Pulmonary dysfunction in neonatal SP-B-deficient mice

Keisuke Tokieda, Jeffrey A. Whitsett, Jean C. Clark, Timothy E. Weaver, Kazushige Ikeda, Keith B. McConnell, Alan H. Jobe, Machiko Ikegami, Harriet S. Iwamoto

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Pulmonary function was assessed in newborn wild-type and homozygous and heterozygous surfactant protein B (SP-B)-deficient mice after birth. SP-B +/+ and SP-B+/- mice became well oxygenated and survived postnatally. Although lung compliance was decreased slightly in the SP-B+/- mice, lung volumes and compliances were decreased markedly in homozygous SP-B-/- mice. They died rapidly after birth, failing to inflate their lungs or oxygenate. SP-B proprotein was absent in the SP-B-/- mice and was reduced in the SP-B+/- mice, as assessed by Western analysis. Surfactant protein A, surfactant proprotein C, surfactant protein D, and surfactant phospholipid content in lungs from SP-B+/- and SP-B-/- mice were not altered. Lung saturated phosphatidylcholine and precursor incorporation into saturated phosphatidylcholine were not influenced by SP-B genotype. Intratracheal administration of perfluorocarbon resulted in lung expansion, oxygenation, and prolonged survival of SP-B-/- mice and in reduced lung compliance in SP- B+/+ and SP-B+/- mice. Lack of SP-B caused respiratory failure at birth, and decreased SP-B protein was associated with reduced lung compliance. These findings demonstrate the critical role of SP-B in perinatal adaptation to air breathing.

Original languageEnglish
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume273
Issue number4 17-4
Publication statusPublished - 1997
Externally publishedYes

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Surface-Active Agents
Lung
Lung Compliance
IgA receptor
Parturition
Phosphatidylcholines
Pulmonary Surfactant-Associated Proteins
Pulmonary Surfactant-Associated Protein D
Pulmonary Surfactant-Associated Protein A
Fluorocarbons
Respiratory Insufficiency
Phospholipids

Keywords

  • Lung compliance
  • Lung function
  • Perfluorocarbon
  • Respiratory distress syndrome
  • Surfactant protein

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Tokieda, K., Whitsett, J. A., Clark, J. C., Weaver, T. E., Ikeda, K., McConnell, K. B., ... Iwamoto, H. S. (1997). Pulmonary dysfunction in neonatal SP-B-deficient mice. American Journal of Physiology - Lung Cellular and Molecular Physiology, 273(4 17-4).

Pulmonary dysfunction in neonatal SP-B-deficient mice. / Tokieda, Keisuke; Whitsett, Jeffrey A.; Clark, Jean C.; Weaver, Timothy E.; Ikeda, Kazushige; McConnell, Keith B.; Jobe, Alan H.; Ikegami, Machiko; Iwamoto, Harriet S.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 273, No. 4 17-4, 1997.

Research output: Contribution to journalArticle

Tokieda, K, Whitsett, JA, Clark, JC, Weaver, TE, Ikeda, K, McConnell, KB, Jobe, AH, Ikegami, M & Iwamoto, HS 1997, 'Pulmonary dysfunction in neonatal SP-B-deficient mice', American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 273, no. 4 17-4.
Tokieda K, Whitsett JA, Clark JC, Weaver TE, Ikeda K, McConnell KB et al. Pulmonary dysfunction in neonatal SP-B-deficient mice. American Journal of Physiology - Lung Cellular and Molecular Physiology. 1997;273(4 17-4).
Tokieda, Keisuke ; Whitsett, Jeffrey A. ; Clark, Jean C. ; Weaver, Timothy E. ; Ikeda, Kazushige ; McConnell, Keith B. ; Jobe, Alan H. ; Ikegami, Machiko ; Iwamoto, Harriet S. / Pulmonary dysfunction in neonatal SP-B-deficient mice. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 1997 ; Vol. 273, No. 4 17-4.
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