Protective efficacy of a hydroxy fatty acid against gastric Helicobacter infections

Hidenori Matsui, Tetsufumi Takahashi, Somay Y. Murayama, Marina Kawaguchi, Koichi Matsuo, Masahiko Nakamura

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


Background: We have previously revealed that omega-3 polyunsaturated fatty acids can prevent Helicobacter pylori infection by blocking the futalosine pathway, an alternative route for menaquinone (MK) biosynthesis. Materials and Methods: 1, Different H. pylori strains were grown in liquid media supplemented with linoleic acid, an omega-6 fatty acid, or its 10-hydroxy derivative, 10-hydroxy-cis-12-octadecenoic acid (HYA), in the presence or absence of MK. The bacterial numbers in the media were estimated by plating; 2, C57BL/6NCrl mice received drinking water supplemented with different fatty acids starting from 1 week before infection with H. pylori or Helicobacter suis until the end of the experiment. The gastric colonization levels of H. pylori or H. suis were determined 2 weeks after infection by plating or quantitative PCR, respectively; 3, Mice were given HYA, starting 1 week before infection with H. suis and continuing until 6 months after infection, for analysis of the gastric conditions. Results: 1, A low concentration (20 μmol/L) of HYA in culture broth suppressed the growth of H. pylori, and this inhibition was reduced by MK supplementation; 2, HYA treatment protected mice against H. pylori or H. suis infection; 3, HYA treatment suppressed the formation of lymphoid follicles in the gastric mucus layer after H. suis infection. Conclusions: HYA prevents gastric Helicobacter infections by blocking their futalosine pathways. Daily HYA supplementation is effective for the prevention of gastric mucosa-associated lymphoid tissue lymphoma induced by persistent infection with H. suis.

Original languageEnglish
Article numbere12430
Issue number6
Publication statusPublished - 2017 Dec


  • Helicobacter suis
  • alternative menaquinone biosynthesis
  • hydroxy fatty acid
  • mucosa-associated lymphoid tissue lymphoma

ASJC Scopus subject areas

  • Gastroenterology
  • Infectious Diseases


Dive into the research topics of 'Protective efficacy of a hydroxy fatty acid against gastric Helicobacter infections'. Together they form a unique fingerprint.

Cite this