Protoplast fusion method for efficient transformation of mouse spermatogenic cells

Mitsuru Jimbo, Midori Matsumoto, Shun Ichi Kurata, Motonori Hoshi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The efficiencies of methods for introducing DNAs into spermatogenic cells were investigat ed. The calcium phosphate method has been most commonly used to transfect DNA into cultured cells, but this method severely damages spermatogenic cells. Therefore, we investigated the efficiencies of transfection of spermatogenic cells by DEAE-dextran, modified liposome, and protoplast fusion methods, which seemed to cause less damage to spermatogenic cells than the calcium phosphate method. The protoplast fusion method was the most suitable for transfection of spermatogenic cells.

Original languageEnglish
Pages (from-to)321-323
Number of pages3
JournalJournal of Biochemistry
Volume117
Issue number2
DOIs
Publication statusPublished - 1995 Feb
Externally publishedYes

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Keywords

  • Germ cell
  • Protoplast fusion method
  • Spermatogenesis
  • Transfection

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

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