Proteome expression database of ewing sarcoma: A segment of the genome medicine database of Japan proteomics

Kazutaka Kikuta, Yukako Tsunehiro, Akihiko Yoshida, Naobumi Tochigi, Setsuo Hirohahsi, Akira Kawai, Tadashi Kondo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Ewing sarcoma is the second most common primary malignant bone tumor in children and adolescents worldwide. Here, we report an open-access proteome expression database of eight Ewing sarcoma cases using proteome data obtained by two-dimensional difference gel electrophoresis (2D-DIGE) and mass spectrometry. Proteins extracted from primary tumor tissues were labeled with CyDye DIGE Fluor saturation dye, and separated using a large format electrophoresis device, generating 2431 protein spots. Mass spectrometry following in-gel digestion identified 330 protein spots corresponding to 220 proteins. Multiple proteins were observed from single protein spots, and single proteins generated multiple protein spots, suggesting diversity of the proteome observed by 2D-DIGE. The results of 2D-DIGE and protein identification by mass spectrometry, and part of the corresponding clinico-pathological data such as prognosis after treatments are freely accessible in the public proteome database Genome Medicine Database of Japan Proteomics (GeMDBJ Proteomics, https://gemdbj.nibio.go.jp/dgdb/DigeTop.do).

Original languageEnglish
Pages (from-to)500-504
Number of pages5
JournalJournal of Proteomics and Bioinformatics
Volume2
Issue number12
DOIs
Publication statusPublished - 2009 Dec 1

Keywords

  • Ewing sarcoma proteomics
  • Gemdbj proteomics
  • Mass spectrometry
  • Proteome database
  • Two-dimensional difference gel electrophoresis (2d-dige)

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Computer Science Applications
  • Cell Biology

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