Prostaglandin E2 increases both osteoblastic and osteoclastic activity in the scales and participates in calcium metabolism in goldfish

Katsunori Omori, Shigehito Wada, Yusuke Maruyama, Atsuhiko Hattori, Kei Ichiro Kitamura, Yusuke Sato, Masayuki Nara, Hisayuki Funahashi, Koji Yachiguchi, Kazuichi Hayakawa, Masato Endo, Rei Kusakari, Sachiko Yano, Ajai K. Srivastav, Takashi Kusui, Sadakazu Ejiri, Wenxi Chen, Yoshiaki Tabuchi, Yukihiro Furusawa, Takashi Kondo & 5 others Yuichi Sasayama, Takumi Nishiuchi, Masaki Nakano, Tatsuya Sakamoto, Nobuo Suzuki

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Using our original in vitro assay system with goldfish scales, we examined the direct effect of prostaglandin E2 (PGE2) on osteoclasts and osteoblasts in teleosts. In this assay system, we measured the activity of alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) as respective indicators of each activity in osteoblasts and osteoclasts. ALP activity in scales significantly increased following treatment at high concentration of PGE2 (10-7 and 10-6 M) over 6 hrs of incubation. At 18 hrs of incubation, ALP activity also significantly increased in the PGE2 (10-9 to 10-6 M)-treated scale. In the case of osteoclasts, TRAP activity tended to increase at 6 hrs of incubation, and then significantly increased at 18 hrs of incubation by PGE 2(10-7 to 10-6 M) treatment. At 18 hrs of incubation, the mRNA expression of osteoclastic markers (TRAP and cathepsin K) and receptor activator of the NF-B ligand (RANKL), an activating factor of osteoclasts expressed in osteoblasts, increased in PGE2 treated-scales. Thus, PGE2 acts on osteoblasts, and then increases the osteoclastic activity in the scales of goldfish as it does in the bone of mammals. In an in vivo experiment, plasma calcium levels and scale TRAP and ALP activities in the PGE2-injencted goldfish increased significantly. We conclude that, in teleosts, PGE2 activates both osteoblasts and osteoclasts and participates in calcium metabolism.

Original languageEnglish
Pages (from-to)499-504
Number of pages6
JournalZoological Science
Volume29
Issue number8
DOIs
Publication statusPublished - 2012 Aug

Fingerprint

goldfish
prostaglandins
osteoclasts
osteoblasts
calcium
metabolism
acid phosphatase
alkaline phosphatase
cathepsin K
assays
bones
mammals
receptors

Keywords

  • goldfish scales
  • osteoblasts
  • osteoclasts
  • plasma Ca
  • prostaglandin E
  • RANKL

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Prostaglandin E2 increases both osteoblastic and osteoclastic activity in the scales and participates in calcium metabolism in goldfish. / Omori, Katsunori; Wada, Shigehito; Maruyama, Yusuke; Hattori, Atsuhiko; Kitamura, Kei Ichiro; Sato, Yusuke; Nara, Masayuki; Funahashi, Hisayuki; Yachiguchi, Koji; Hayakawa, Kazuichi; Endo, Masato; Kusakari, Rei; Yano, Sachiko; Srivastav, Ajai K.; Kusui, Takashi; Ejiri, Sadakazu; Chen, Wenxi; Tabuchi, Yoshiaki; Furusawa, Yukihiro; Kondo, Takashi; Sasayama, Yuichi; Nishiuchi, Takumi; Nakano, Masaki; Sakamoto, Tatsuya; Suzuki, Nobuo.

In: Zoological Science, Vol. 29, No. 8, 08.2012, p. 499-504.

