Proteolipid protein 2 is associated with melanoma metastasis

Yoshiko Sonoda; Masako Warita; Takaharu Suzuki; Hiroki Ozawa; Yusuke Fukuda; Megumi Funakoshi-Tago; Tadashi Kasahara

doi:10.3892/or-00000645

Proteolipid protein 2 is associated with melanoma metastasis

Yoshiko Sonoda, Masako Warita, Takaharu Suzuki, Hiroki Ozawa, Yusuke Fukuda, Megumi Funakoshi-Tago, Tadashi Kasahara

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

The metastasis of malignant tumor cells from the primary tumor to distant sites in the body is a complex process. To identify genes that may be essential for metastasis, we established poorly metastatic mouse melanoma cells, namely Y925F-mutated FAK-transfected cells (Y925F cells), from the highly metastatic mouse melanoma cell line B16F10, and performed expression analyses. The expression of phospholipid protein 2 (PLP2) was markedly down-regulated in the Y925F cells. To elucidate the function of PLP2, we established melanoma cells overexpressing PLP2. We found that PLP2 enhanced proliferation, adhesion, invasion, and MMP-2 secretion in vitro, and tumor metastasis in vivo. These results suggest that PLP2 aids metastasis. Furthermore, we showed that PLP2 binds specifically to PI3K, thus activating Akt.

Original language	English
Pages (from-to)	371-376
Number of pages	6
Journal	Oncology reports
Volume	23
Issue number	2
DOIs	https://doi.org/10.3892/or-00000645
Publication status	Published - 2010 Feb 1

Keywords

Akt
FAK
Melanoma
Metastasis
PI3K
PLP2

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3892/or-00000645

Cite this

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title = "Proteolipid protein 2 is associated with melanoma metastasis",

abstract = "The metastasis of malignant tumor cells from the primary tumor to distant sites in the body is a complex process. To identify genes that may be essential for metastasis, we established poorly metastatic mouse melanoma cells, namely Y925F-mutated FAK-transfected cells (Y925F cells), from the highly metastatic mouse melanoma cell line B16F10, and performed expression analyses. The expression of phospholipid protein 2 (PLP2) was markedly down-regulated in the Y925F cells. To elucidate the function of PLP2, we established melanoma cells overexpressing PLP2. We found that PLP2 enhanced proliferation, adhesion, invasion, and MMP-2 secretion in vitro, and tumor metastasis in vivo. These results suggest that PLP2 aids metastasis. Furthermore, we showed that PLP2 binds specifically to PI3K, thus activating Akt.",

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author = "Yoshiko Sonoda and Masako Warita and Takaharu Suzuki and Hiroki Ozawa and Yusuke Fukuda and Megumi Funakoshi-Tago and Tadashi Kasahara",

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T1 - Proteolipid protein 2 is associated with melanoma metastasis

AU - Sonoda, Yoshiko

AU - Warita, Masako

AU - Suzuki, Takaharu

AU - Ozawa, Hiroki

AU - Fukuda, Yusuke

AU - Funakoshi-Tago, Megumi

AU - Kasahara, Tadashi

PY - 2010/2/1

Y1 - 2010/2/1

N2 - The metastasis of malignant tumor cells from the primary tumor to distant sites in the body is a complex process. To identify genes that may be essential for metastasis, we established poorly metastatic mouse melanoma cells, namely Y925F-mutated FAK-transfected cells (Y925F cells), from the highly metastatic mouse melanoma cell line B16F10, and performed expression analyses. The expression of phospholipid protein 2 (PLP2) was markedly down-regulated in the Y925F cells. To elucidate the function of PLP2, we established melanoma cells overexpressing PLP2. We found that PLP2 enhanced proliferation, adhesion, invasion, and MMP-2 secretion in vitro, and tumor metastasis in vivo. These results suggest that PLP2 aids metastasis. Furthermore, we showed that PLP2 binds specifically to PI3K, thus activating Akt.

AB - The metastasis of malignant tumor cells from the primary tumor to distant sites in the body is a complex process. To identify genes that may be essential for metastasis, we established poorly metastatic mouse melanoma cells, namely Y925F-mutated FAK-transfected cells (Y925F cells), from the highly metastatic mouse melanoma cell line B16F10, and performed expression analyses. The expression of phospholipid protein 2 (PLP2) was markedly down-regulated in the Y925F cells. To elucidate the function of PLP2, we established melanoma cells overexpressing PLP2. We found that PLP2 enhanced proliferation, adhesion, invasion, and MMP-2 secretion in vitro, and tumor metastasis in vivo. These results suggest that PLP2 aids metastasis. Furthermore, we showed that PLP2 binds specifically to PI3K, thus activating Akt.

KW - Akt

KW - FAK

KW - Melanoma

KW - Metastasis

KW - PI3K

KW - PLP2

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Proteolipid protein 2 is associated with melanoma metastasis

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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