU - Sano, Motoaki

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