Proteomic analysis of plasma proteins in Japanese semisuper centenarians

Yuri Miura, Yuji Sato, Yasumichi Arai, Yukiko Abe, Michiyo Takayama, Tosifusa Toda, Nobuyoshi Hirose, Tamao Endo

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We performed proteomic analysis of plasma proteins in Japanese semisuper centenarians (SSCs) (>105years) and young controls (20-39years), and found that 18 protein spots were altered in the plasma of SSCs. From peptide mass fingerprinting following in-gel digestion, it was demonstrated that paraoxonase/arylesterase 1 (PON 1) and apolipoprotein E were decreased, while haptoglobin β-chain, α1-microglobulin, and clusterin precursor were increased in SSCs. Interestingly, proteins related to oxidative stress, PON1, haptoglobin, α1-microglobulin, and clusterin, were altered in SSCs. These results suggest that systemic redox regulation is important for the longevity of SSCs. Overall, proteomics analysis is a powerful technique to search for useful biomarkers for future studies in gerontology and to characterize the individual proteins associated with successful aging of SSCs.

Original languageEnglish
Pages (from-to)81-85
Number of pages5
JournalExperimental Gerontology
Volume46
Issue number1
DOIs
Publication statusPublished - 2011 Jan

Fingerprint

Proteomics
Clusterin
Blood Proteins
Haptoglobins
Geriatrics
Aryldialkylphosphatase
Proteins
Oxidative stress
Biomarkers
Aging of materials
Gels
Plasmas
Peptides
Peptide Mapping
Apolipoproteins E
Oxidation-Reduction
Digestion
Oxidative Stress
apolipoprotein E1
arylesterase

Keywords

  • Aging
  • Oxidative stress
  • Plasma proteins
  • Proteomics
  • Semisuper centenarian

ASJC Scopus subject areas

  • Ageing
  • Biochemistry
  • Cell Biology
  • Endocrinology
  • Genetics
  • Molecular Biology

Cite this

Proteomic analysis of plasma proteins in Japanese semisuper centenarians. / Miura, Yuri; Sato, Yuji; Arai, Yasumichi; Abe, Yukiko; Takayama, Michiyo; Toda, Tosifusa; Hirose, Nobuyoshi; Endo, Tamao.

In: Experimental Gerontology, Vol. 46, No. 1, 01.2011, p. 81-85.

Research output: Contribution to journalArticle

Miura, Yuri ; Sato, Yuji ; Arai, Yasumichi ; Abe, Yukiko ; Takayama, Michiyo ; Toda, Tosifusa ; Hirose, Nobuyoshi ; Endo, Tamao. / Proteomic analysis of plasma proteins in Japanese semisuper centenarians. In: Experimental Gerontology. 2011 ; Vol. 46, No. 1. pp. 81-85.
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