PTK2 rs7460 and rs7843014 polymorphisms and exceptional longevity: A functional replication study

Nuria Garatachea, Noriyuki Fuku, Zi Hong He, Ye Tian, Yasumichi Arai, Yukiko Abe, Haruka Murakami, Motohiko Miyachi, Thomas Yvert, Letizia Venturini, Catalina Santiago, Alejandro Santos-Lozano, Gabriel Rodríguez, Giovanni Ricevuti, Helios Pareja-Galeano, Fabian Sanchis-Gomar, Enzo Emanuele, Nobuyoshi Hirose, Alejandro Lucia

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Focal adhesion is critical for cell survival. The focal adhesion kinase (FAK, or PTK2) is an important component of the human interactome and thus is a potential longevity-related protein. Here we studied the association between two PTK2 gene single-nucleotide polymorphisms (SNPs) (rs7843014, rs7460) and exceptional longevity (EL). In addition to gaining insight into their functionality by determining luciferase gene reporter activity, we studied the genotype/allele frequency of these two SNPs among three different cohorts: (1) Spanish centenarians (n = 175, 100-111 years, 144 women) and healthy controls (n = 355, 20-50 years, 284 women); (2) Italian centenarians (n = 79, 100-104 years, 40 women) and controls (n = 316, 29-50 years, 156 women); and (3) Japanese centenarians (n = 742, 100-116 years, 623 women) and healthy controls (n = 499, 23-59 years, 356 women). Both SNPs had functional significance, with the A allele up-regulating luciferase activity compared to the other allele (rs7460 T allele and rs7843014 C allele, respectively). The A allele of both SNPs was negatively associated with EL in the Spanish cohort (rs7460, odds ratio [OR] adjusted by sex = 0.40, 95% confidence intervals [CI] 0.3, 0.6, p < 0.001); rs7843014, OR = 0.37, 95% CI 0.3, 0.5, p < 0.001). The OR of being a centenarian if having the rs7460-TT genotype was 6.68 (95% CI 4.1, 10.8, p < 0.001). The rs7843014 CC genotype was also positively associated with EL (OR = 7.58, 95% CI 4.6, 12.3, p < 0.001]. No association was, however, found for the Italian or Japanese cohorts. Thus, two genotypes of the FAK gene, rs7460 TT and rs7843014 CC, are possibly associated with lower gene expression and might favor the likelihood of reaching EL in the Spanish population. Further research is needed to unveil the mechanisms by which FAK expression could perhaps influence the rate of aging.

Original languageEnglish
Pages (from-to)430-438
Number of pages9
JournalRejuvenation Research
Volume17
Issue number5
DOIs
Publication statusPublished - 2014 Oct 1

ASJC Scopus subject areas

  • Ageing
  • Geriatrics and Gerontology

Fingerprint Dive into the research topics of 'PTK2 rs7460 and rs7843014 polymorphisms and exceptional longevity: A functional replication study'. Together they form a unique fingerprint.

Cite this