Protocol for enhanced proliferation of human pluripotent stem cells in tryptophan-fortified media

Kotaro Kameda, Shota Someya, Jun Fujita, Keiichi Fukuda, Shugo Tohyama

Research output: Contribution to journalArticlepeer-review

Abstract

We describe a protocol for the efficient culture of human pluripotent stem cells (hPSCs) by supplementing conventional culture medium with L-tryptophan (TRP). TRP is an essential amino acid that is widely available at an affordable cost, thereby allowing cost-effective proliferation of hPSCs compared to using a conventional medium alone. Here, we describe the steps for enhanced proliferation of hPSCs from dermal fibroblasts or peripheral blood cells, but the protocol can be applied to any hPSCs. For complete details on the use and execution of this protocol, please refer to Someya et al. (2021).

Original languageEnglish
Article number101341
JournalSTAR Protocols
Volume3
Issue number2
DOIs
Publication statusPublished - 2022 Jun 17

Keywords

  • Cell culture
  • Metabolism
  • Stem Cells

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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