Protective effects of sodium-L-ascorbyl-2 phosphate on the development of UVB-induced damage in cultured mouse skin

Satomi Nayama, Makoto Takehana, Motoko Kanke, Shinobu Itoh, Eiji Ogata, Shizuko Kobayashi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The protective effect of sodium-L-ascorbyl-2 phosphate (As-2P), a stable form of ascorbic acid (AsA), against photodamage induced by a single dose of UVB exposure (290-320 nm, Max 312 nm) was investigated using cultured mouse skin. When the cultured skin was treated with various As-2P concentrations, the cutaneous AsA level increased in proportion to the As-2P concentration. After 3 h of incubation, the AsA level in the cultured skin treated with 2, 20 and 100 mM As-2P increased 1.03-, 2.17- and 6.27-fold, respectively, compared with that of the control skin. These results suggest that As-2P was transported into the cultured mouse skin where if was converted to AsA. After 3 h, the cutaneous AsA level in irradiated (20 kJ/m2) skin was depleted to a half of that in the control skin. However, the level in skin pretreated with 20 mM As-2P was maintained within normal limits, even after 24 h. Pretreatment with 20 mM As-2P significantly prevented such photodamage as sunburn cell formation, DNA fragmentation and lipid peroxidation, which were caused by a single dose of UVB irradiation. These results suggest that the protective effect of 20 mM As-2P on UVB-induced cutaneous damage is due to the maintenance of a normal As level by conversion of As-2P to As in skin tissue.

Original languageEnglish
Pages (from-to)1301-1305
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume22
Issue number12
Publication statusPublished - 1999 Dec

Fingerprint

Sodium
Skin
Ascorbic Acid
ascorbate-2-phosphate
Sunburn
DNA Fragmentation
Lipid Peroxidation
Maintenance

Keywords

  • Cultured skin
  • DNA fragmentation
  • Lipid peroxidation
  • Sodium-L-ascorbyl-2 phosphate
  • Sunburn cell formation
  • UVB-irradiation

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Nayama, S., Takehana, M., Kanke, M., Itoh, S., Ogata, E., & Kobayashi, S. (1999). Protective effects of sodium-L-ascorbyl-2 phosphate on the development of UVB-induced damage in cultured mouse skin. Biological and Pharmaceutical Bulletin, 22(12), 1301-1305.

Protective effects of sodium-L-ascorbyl-2 phosphate on the development of UVB-induced damage in cultured mouse skin. / Nayama, Satomi; Takehana, Makoto; Kanke, Motoko; Itoh, Shinobu; Ogata, Eiji; Kobayashi, Shizuko.

In: Biological and Pharmaceutical Bulletin, Vol. 22, No. 12, 12.1999, p. 1301-1305.

Research output: Contribution to journalArticle

Nayama, S, Takehana, M, Kanke, M, Itoh, S, Ogata, E & Kobayashi, S 1999, 'Protective effects of sodium-L-ascorbyl-2 phosphate on the development of UVB-induced damage in cultured mouse skin', Biological and Pharmaceutical Bulletin, vol. 22, no. 12, pp. 1301-1305.
Nayama, Satomi ; Takehana, Makoto ; Kanke, Motoko ; Itoh, Shinobu ; Ogata, Eiji ; Kobayashi, Shizuko. / Protective effects of sodium-L-ascorbyl-2 phosphate on the development of UVB-induced damage in cultured mouse skin. In: Biological and Pharmaceutical Bulletin. 1999 ; Vol. 22, No. 12. pp. 1301-1305.
@article{f68893a9553047ffa4a6cefc11f8f256,
title = "Protective effects of sodium-L-ascorbyl-2 phosphate on the development of UVB-induced damage in cultured mouse skin",
abstract = "The protective effect of sodium-L-ascorbyl-2 phosphate (As-2P), a stable form of ascorbic acid (AsA), against photodamage induced by a single dose of UVB exposure (290-320 nm, Max 312 nm) was investigated using cultured mouse skin. When the cultured skin was treated with various As-2P concentrations, the cutaneous AsA level increased in proportion to the As-2P concentration. After 3 h of incubation, the AsA level in the cultured skin treated with 2, 20 and 100 mM As-2P increased 1.03-, 2.17- and 6.27-fold, respectively, compared with that of the control skin. These results suggest that As-2P was transported into the cultured mouse skin where if was converted to AsA. After 3 h, the cutaneous AsA level in irradiated (20 kJ/m2) skin was depleted to a half of that in the control skin. However, the level in skin pretreated with 20 mM As-2P was maintained within normal limits, even after 24 h. Pretreatment with 20 mM As-2P significantly prevented such photodamage as sunburn cell formation, DNA fragmentation and lipid peroxidation, which were caused by a single dose of UVB irradiation. These results suggest that the protective effect of 20 mM As-2P on UVB-induced cutaneous damage is due to the maintenance of a normal As level by conversion of As-2P to As in skin tissue.",
keywords = "Cultured skin, DNA fragmentation, Lipid peroxidation, Sodium-L-ascorbyl-2 phosphate, Sunburn cell formation, UVB-irradiation",
author = "Satomi Nayama and Makoto Takehana and Motoko Kanke and Shinobu Itoh and Eiji Ogata and Shizuko Kobayashi",
year = "1999",
month = "12",
language = "English",
volume = "22",
pages = "1301--1305",
journal = "Biological and Pharmaceutical Bulletin",
issn = "0918-6158",
publisher = "Pharmaceutical Society of Japan",
number = "12",

