Prorenin receptor is essential for normal podocyte structure and function

Yoichi Oshima, Kenichiro Kinouchi, Atsuhiro Ichihara, Mariyo Sakoda, Asako Kurauchi-Mito, Kanako Bokuda, Tatsuya Narita, Hideaki Kurosawa, Ge Hong Sun-Wada, Yoh Wada, Taketo Yamada, Minoru Takemoto, Moin A. Saleem, Susan E. Quaggin, Hiroshi Itoh

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

The prorenin receptor is an accessory subunit of the vacuolar H +-ATPase, suggesting that it has fundamental functions beyond activation of the local renin-angiotensin system. Podocytes express the prorenin receptor, but its function in these cells is unknown. Here, podocyte-specific, conditional, prorenin receptor-knockout mice died of kidney failure and severe proteinuria within 4 weeks of birth. The podocytes of these mice exhibited foot process effacement with reduced and altered localization of the slit-diaphragm proteins nephrin and podocin. Furthermore, the podocytes contained numerous autophagic vacuoles, confirmed by enhanced accumulation of microtubule- associated protein 1 light chain 3-positive intracellular vesicles. Ablation of the prorenin receptor selectively suppressed expression of the V 0 c-subunit of the vacuolar H +-ATPase in podocytes, resulting in deacidification of intracellular vesicles. In conclusion, the prorenin receptor is important for the maintenance of normal podocyte structure and function.

Original languageEnglish
Pages (from-to)2203-2212
Number of pages10
JournalJournal of the American Society of Nephrology
Volume22
Issue number12
DOIs
Publication statusPublished - 2011 Dec

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Podocytes
Vacuolar Proton-Translocating ATPases
Microtubule-Associated Proteins
Renin-Angiotensin System
Vacuoles
Diaphragm
Proteinuria
Knockout Mice
Renal Insufficiency
prorenin receptor
Foot
Maintenance
Parturition
Light
Proteins

ASJC Scopus subject areas

  • Nephrology

Cite this

Prorenin receptor is essential for normal podocyte structure and function. / Oshima, Yoichi; Kinouchi, Kenichiro; Ichihara, Atsuhiro; Sakoda, Mariyo; Kurauchi-Mito, Asako; Bokuda, Kanako; Narita, Tatsuya; Kurosawa, Hideaki; Sun-Wada, Ge Hong; Wada, Yoh; Yamada, Taketo; Takemoto, Minoru; Saleem, Moin A.; Quaggin, Susan E.; Itoh, Hiroshi.

In: Journal of the American Society of Nephrology, Vol. 22, No. 12, 12.2011, p. 2203-2212.

Research output: Contribution to journalArticle

Oshima, Y, Kinouchi, K, Ichihara, A, Sakoda, M, Kurauchi-Mito, A, Bokuda, K, Narita, T, Kurosawa, H, Sun-Wada, GH, Wada, Y, Yamada, T, Takemoto, M, Saleem, MA, Quaggin, SE & Itoh, H 2011, 'Prorenin receptor is essential for normal podocyte structure and function', Journal of the American Society of Nephrology, vol. 22, no. 12, pp. 2203-2212. https://doi.org/10.1681/ASN.2011020202
Oshima Y, Kinouchi K, Ichihara A, Sakoda M, Kurauchi-Mito A, Bokuda K et al. Prorenin receptor is essential for normal podocyte structure and function. Journal of the American Society of Nephrology. 2011 Dec;22(12):2203-2212. https://doi.org/10.1681/ASN.2011020202
Oshima, Yoichi ; Kinouchi, Kenichiro ; Ichihara, Atsuhiro ; Sakoda, Mariyo ; Kurauchi-Mito, Asako ; Bokuda, Kanako ; Narita, Tatsuya ; Kurosawa, Hideaki ; Sun-Wada, Ge Hong ; Wada, Yoh ; Yamada, Taketo ; Takemoto, Minoru ; Saleem, Moin A. ; Quaggin, Susan E. ; Itoh, Hiroshi. / Prorenin receptor is essential for normal podocyte structure and function. In: Journal of the American Society of Nephrology. 2011 ; Vol. 22, No. 12. pp. 2203-2212.
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