Proteomic analysis of α-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor complexes

Myoung Goo Kang, Mutsuo Nuriya, Yurong Guo, Kevin D. Martindale, Daniel Z. Lee, Richard L. Huganir

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The AMPA receptor (AMPA-R) is a major excitatory neurotransmitter receptor in the brain. Identifying and characterizing the neuronal proteins interacting with AMPA-Rs have provided important information about the molecular mechanisms underlying synaptic transmission and plasticity. In this study, to identify more AMPA-R interactors in vivo, we performed proteomic analyses of AMPA-R complexes from the brain.AMPA-Rcomplexes were isolated from the brain through various combinations of biochemical techniques for solubilization, enrichment, and immunoprecipitation. Mass spectrometry analyses of these isolated complexes identified several novel components of the AMPA-R complexes as well as some previously identified components. The identification of these novel components helps to further define the complex mechanisms involved in the regulation of AMPA receptor function and synaptic plasticity.

Original languageEnglish
Pages (from-to)28632-28645
Number of pages14
JournalJournal of Biological Chemistry
Volume287
Issue number34
DOIs
Publication statusPublished - 2012 Aug 17

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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