Psychotropic drugs and local anesthetics restore cardiolipin-inhibited mammalian DNA topoisomerase I activity

Gulzar Hussain Shah, Kazuhiro Kataoka, Akiko Ueno, Yoshihisa Ohtsuka, Tohru Mizushima, Kazuhisa Sekimizu

Research output: Contribution to journalArticle


The activity of mammalian topoisomerase I is inhibited by acidic phospholipids. We investigated the effect of psychotropic drugs and local anesthetics on cardiolipin-inhibited calf thymus DNA topoisomerase I activity. Chlorpromazine, promethazine, and imipramine, which interact with phospholipids, suppressed the inhibitory effect of cardiolipin. The present results suggest that relaxation of DNA supercoiling is involved in the cytotoxic action of these drugs.

Original languageEnglish
Pages (from-to)1233-1234
Number of pages2
JournalBiological and Pharmaceutical Bulletin
Issue number12
Publication statusPublished - 1997 Jan 1



  • Acidic phospholipid
  • Cardiolipin
  • Chlorpromazine
  • Cytotoxicity
  • DNA topoisomerase 1

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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