Proto-oncogene expression in three human hepatoma cell lines, HCC-M, HCC-T and PLC/PRF/5.

Hidetsugu Saito, T. Morizane, T. Watanabe, T. Kagawa, S. Miyaguchi, N. Kumagai, K. Tsuchimoto, M. Tsuchiya

Research output: Contribution to journalArticle

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Abstract

Changes of nucleotide sequences and expressions of cellular oncogenes in human hepatoma cell lines, PLC/PRF/5, HCC-M and HCC-T cells, were examined by Southern and Northern blot analyses. The probes used are DNA fragment of myc, N-, H-, K-ras, fos, fms, raf, erb-A, erb-B, and erb-B2 genes and synthetic oligonucleotides corresponding to the part of N-, H-, K-ras genes. The results are as follows. DNA amplification and rearrangement were not detected in these three human hepatoma cell lines. Point mutations at codons 12, 13, and 61 in N- and K-ras genes were not demonstrated in these cell lines. N-, H-, K-ras and myc transcripts were detected in these three cell lines. However, fos gene transcript was detected only in PLC/PRF/5 and HCC-M cells which were derived from hepatitis B related hepatocellular carcinoma and having integrated hepatitis B virus (HBV) DNA. These data showed that there are no specific proto-oncogene expression into RNA except for myc and ras genes, nor DNA rearrangement in these 3 human hepatoma cell lines with regards to at least 10 different oncogenes examined and suggest the relationship between fos gene expression and integration of HBV DNA in host cell DNA.

Original languageEnglish
Pages (from-to)139-145
Number of pages7
JournalKeio Journal of Medicine
Volume40
Issue number3
Publication statusPublished - 1991 Sep

Fingerprint

ras Genes
Proto-Oncogenes
Hepatocellular Carcinoma
Cell Line
fos Genes
Gene Rearrangement
Oncogenes
Hepatitis B virus
DNA
Synthetic Genes
myc Genes
DNA Probes
Southern Blotting
Hepatitis B
Point Mutation
Codon
Oligonucleotides
Northern Blotting
RNA
T-Lymphocytes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Saito, H., Morizane, T., Watanabe, T., Kagawa, T., Miyaguchi, S., Kumagai, N., ... Tsuchiya, M. (1991). Proto-oncogene expression in three human hepatoma cell lines, HCC-M, HCC-T and PLC/PRF/5. Keio Journal of Medicine, 40(3), 139-145.

Proto-oncogene expression in three human hepatoma cell lines, HCC-M, HCC-T and PLC/PRF/5. / Saito, Hidetsugu; Morizane, T.; Watanabe, T.; Kagawa, T.; Miyaguchi, S.; Kumagai, N.; Tsuchimoto, K.; Tsuchiya, M.

In: Keio Journal of Medicine, Vol. 40, No. 3, 09.1991, p. 139-145.

Research output: Contribution to journalArticle

Saito, H, Morizane, T, Watanabe, T, Kagawa, T, Miyaguchi, S, Kumagai, N, Tsuchimoto, K & Tsuchiya, M 1991, 'Proto-oncogene expression in three human hepatoma cell lines, HCC-M, HCC-T and PLC/PRF/5.', Keio Journal of Medicine, vol. 40, no. 3, pp. 139-145.
Saito H, Morizane T, Watanabe T, Kagawa T, Miyaguchi S, Kumagai N et al. Proto-oncogene expression in three human hepatoma cell lines, HCC-M, HCC-T and PLC/PRF/5. Keio Journal of Medicine. 1991 Sep;40(3):139-145.
Saito, Hidetsugu ; Morizane, T. ; Watanabe, T. ; Kagawa, T. ; Miyaguchi, S. ; Kumagai, N. ; Tsuchimoto, K. ; Tsuchiya, M. / Proto-oncogene expression in three human hepatoma cell lines, HCC-M, HCC-T and PLC/PRF/5. In: Keio Journal of Medicine. 1991 ; Vol. 40, No. 3. pp. 139-145.
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