Protective effects of sodium-L-ascorbyl-2 phosphate on the development of UVB-induced damage in cultured mouse skin

Satomi Nayama, Makoto Takehana, Motoko Kanke, Shinobu Itoh, Eiji Ogata, Shizuko Kobayashi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The protective effect of sodium-L-ascorbyl-2 phosphate (As-2P), a stable form of ascorbic acid (AsA), against photodamage induced by a single dose of UVB exposure (290-320 nm, Max 312 nm) was investigated using cultured mouse skin. When the cultured skin was treated with various As-2P concentrations, the cutaneous AsA level increased in proportion to the As-2P concentration. After 3 h of incubation, the AsA level in the cultured skin treated with 2, 20 and 100 mM As-2P increased 1.03-, 2.17- and 6.27-fold, respectively, compared with that of the control skin. These results suggest that As-2P was transported into the cultured mouse skin where if was converted to AsA. After 3 h, the cutaneous AsA level in irradiated (20 kJ/m2) skin was depleted to a half of that in the control skin. However, the level in skin pretreated with 20 mM As-2P was maintained within normal limits, even after 24 h. Pretreatment with 20 mM As-2P significantly prevented such photodamage as sunburn cell formation, DNA fragmentation and lipid peroxidation, which were caused by a single dose of UVB irradiation. These results suggest that the protective effect of 20 mM As-2P on UVB-induced cutaneous damage is due to the maintenance of a normal As level by conversion of As-2P to As in skin tissue.

Original languageEnglish
Pages (from-to)1301-1305
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume22
Issue number12
DOIs
Publication statusPublished - 1999 Dec

Keywords

  • Cultured skin
  • DNA fragmentation
  • Lipid peroxidation
  • Sodium-L-ascorbyl-2 phosphate
  • Sunburn cell formation
  • UVB-irradiation

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Fingerprint Dive into the research topics of 'Protective effects of sodium-L-ascorbyl-2 phosphate on the development of UVB-induced damage in cultured mouse skin'. Together they form a unique fingerprint.

  • Cite this