Prostaglandin E2 increases both osteoblastic and osteoclastic activity in the scales and participates in calcium metabolism in goldfish

Katsunori Omori, Shigehito Wada, Yusuke Maruyama, Atsuhiko Hattori, Kei Ichiro Kitamura, Yusuke Sato, Masayuki Nara, Hisayuki Funahashi, Koji Yachiguchi, Kazuichi Hayakawa, Masato Endo, Rei Kusakari, Sachiko Yano, Ajai K. Srivastav, Takashi Kusui, Sadakazu Ejiri, Wenxi Chen, Yoshiaki Tabuchi, Yukihiro Furusawa, Takashi KondoYuichi Sasayama, Takumi Nishiuchi, Masaki Nakano, Tatsuya Sakamoto, Nobuo Suzuki

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Using our original in vitro assay system with goldfish scales, we examined the direct effect of prostaglandin E2 (PGE2) on osteoclasts and osteoblasts in teleosts. In this assay system, we measured the activity of alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) as respective indicators of each activity in osteoblasts and osteoclasts. ALP activity in scales significantly increased following treatment at high concentration of PGE2 (10-7 and 10-6 M) over 6 hrs of incubation. At 18 hrs of incubation, ALP activity also significantly increased in the PGE2 (10-9 to 10-6 M)-treated scale. In the case of osteoclasts, TRAP activity tended to increase at 6 hrs of incubation, and then significantly increased at 18 hrs of incubation by PGE 2(10-7 to 10-6 M) treatment. At 18 hrs of incubation, the mRNA expression of osteoclastic markers (TRAP and cathepsin K) and receptor activator of the NF-B ligand (RANKL), an activating factor of osteoclasts expressed in osteoblasts, increased in PGE2 treated-scales. Thus, PGE2 acts on osteoblasts, and then increases the osteoclastic activity in the scales of goldfish as it does in the bone of mammals. In an in vivo experiment, plasma calcium levels and scale TRAP and ALP activities in the PGE2-injencted goldfish increased significantly. We conclude that, in teleosts, PGE2 activates both osteoblasts and osteoclasts and participates in calcium metabolism.

Original languageEnglish
Pages (from-to)499-504
Number of pages6
JournalZoological Science
Volume29
Issue number8
DOIs
Publication statusPublished - 2012 Aug

Keywords

  • RANKL
  • goldfish scales
  • osteoblasts
  • osteoclasts
  • plasma Ca
  • prostaglandin E

ASJC Scopus subject areas

  • Animal Science and Zoology

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