Prorenin receptor is essential for normal podocyte structure and function

Yoichi Oshima, Kenichiro Kinouchi, Atsuhiro Ichihara, Mariyo Sakoda, Asako Kurauchi-Mito, Kanako Bokuda, Tatsuya Narita, Hideaki Kurosawa, Ge Hong Sun-Wada, Yoh Wada, Taketo Yamada, Minoru Takemoto, Moin A. Saleem, Susan E. Quaggin, Hiroshi Itoh

Research output: Contribution to journalArticlepeer-review

123 Citations (Scopus)

Abstract

The prorenin receptor is an accessory subunit of the vacuolar H +-ATPase, suggesting that it has fundamental functions beyond activation of the local renin-angiotensin system. Podocytes express the prorenin receptor, but its function in these cells is unknown. Here, podocyte-specific, conditional, prorenin receptor-knockout mice died of kidney failure and severe proteinuria within 4 weeks of birth. The podocytes of these mice exhibited foot process effacement with reduced and altered localization of the slit-diaphragm proteins nephrin and podocin. Furthermore, the podocytes contained numerous autophagic vacuoles, confirmed by enhanced accumulation of microtubule- associated protein 1 light chain 3-positive intracellular vesicles. Ablation of the prorenin receptor selectively suppressed expression of the V 0 c-subunit of the vacuolar H +-ATPase in podocytes, resulting in deacidification of intracellular vesicles. In conclusion, the prorenin receptor is important for the maintenance of normal podocyte structure and function.

Original languageEnglish
Pages (from-to)2203-2212
Number of pages10
JournalJournal of the American Society of Nephrology
Volume22
Issue number12
DOIs
Publication statusPublished - 2011 Dec

ASJC Scopus subject areas

  • Nephrology

Fingerprint Dive into the research topics of 'Prorenin receptor is essential for normal podocyte structure and function'. Together they form a unique fingerprint.

Cite this