Prostaglandins and progesterone secretion in the in vitro perfused pseudopregnant rabbit ovary

A. M. Dharmarajan, Kou Sueoka, T. Miyazaki, S. J. Atlas, R. B. Ghodgaonkar, N. H. Dubin, B. R. Zirkin, E. E. Wallach

Research output: Contribution to journalArticle

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Abstract

The relationship between progesterone and prostaglandin (PG) secretion in the pseudopregnant rabbit corpus luteum was investigated using isolated in vitro perfused ovaries. Progesterone and PG secretion were measured on days 1, 11, and 18 of hCG-induced pseudopregnancy. The mean progesterone secretion increased significantly from days 1 to 11, and then decreased significantly by day 18. PG secretion was inversely correlated with progesterone secretion, suggesting that PG might inhibit progesterone secretion. To test this hypothesis, indomethacin, an inhibitor of PG secretion, was administered to intact rabbits from days 11-18 of pseudopregnancy and/or on day 18 ovaries were perfused in vitro with indomethacin. Indomethacin administered in vivo, in vitro, or both in vivo and in vitro significantly reduced PG secretion compared to that in controls, but did not affect progesterone secretion. In addition, perfusion of ovaries in vitro with PGF(2α) did not alter progesterone secretion on either day 11 or day 18. Thus, although there is an inverse relationship between progesterone and PG secretion during pseudopregnancy, PGF(2α) alone had no effect on progesterone secretion. These results question the hypothesis that PGF(2α) alone is the luteolytic factor.

Original languageEnglish
Pages (from-to)1198-1203
Number of pages6
JournalEndocrinology
Volume124
Issue number3
Publication statusPublished - 1989
Externally publishedYes

Fingerprint

Prostaglandins
Progesterone
Ovary
Rabbits
Pseudopregnancy
Prostaglandins F
Indomethacin
In Vitro Techniques
Prostaglandin Antagonists
Corpus Luteum
Perfusion

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Dharmarajan, A. M., Sueoka, K., Miyazaki, T., Atlas, S. J., Ghodgaonkar, R. B., Dubin, N. H., ... Wallach, E. E. (1989). Prostaglandins and progesterone secretion in the in vitro perfused pseudopregnant rabbit ovary. Endocrinology, 124(3), 1198-1203.

Prostaglandins and progesterone secretion in the in vitro perfused pseudopregnant rabbit ovary. / Dharmarajan, A. M.; Sueoka, Kou; Miyazaki, T.; Atlas, S. J.; Ghodgaonkar, R. B.; Dubin, N. H.; Zirkin, B. R.; Wallach, E. E.

In: Endocrinology, Vol. 124, No. 3, 1989, p. 1198-1203.

Research output: Contribution to journalArticle

Dharmarajan, AM, Sueoka, K, Miyazaki, T, Atlas, SJ, Ghodgaonkar, RB, Dubin, NH, Zirkin, BR & Wallach, EE 1989, 'Prostaglandins and progesterone secretion in the in vitro perfused pseudopregnant rabbit ovary', Endocrinology, vol. 124, no. 3, pp. 1198-1203.
Dharmarajan AM, Sueoka K, Miyazaki T, Atlas SJ, Ghodgaonkar RB, Dubin NH et al. Prostaglandins and progesterone secretion in the in vitro perfused pseudopregnant rabbit ovary. Endocrinology. 1989;124(3):1198-1203.
Dharmarajan, A. M. ; Sueoka, Kou ; Miyazaki, T. ; Atlas, S. J. ; Ghodgaonkar, R. B. ; Dubin, N. H. ; Zirkin, B. R. ; Wallach, E. E. / Prostaglandins and progesterone secretion in the in vitro perfused pseudopregnant rabbit ovary. In: Endocrinology. 1989 ; Vol. 124, No. 3. pp. 1198-1203.
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