Research output: Contribution to journalArticle

Omori, K, Wada, S, Maruyama, Y, Hattori, A, Kitamura, KI, Sato, Y, Nara, M, Funahashi, H, Yachiguchi, K, Hayakawa, K, Endo, M, Kusakari, R, Yano, S, Srivastav, AK, Kusui, T, Ejiri, S, Chen, W, Tabuchi, Y, Furusawa, Y, Kondo, T, Sasayama, Y, Nishiuchi, T, Nakano, M, Sakamoto, T & Suzuki, N 2012, 'Prostaglandin E2 increases both osteoblastic and osteoclastic activity in the scales and participates in calcium metabolism in goldfish', Zoological Science, vol. 29, no. 8, pp. 499-504. https://doi.org/10.2108/zsj.29.499
Omori, Katsunori ; Wada, Shigehito ; Maruyama, Yusuke ; Hattori, Atsuhiko ; Kitamura, Kei Ichiro ; Sato, Yusuke ; Nara, Masayuki ; Funahashi, Hisayuki ; Yachiguchi, Koji ; Hayakawa, Kazuichi ; Endo, Masato ; Kusakari, Rei ; Yano, Sachiko ; Srivastav, Ajai K. ; Kusui, Takashi ; Ejiri, Sadakazu ; Chen, Wenxi ; Tabuchi, Yoshiaki ; Furusawa, Yukihiro ; Kondo, Takashi ; Sasayama, Yuichi ; Nishiuchi, Takumi ; Nakano, Masaki ; Sakamoto, Tatsuya ; Suzuki, Nobuo. / Prostaglandin E2 increases both osteoblastic and osteoclastic activity in the scales and participates in calcium metabolism in goldfish. In: Zoological Science. 2012 ; Vol. 29, No. 8. pp. 499-504.
@article{74d7b13ec8334df09f4715b58ebd8f08,
title = "Prostaglandin E2 increases both osteoblastic and osteoclastic activity in the scales and participates in calcium metabolism in goldfish",
abstract = "Using our original in vitro assay system with goldfish scales, we examined the direct effect of prostaglandin E2 (PGE2) on osteoclasts and osteoblasts in teleosts. In this assay system, we measured the activity of alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) as respective indicators of each activity in osteoblasts and osteoclasts. ALP activity in scales significantly increased following treatment at high concentration of PGE2 (10-7 and 10-6 M) over 6 hrs of incubation. At 18 hrs of incubation, ALP activity also significantly increased in the PGE2 (10-9 to 10-6 M)-treated scale. In the case of osteoclasts, TRAP activity tended to increase at 6 hrs of incubation, and then significantly increased at 18 hrs of incubation by PGE 2(10-7 to 10-6 M) treatment. At 18 hrs of incubation, the mRNA expression of osteoclastic markers (TRAP and cathepsin K) and receptor activator of the NF-B ligand (RANKL), an activating factor of osteoclasts expressed in osteoblasts, increased in PGE2 treated-scales. Thus, PGE2 acts on osteoblasts, and then increases the osteoclastic activity in the scales of goldfish as it does in the bone of mammals. In an in vivo experiment, plasma calcium levels and scale TRAP and ALP activities in the PGE2-injencted goldfish increased significantly. We conclude that, in teleosts, PGE2 activates both osteoblasts and osteoclasts and participates in calcium metabolism.",
keywords = "goldfish scales, osteoblasts, osteoclasts, plasma Ca, prostaglandin E, RANKL",
author = "Katsunori Omori and Shigehito Wada and Yusuke Maruyama and Atsuhiko Hattori and Kitamura, {Kei Ichiro} and Yusuke Sato and Masayuki Nara and Hisayuki Funahashi and Koji Yachiguchi and Kazuichi Hayakawa and Masato Endo and Rei Kusakari and Sachiko Yano and Srivastav, {Ajai K.} and Takashi Kusui and Sadakazu Ejiri and Wenxi Chen and Yoshiaki Tabuchi and Yukihiro Furusawa and Takashi Kondo and Yuichi Sasayama and Takumi Nishiuchi and Masaki Nakano and Tatsuya Sakamoto and Nobuo Suzuki",
year = "2012",
month = "8",
doi = "10.2108/zsj.29.499",
language = "English",
volume = "29",
pages = "499--504",
journal = "Zoological Science",
issn = "0289-0003",
publisher = "Zoological Society of Japan",
number = "8",

}

TY - JOUR

T1 - Prostaglandin E2 increases both osteoblastic and osteoclastic activity in the scales and participates in calcium metabolism in goldfish

AU - Omori, Katsunori

AU - Wada, Shigehito

AU - Maruyama, Yusuke

AU - Hattori, Atsuhiko

AU - Kitamura, Kei Ichiro

AU - Sato, Yusuke

AU - Nara, Masayuki

AU - Funahashi, Hisayuki

AU - Yachiguchi, Koji

AU - Hayakawa, Kazuichi

AU - Endo, Masato

AU - Kusakari, Rei

AU - Yano, Sachiko

AU - Srivastav, Ajai K.

