Protective effects of blue light-blocking shades on phototoxicity in human ocular surface cells

Yoshimi Niwano, Atsuo Iwasawa, Kazuo Tsubota, Masahiko Ayaki, Kazuno Negishi

Research output: Contribution to journalArticle

Abstract

Objective Blue light hazards for retina and ocular surface have been repeatedly described and many protective methods are introduced for retina; however, no study has been conducted on ocular surface protection. The purpose of this in vitro study was to examine phototoxicity and shade protection after blue light irradiation in primary human cells of corneal surface origin. Methods and analysis Primary human cells of corneal surface origin were obtained from eye bank eyes. After blue light irradiation (405 nm) of these cells for 3 min, and a further 24 hours' incubation, surviving viable cells were assessed by the methyl thiazolyl tetrazolium assay. Simultaneously, cell viability was determined in wells covered by ultraviolet and blue light shades. Results Under subconfluent conditions, viable cells decreased by around 50% after blue light irradiation, compared with control cells without irradiation. The blue light phototoxicity was not blocked by the control shade, but the ultraviolet-blocking and blue light-blocking shades protected the cells from phototoxicity, producing a 30%-40% reduction (ultraviolet) and 15%-30% reduction (blue light) in viable cells. Conclusion These results indicate that blue light injures ocular surface cells and the cells are protected from damage by a shade. We recommend blue light protection to maintain ocular health, especially in high-risk populations, such as people with dry eye, contact lens users, the malnourished and the elderly.

Original languageEnglish
Article numbere000217
JournalBMJ Open Ophthalmology
Volume4
Issue number1
DOIs
Publication statusPublished - 2019 May 1

Fingerprint

Phototoxic Dermatitis
Light
Retina
Eye Banks
Crystalline Lens
Contact Lenses
Ultraviolet Rays
Cell Survival

Keywords

  • conjunctiva
  • cornea
  • ocular surface

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Protective effects of blue light-blocking shades on phototoxicity in human ocular surface cells. / Niwano, Yoshimi; Iwasawa, Atsuo; Tsubota, Kazuo; Ayaki, Masahiko; Negishi, Kazuno.

In: BMJ Open Ophthalmology, Vol. 4, No. 1, e000217, 01.05.2019.

Research output: Contribution to journalArticle

@article{5bddbe2b5ec64bf4a4c3724177bfb928,
title = "Protective effects of blue light-blocking shades on phototoxicity in human ocular surface cells",
abstract = "Objective Blue light hazards for retina and ocular surface have been repeatedly described and many protective methods are introduced for retina; however, no study has been conducted on ocular surface protection. The purpose of this in vitro study was to examine phototoxicity and shade protection after blue light irradiation in primary human cells of corneal surface origin. Methods and analysis Primary human cells of corneal surface origin were obtained from eye bank eyes. After blue light irradiation (405 nm) of these cells for 3 min, and a further 24 hours' incubation, surviving viable cells were assessed by the methyl thiazolyl tetrazolium assay. Simultaneously, cell viability was determined in wells covered by ultraviolet and blue light shades. Results Under subconfluent conditions, viable cells decreased by around 50{\%} after blue light irradiation, compared with control cells without irradiation. The blue light phototoxicity was not blocked by the control shade, but the ultraviolet-blocking and blue light-blocking shades protected the cells from phototoxicity, producing a 30{\%}-40{\%} reduction (ultraviolet) and 15{\%}-30{\%} reduction (blue light) in viable cells. Conclusion These results indicate that blue light injures ocular surface cells and the cells are protected from damage by a shade. We recommend blue light protection to maintain ocular health, especially in high-risk populations, such as people with dry eye, contact lens users, the malnourished and the elderly.",
keywords = "conjunctiva, cornea, ocular surface",
author = "Yoshimi Niwano and Atsuo Iwasawa and Kazuo Tsubota and Masahiko Ayaki and Kazuno Negishi",
year = "2019",
month = "5",
day = "1",
doi = "10.1136/bmjophth-2018-000217",
language = "English",
volume = "4",
journal = "BMJ Open Ophthalmology",
issn = "2397-3269",
publisher = "BMJ Publishing Group",
number = "1",

}

TY - JOUR

T1 - Protective effects of blue light-blocking shades on phototoxicity in human ocular surface cells

AU - Niwano, Yoshimi

AU - Iwasawa, Atsuo

AU - Tsubota, Kazuo

AU - Ayaki, Masahiko

AU - Negishi, Kazuno

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Objective Blue light hazards for retina and ocular surface have been repeatedly described and many protective methods are introduced for retina; however, no study has been conducted on ocular surface protection. The purpose of this in vitro study was to examine phototoxicity and shade protection after blue light irradiation in primary human cells of corneal surface origin. Methods and analysis Primary human cells of corneal surface origin were obtained from eye bank eyes. After blue light irradiation (405 nm) of these cells for 3 min, and a further 24 hours' incubation, surviving viable cells were assessed by the methyl thiazolyl tetrazolium assay. Simultaneously, cell viability was determined in wells covered by ultraviolet and blue light shades. Results Under subconfluent conditions, viable cells decreased by around 50% after blue light irradiation, compared with control cells without irradiation. The blue light phototoxicity was not blocked by the control shade, but the ultraviolet-blocking and blue light-blocking shades protected the cells from phototoxicity, producing a 30%-40% reduction (ultraviolet) and 15%-30% reduction (blue light) in viable cells. Conclusion These results indicate that blue light injures ocular surface cells and the cells are protected from damage by a shade. We recommend blue light protection to maintain ocular health, especially in high-risk populations, such as people with dry eye, contact lens users, the malnourished and the elderly.

AB - Objective Blue light hazards for retina and ocular surface have been repeatedly described and many protective methods are introduced for retina; however, no study has been conducted on ocular surface protection. The purpose of this in vitro study was to examine phototoxicity and shade protection after blue light irradiation in primary human cells of corneal surface origin. Methods and analysis Primary human cells of corneal surface origin were obtained from eye bank eyes. After blue light irradiation (405 nm) of these cells for 3 min, and a further 24 hours' incubation, surviving viable cells were assessed by the methyl thiazolyl tetrazolium assay. Simultaneously, cell viability was determined in wells covered by ultraviolet and blue light shades. Results Under subconfluent conditions, viable cells decreased by around 50% after blue light irradiation, compared with control cells without irradiation. The blue light phototoxicity was not blocked by the control shade, but the ultraviolet-blocking and blue light-blocking shades protected the cells from phototoxicity, producing a 30%-40% reduction (ultraviolet) and 15%-30% reduction (blue light) in viable cells. Conclusion These results indicate that blue light injures ocular surface cells and the cells are protected from damage by a shade. We recommend blue light protection to maintain ocular health, especially in high-risk populations, such as people with dry eye, contact lens users, the malnourished and the elderly.

KW - conjunctiva

KW - cornea

KW - ocular surface

UR - http://www.scopus.com/inward/record.url?scp=85066850662&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85066850662&partnerID=8YFLogxK

U2 - 10.1136/bmjophth-2018-000217

DO - 10.1136/bmjophth-2018-000217

M3 - Article

VL - 4

JO - BMJ Open Ophthalmology

JF - BMJ Open Ophthalmology

SN - 2397-3269

IS - 1

M1 - e000217

ER -