}

TY - JOUR

T1 - Protective effects of sodium-L-ascorbyl-2 phosphate on the development of UVB-induced damage in cultured mouse skin

AU - Nayama, Satomi

AU - Takehana, Makoto

AU - Kanke, Motoko

AU - Itoh, Shinobu

AU - Ogata, Eiji

AU - Kobayashi, Shizuko

PY - 1999/12

Y1 - 1999/12

N2 - The protective effect of sodium-L-ascorbyl-2 phosphate (As-2P), a stable form of ascorbic acid (AsA), against photodamage induced by a single dose of UVB exposure (290-320 nm, Max 312 nm) was investigated using cultured mouse skin. When the cultured skin was treated with various As-2P concentrations, the cutaneous AsA level increased in proportion to the As-2P concentration. After 3 h of incubation, the AsA level in the cultured skin treated with 2, 20 and 100 mM As-2P increased 1.03-, 2.17- and 6.27-fold, respectively, compared with that of the control skin. These results suggest that As-2P was transported into the cultured mouse skin where if was converted to AsA. After 3 h, the cutaneous AsA level in irradiated (20 kJ/m2) skin was depleted to a half of that in the control skin. However, the level in skin pretreated with 20 mM As-2P was maintained within normal limits, even after 24 h. Pretreatment with 20 mM As-2P significantly prevented such photodamage as sunburn cell formation, DNA fragmentation and lipid peroxidation, which were caused by a single dose of UVB irradiation. These results suggest that the protective effect of 20 mM As-2P on UVB-induced cutaneous damage is due to the maintenance of a normal As level by conversion of As-2P to As in skin tissue.

AB - The protective effect of sodium-L-ascorbyl-2 phosphate (As-2P), a stable form of ascorbic acid (AsA), against photodamage induced by a single dose of UVB exposure (290-320 nm, Max 312 nm) was investigated using cultured mouse skin. When the cultured skin was treated with various As-2P concentrations, the cutaneous AsA level increased in proportion to the As-2P concentration. After 3 h of incubation, the AsA level in the cultured skin treated with 2, 20 and 100 mM As-2P increased 1.03-, 2.17- and 6.27-fold, respectively, compared with that of the control skin. These results suggest that As-2P was transported into the cultured mouse skin where if was converted to AsA. After 3 h, the cutaneous AsA level in irradiated (20 kJ/m2) skin was depleted to a half of that in the control skin. However, the level in skin pretreated with 20 mM As-2P was maintained within normal limits, even after 24 h. Pretreatment with 20 mM As-2P significantly prevented such photodamage as sunburn cell formation, DNA fragmentation and lipid peroxidation, which were caused by a single dose of UVB irradiation. These results suggest that the protective effect of 20 mM As-2P on UVB-induced cutaneous damage is due to the maintenance of a normal As level by conversion of As-2P to As in skin tissue.

KW - Cultured skin

KW - DNA fragmentation

KW - Lipid peroxidation

KW - Sodium-L-ascorbyl-2 phosphate

KW - Sunburn cell formation

KW - UVB-irradiation

UR - http://www.scopus.com/inward/record.url?scp=0033373423&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033373423&partnerID=8YFLogxK

M3 - Article

VL - 22

SP - 1301

EP - 1305

JO - Biological and Pharmaceutical Bulletin

JF - Biological and Pharmaceutical Bulletin

SN - 0918-6158

IS - 12

ER -