@article{90e61458f4cc4e6db34b45719ac1d1d2,
title = "Protocol to image deuterated propofol in living rat neurons using multimodal stimulated Raman scattering microscopy",
abstract = "Propofol is a widely used anesthetic important in clinics, but like many other bioactive molecules, it is too small to be tagged and visualized by fluorescent dyes. Here, we present a protocol to visualize deuterated propofol in living rat neurons using stimulated Raman scattering (SRS) microscopy with carbon-deuterium bonds serving as a Raman tag. We describe the preparation and culture of rat neurons, followed by optimization of the SRS system. We then detail neuron loading and real-time imaging of anesthesia dynamics. For complete details on the use and execution of this protocol, please refer to Oda et al.1",
keywords = "Cell Biology, Cell Culture, Microscopy, Molecular/Chemical Probes, Neuroscience",
author = "Wenying Zhong and Robert Oda and Yasuyuki Ozeki and Masato Yasui and Mutsuo Nuriya",
note = "Funding Information: We would like to thank Evident Corporation for technical assistance. Funding was provided by JST (Japan Science and Technology Agency) PRESTO ( JPMJPR17G6 ), CREST ( JPMJCR1872 ), Mirai Program ( JPMJMI22G5 ), Japan Society for the Promotion of Science Grant-in-Aid for Scientific Research (JSPS KAKENHI) ( 20K20593 , 20H02881 ), and the Nakatani Foundation . Funding Information: We would like to thank Evident Corporation for technical assistance. Funding was provided by JST (Japan Science and Technology Agency) PRESTO (JPMJPR17G6), CREST (JPMJCR1872), Mirai Program (JPMJMI22G5), Japan Society for the Promotion of Science Grant-in-Aid for Scientific Research (JSPS KAKENHI) (20K20593, 20H02881), and the Nakatani Foundation. W.Z. R.O. and M.N. conceived of the project, and M.N. supervised the project with the help of M.Y. and Y.O. W.Z. performed cell culture, imaging of neurons, and data analysis. W.Z. and M.N. wrote the manuscript with the help of R.O. M.Y. and Y.O. R.O. is an employee of and holds stock options in Chordia Therapeutics Inc. Publisher Copyright: {\textcopyright} 2023 The Author(s)",
year = "2023",
month = jun,
day = "16",
doi = "10.1016/j.xpro.2023.102221",
language = "English",
volume = "4",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",
}