Prospective isolation of murine and human bone marrow mesenchymal stem cells based on surface markers

Yo Mabuchi, Diarmaid D. Houlihan, Chihiro Akazawa, Hideyuki Okano, Yumi Matsuzaki

Research output: Contribution to journalReview article

51 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSCs) are currently defined as multipotent stromal cells that undergo sustained in vitro growth and can give rise to cells of multiple mesenchymal lineages, such as adipocytes, chondrocytes, and osteoblasts. The regenerative and immunosuppressive properties of MSCs have led to numerous clinical trials exploring their utility for the treatment of a variety of diseases (e.g., acute graft-versus-host disease, Crohn's disease, multiple sclerosis, osteoarthritis, and cardiovascular diseases including heart failure and myocardial infarction). On the other hand, conventionally cultured MSCs reflect heterogeneous populations that often contain contaminating cells due to the significant variability in isolation methods and the lack of specific MSC markers. This review article focuses on recent developments in the MSC research field, with a special emphasis on the identification of novel surface markers for the in vivo localization and prospective isolation of murine and human MSCs. Furthermore, we discuss the physiological importance of MSC subtypes in vivo with specific reference to data supporting their contribution to HSC niche homeostasis. The isolation of MSCs using selective markers (combination of PDGFRα and Sca-1) is crucial to address the many unanswered questions pertaining to these cells and has the potential to enhance their therapeutic potential enormously.

Original languageEnglish
Article number507301
JournalStem Cells International
DOIs
Publication statusPublished - 2013 Jun 12

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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