Protective efficacy of a hydroxy fatty acid against gastric Helicobacter infections

Hidenori Matsui, Tetsufumi Takahashi, Somay Y. Murayama, Marina Kawaguchi, Koichi Matsuo, Masahiko Nakamura

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: We have previously revealed that omega-3 polyunsaturated fatty acids can prevent Helicobacter pylori infection by blocking the futalosine pathway, an alternative route for menaquinone (MK) biosynthesis. Materials and Methods: 1, Different H. pylori strains were grown in liquid media supplemented with linoleic acid, an omega-6 fatty acid, or its 10-hydroxy derivative, 10-hydroxy-cis-12-octadecenoic acid (HYA), in the presence or absence of MK. The bacterial numbers in the media were estimated by plating; 2, C57BL/6NCrl mice received drinking water supplemented with different fatty acids starting from 1 week before infection with H. pylori or Helicobacter suis until the end of the experiment. The gastric colonization levels of H. pylori or H. suis were determined 2 weeks after infection by plating or quantitative PCR, respectively; 3, Mice were given HYA, starting 1 week before infection with H. suis and continuing until 6 months after infection, for analysis of the gastric conditions. Results: 1, A low concentration (20 μmol/L) of HYA in culture broth suppressed the growth of H. pylori, and this inhibition was reduced by MK supplementation; 2, HYA treatment protected mice against H. pylori or H. suis infection; 3, HYA treatment suppressed the formation of lymphoid follicles in the gastric mucus layer after H. suis infection. Conclusions: HYA prevents gastric Helicobacter infections by blocking their futalosine pathways. Daily HYA supplementation is effective for the prevention of gastric mucosa-associated lymphoid tissue lymphoma induced by persistent infection with H. suis.

Original languageEnglish
Article numbere12430
JournalHelicobacter
Volume22
Issue number6
DOIs
Publication statusPublished - 2017 Dec 1

Fingerprint

Hydroxy Acids
Helicobacter Infections
Stomach
Pylorus
Fatty Acids
Infection
Helicobacter heilmannii
Omega-6 Fatty Acids
Vitamin K 2
Marginal Zone B-Cell Lymphoma
Omega-3 Fatty Acids
Linoleic Acid
Mucus
Gastric Mucosa
12-octadecenoic acid
Unsaturated Fatty Acids
Inbred C57BL Mouse
Helicobacter pylori
Drinking Water
Polymerase Chain Reaction

Keywords

  • alternative menaquinone biosynthesis
  • Helicobacter suis
  • hydroxy fatty acid
  • mucosa-associated lymphoid tissue lymphoma

ASJC Scopus subject areas

  • Gastroenterology
  • Infectious Diseases

Cite this

Matsui, H., Takahashi, T., Murayama, S. Y., Kawaguchi, M., Matsuo, K., & Nakamura, M. (2017). Protective efficacy of a hydroxy fatty acid against gastric Helicobacter infections. Helicobacter, 22(6), [e12430]. https://doi.org/10.1111/hel.12430

Protective efficacy of a hydroxy fatty acid against gastric Helicobacter infections. / Matsui, Hidenori; Takahashi, Tetsufumi; Murayama, Somay Y.; Kawaguchi, Marina; Matsuo, Koichi; Nakamura, Masahiko.

In: Helicobacter, Vol. 22, No. 6, e12430, 01.12.2017.

Research output: Contribution to journalArticle

Matsui, H, Takahashi, T, Murayama, SY, Kawaguchi, M, Matsuo, K & Nakamura, M 2017, 'Protective efficacy of a hydroxy fatty acid against gastric Helicobacter infections', Helicobacter, vol. 22, no. 6, e12430. https://doi.org/10.1111/hel.12430
Matsui, Hidenori ; Takahashi, Tetsufumi ; Murayama, Somay Y. ; Kawaguchi, Marina ; Matsuo, Koichi ; Nakamura, Masahiko. / Protective efficacy of a hydroxy fatty acid against gastric Helicobacter infections. In: Helicobacter. 2017 ; Vol. 22, No. 6.
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AU - Takahashi, Tetsufumi

AU - Murayama, Somay Y.

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AU - Matsuo, Koichi

AU - Nakamura, Masahiko

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N2 - Background: We have previously revealed that omega-3 polyunsaturated fatty acids can prevent Helicobacter pylori infection by blocking the futalosine pathway, an alternative route for menaquinone (MK) biosynthesis. Materials and Methods: 1, Different H. pylori strains were grown in liquid media supplemented with linoleic acid, an omega-6 fatty acid, or its 10-hydroxy derivative, 10-hydroxy-cis-12-octadecenoic acid (HYA), in the presence or absence of MK. The bacterial numbers in the media were estimated by plating; 2, C57BL/6NCrl mice received drinking water supplemented with different fatty acids starting from 1 week before infection with H. pylori or Helicobacter suis until the end of the experiment. The gastric colonization levels of H. pylori or H. suis were determined 2 weeks after infection by plating or quantitative PCR, respectively; 3, Mice were given HYA, starting 1 week before infection with H. suis and continuing until 6 months after infection, for analysis of the gastric conditions. Results: 1, A low concentration (20 μmol/L) of HYA in culture broth suppressed the growth of H. pylori, and this inhibition was reduced by MK supplementation; 2, HYA treatment protected mice against H. pylori or H. suis infection; 3, HYA treatment suppressed the formation of lymphoid follicles in the gastric mucus layer after H. suis infection. Conclusions: HYA prevents gastric Helicobacter infections by blocking their futalosine pathways. Daily HYA supplementation is effective for the prevention of gastric mucosa-associated lymphoid tissue lymphoma induced by persistent infection with H. suis.

AB - Background: We have previously revealed that omega-3 polyunsaturated fatty acids can prevent Helicobacter pylori infection by blocking the futalosine pathway, an alternative route for menaquinone (MK) biosynthesis. Materials and Methods: 1, Different H. pylori strains were grown in liquid media supplemented with linoleic acid, an omega-6 fatty acid, or its 10-hydroxy derivative, 10-hydroxy-cis-12-octadecenoic acid (HYA), in the presence or absence of MK. The bacterial numbers in the media were estimated by plating; 2, C57BL/6NCrl mice received drinking water supplemented with different fatty acids starting from 1 week before infection with H. pylori or Helicobacter suis until the end of the experiment. The gastric colonization levels of H. pylori or H. suis were determined 2 weeks after infection by plating or quantitative PCR, respectively; 3, Mice were given HYA, starting 1 week before infection with H. suis and continuing until 6 months after infection, for analysis of the gastric conditions. Results: 1, A low concentration (20 μmol/L) of HYA in culture broth suppressed the growth of H. pylori, and this inhibition was reduced by MK supplementation; 2, HYA treatment protected mice against H. pylori or H. suis infection; 3, HYA treatment suppressed the formation of lymphoid follicles in the gastric mucus layer after H. suis infection. Conclusions: HYA prevents gastric Helicobacter infections by blocking their futalosine pathways. Daily HYA supplementation is effective for the prevention of gastric mucosa-associated lymphoid tissue lymphoma induced by persistent infection with H. suis.

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