AU - Kusui, Takashi

AU - Ejiri, Sadakazu

AU - Chen, Wenxi

AU - Tabuchi, Yoshiaki

AU - Furusawa, Yukihiro

AU - Kondo, Takashi

AU - Sasayama, Yuichi

AU - Nishiuchi, Takumi

AU - Nakano, Masaki

AU - Sakamoto, Tatsuya

AU - Suzuki, Nobuo

PY - 2012/8

Y1 - 2012/8

N2 - Using our original in vitro assay system with goldfish scales, we examined the direct effect of prostaglandin E2 (PGE2) on osteoclasts and osteoblasts in teleosts. In this assay system, we measured the activity of alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) as respective indicators of each activity in osteoblasts and osteoclasts. ALP activity in scales significantly increased following treatment at high concentration of PGE2 (10-7 and 10-6 M) over 6 hrs of incubation. At 18 hrs of incubation, ALP activity also significantly increased in the PGE2 (10-9 to 10-6 M)-treated scale. In the case of osteoclasts, TRAP activity tended to increase at 6 hrs of incubation, and then significantly increased at 18 hrs of incubation by PGE 2(10-7 to 10-6 M) treatment. At 18 hrs of incubation, the mRNA expression of osteoclastic markers (TRAP and cathepsin K) and receptor activator of the NF-B ligand (RANKL), an activating factor of osteoclasts expressed in osteoblasts, increased in PGE2 treated-scales. Thus, PGE2 acts on osteoblasts, and then increases the osteoclastic activity in the scales of goldfish as it does in the bone of mammals. In an in vivo experiment, plasma calcium levels and scale TRAP and ALP activities in the PGE2-injencted goldfish increased significantly. We conclude that, in teleosts, PGE2 activates both osteoblasts and osteoclasts and participates in calcium metabolism.

AB - Using our original in vitro assay system with goldfish scales, we examined the direct effect of prostaglandin E2 (PGE2) on osteoclasts and osteoblasts in teleosts. In this assay system, we measured the activity of alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) as respective indicators of each activity in osteoblasts and osteoclasts. ALP activity in scales significantly increased following treatment at high concentration of PGE2 (10-7 and 10-6 M) over 6 hrs of incubation. At 18 hrs of incubation, ALP activity also significantly increased in the PGE2 (10-9 to 10-6 M)-treated scale. In the case of osteoclasts, TRAP activity tended to increase at 6 hrs of incubation, and then significantly increased at 18 hrs of incubation by PGE 2(10-7 to 10-6 M) treatment. At 18 hrs of incubation, the mRNA expression of osteoclastic markers (TRAP and cathepsin K) and receptor activator of the NF-B ligand (RANKL), an activating factor of osteoclasts expressed in osteoblasts, increased in PGE2 treated-scales. Thus, PGE2 acts on osteoblasts, and then increases the osteoclastic activity in the scales of goldfish as it does in the bone of mammals. In an in vivo experiment, plasma calcium levels and scale TRAP and ALP activities in the PGE2-injencted goldfish increased significantly. We conclude that, in teleosts, PGE2 activates both osteoblasts and osteoclasts and participates in calcium metabolism.

KW - goldfish scales

KW - osteoblasts

KW - osteoclasts

KW - plasma Ca

KW - prostaglandin E

KW - RANKL

UR - http://www.scopus.com/inward/record.url?scp=84864955336&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864955336&partnerID=8YFLogxK

U2 - 10.2108/zsj.29.499

DO - 10.2108/zsj.29.499

M3 - Article

VL - 29

SP - 499

EP - 504

JO - Zoological Science

JF - Zoological Science

SN - 0289-0003

IS - 8

ER -