Proteome analysis of focal adhesion kinase (FAK)-overexpressing cells

Ryoko Utsubo, Yoshiko Sonoda, Reiko Takahashi, Shiro Iijima, Eriko Aizu-Yokota, Tadashi Kasahara

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

We established several focal adhesion kinase (FAK) cDNA-transfected cells and found that FAK-transfected HL-60 (HL-60/FAK) cells are resistant to apoptosis induced with hydrogen peroxide, etoposide and radiation compared with the parental HL-60 or the vector-transfected (HL-60/Vect) cells. We carried out proteome analysis to study the mechanism of resistance to apoptosis in HL-60/FAK cells. Among 300 spots resolved in two-dimensional gels, ca. 10% of them were significantly increased in HL-60/FAK cells compared with HL-60/Vect cells, whereas ca. 2% of them were decreased or disappeared. These proteins were performed for further analysis by Western blots or N-terminal sequencing or mass spectrometry. Increased proteins included stress proteins such as hsp90, ribosomal proteins, and antioxidant enzymes such as peroxyredoxin 2. Some of these proteins are assumed to contribute to the antiapoptotic action of FAK.

Original languageEnglish
Pages (from-to)1735-1741
Number of pages7
JournalBiological and Pharmaceutical Bulletin
Volume27
Issue number11
DOIs
Publication statusPublished - 2004 Nov

Keywords

  • Focal adhesion kinase (FAK)
  • Peroxyredoxin
  • Proteome analysis
  • Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE)

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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